FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC134CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC134CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14505
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTATATAGTCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAAC1491.027231988969321No Hit
CGTCGTATATAGTCTGTAGTCGCTCACCGGGCACGGTGAGCGTGAACACA1250.8617718028266115No Hit
CGTCGTATATAGTCTGTAGTCGGGGGTCACAGCCCCCATCCTGTTGTATA1020.703205791106515No Hit
CGTCGTATATAGTCTGTAGTCGGCAATCTGTTCACAGGTTGCACATATAA810.5584281282316442No Hit
CGTCGTATATAGTCTGTAGTCGGTGGTCACAGCCCCCATCCTGTTGTATA660.4550155118924508No Hit
CGTCGTATATAGTCTCTAGTCCCCAAGGCCATTAGAGGCCAATACAGTGG570.3929679420889349No Hit
CGTCGTATATAGTCTGTAGTCGTTGTAGTTGAATACTTGTGAGGGGTAAT450.3102378490175801No Hit
CGTCGTATATAGTCTGTAGTCGGCAACCTGTTCACAGGTTGCACATATAA450.3102378490175801No Hit
CGTCGTATATAGTCTGTACGCGTGATCAGTAGAAACAAGGTCGTTTTTAA310.21371940710099968No Hit
CGTCGTATATAGTCTGTAGTCGAGTCCCATACAACTGGCAAGTGCACCAG310.21371940710099968No Hit
CGTCGTATATAGTCTGTAGTCGGGGGACTCGAACTGTGTTATCATTCCAT310.21371940710099968No Hit
CGTCGTATATAGTCTGTAGTCGGGGGGCACAACCCCCATCCTGTTGTATA310.21371940710099968No Hit
CGTCGTATATAGTCTGTAGTCGAGTCCCGTTTTCGTTTCATTACCAACAC300.2068252326783868No Hit
CGTCGTATATAGTCTGTAGTCGAACCTCTCAAATGAAGATACTGAACTCA280.19303688383316098No Hit
CGTCGTATATAGTCTGTAGTCGCTCATACAGAAGACCATAGCCTCTACCA250.1723543605653223No Hit
CGTCGTATATAGTCTGTAGTCGCCAAAAGGAATGGAACCTTCTTCCACTC250.1723543605653223No Hit
CGTCGTATATAGTCTGTAGTCGGCCTTTGTGGCCATTTCTTCGCCGGTGA240.1654601861427094No Hit
CGTCGTATATAGTCTGTAGTCGAAAGCTTAAGCTTTCGATAAAGTTTGAC240.1654601861427094No Hit
CGTCGTATATAGTCTGTAGTCGAAACAAGGTCGTTTTTAAATTATTCGAA240.1654601861427094No Hit
CGTCGTATATAGTCTGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTA220.15167183729748362No Hit
CGTCGTATATAGTCTGTTGTCTTTTGTGTATATAGCCCATCCACTGATAG220.15167183729748362No Hit
CGTCGTATATAGTCTGTAGTCGCAAAGCAAGAGAGACAGCCCCATCCCTC210.14477766287487073No Hit
CGTCGTATATAGTCTGTACGCGTGATCAGTAGAAACAAGGCCGTTTTTAA200.13788348845225784No Hit
CGTCGTATATAGTCTGTAGTCGAAATTAATTGATGGCCATCCGAATTCTT190.13098931402964495No Hit
CGTCGTATATAGTCTGTAGTCGCGGCCAGAACCAATCCATTAATAAGACA190.13098931402964495No Hit
CGTCGTATATAGTCTGTACGCGTGATCAGTAGAAACAAGGCAGTTTTTTA180.12409513960703206No Hit
CGTCGTATATAGTCTGTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTA180.12409513960703206No Hit
CGTCGTATATAGTCTGTAGTCGTGTTGTATTTCCGTTATCCTTTTATCAG170.11720096518441916No Hit
CGTCGTATATAGTCTGTAGTCGCGTGATCAGTAGAAACAAGGTCGTTTTT170.11720096518441916No Hit
CGTCGTATATAGTCTGTAGTCGTTTTTAAATTATTCGAAATTAATTGATG170.11720096518441916No Hit
CGTCGTATATAGTCTGTAGTCGAAAGGGGGAAAAGCTAATATGCTAGAGT160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGCACAGCGACTTGAAGATGTATTTGCTGG160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGAAACGTACGTTTCGACCTCGGTTAGAAG160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGCGGTGCTCCAGTTTCGGTATTTTTTGTC160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGAAGAAAGTTGCTCCAGATTGTCCCTAAG150.1034126163391934No Hit
CGTCGTATATAGTCTGTAGTCGGGGTGACATTGAGAGTTAGTAGCTCCTA150.1034126163391934No Hit
CGTCGTATATAGTCTGTAGTCGGGGGCAAGAATGGAATGATAACACAGTT150.1034126163391934No Hit
CGTCGTATATAGTCTGTAGTCGGATGTCAATCCGACATTACTTTTCTTAA150.1034126163391934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG201.6861553E-595.000016
TCGGATA201.6861553E-595.000013
CGGATAT201.6861553E-595.000014
CGTCGGA201.6861553E-595.000011
GTCGGAT201.6861553E-595.000012
TCGCATA156.4857816E-495.03
CGCATAT156.4857816E-495.04
ATATAGT14000.094.6607067
CGTATAT13750.094.654554
ATAGTCT14000.094.321429
CGTCGTA13950.094.3191
GTCGTAT13850.094.314072
TCGTATA13800.094.31163
TATAGTC14100.093.9893658
TATATAG13850.093.9711156
GTATATA13900.093.6330955
GGATATA255.101612E-576.000015
CGTCGCA200.002032892571.251
GCATATA200.002032892571.255
TGTTGGT403.983587E-947.50000466-67
Produced by FastQC (version 0.11.5)