FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC136CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC136CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815722
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGAGTACGATATATACTGAAACGAAAACGTTCTAATCTCTTGTTCC201772.473514260985973No Hit
CGTATGAGTACGATATATACTGATTCGAACTTTGAATTGTATGGGGACGG35540.43568764848808783No Hit
CGTATGAGTACGATATATACTGAAAAACCACCTTTCTTATTCTATTGCCT31490.3860383807228443No Hit
CGTATGAGTACGATATATACTGATCAGCAAAAGCAGGTCTCCCCATGCCT23240.2849009834232741No Hit
CGTATGAGTACGATATATACTGATTCAAAATGGAAGATTTTGTACGACAA22380.2743581759471977No Hit
CGTATGAGTACGATATATACTGTGGGGAGGGGCCAGGAGGCTGAGGGAGT15880.19467416595359693No Hit
CGTATGAGTACGATATATACTGGGGGACCTCCATTCACTACAACACAGAA13920.17064637217091116No Hit
CGTATGAGTACGATATATACTGAGAAGAGGTCAGATTGTAGAGATACTAT13790.16905269197103914No Hit
CGTATGAGTACGATATATACTGCCTAGCTTGCTTCCAGCATAGTTTGATT13650.1673364209865616No Hit
CGTATGAGTACGATATATACTGAAGACCCAGATGAAGGCACAGCTGGAGT12650.15507734252600763No Hit
CGTATGAGTACGATATATACTGAAGAAATCAGGAGCATATGGCATATATA12470.15287070840310793No Hit
CGTATGAGTACGATATATACTGTCTGGCTGTCAGTAAGTATGCTAGAGTC11230.137669451112021No Hit
CGTATGAGTACGATATATACTGTGTGGGGAGGGGCCAGGAGGCTGAGGGA11020.1350950446353047No Hit
CGTATGAGTACGATATATACTGAAAAGCATGTGGCGATAGTCCTAGCTAC10790.13227545658937725No Hit
CGTATGAGTACGATATATACTGAGGCACCTGGGCCTCCTGCATTTGCTTG10440.12798477912818337No Hit
CGTATGAGTACGATATATACTGCGGCAACAACAAGAGGATCACTTGAATC9890.1212422859748787No Hit
CGTATGAGTACGATATATACTGGAGGGGAAAAAGAGCTCTTTTTCCCATC8940.10959616143735244No Hit
CGTATGAGTACGATATATACTGAGAACAGAATGGTTCTGGCCAGCACTAC8740.10714434574524165No Hit
CGTATGAGTACGATATATACTGGGACCCCCAAGATCAAAGGTCCCAGGTT8400.10297625906865329No Hit
CGTATGAGTACGATATATACTGGCTTGCCAGTATCTCTGAGGAACATAGA8240.10101480651496465No Hit
CGTATGAGTACGATATATACTGGGGGGAACAACTAGGTCAGAAGTTTGAA8170.10015667102272587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA409.094947E-1295.000019
TAGTACG201.7435252E-595.000016
AGTACGA792950.094.946087
GAGTACG794700.094.934256
GTACGAT792050.094.922048
CGTATGA803450.094.905411
ATGAGTA795200.094.8745654
TACGATA792500.094.790229
TGAGTAC794700.094.742985
GTATGAG805800.094.628632
TATGAGT796600.094.588563
AACGATA1700.092.205889
GTACGAA1050.090.476198
GACGATA6000.090.259
GTACGGT1700.089.411768
GGACGAT6100.087.9918068
GAGGACG6050.087.933896
AGTACGG1750.086.857147
AAGTACG800.083.1256
GTTCGAT800.083.1258
Produced by FastQC (version 0.11.5)