FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC117CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC117CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23750
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTATCACTACTAGTACTCTCGGGGGACTCGAACTGTGTTATCATTCCA2791.174736842105263No Hit
CGCTATCACTACTAGTACTCTCCGTCCCCATGCAACTGATTCGAACTTTG2671.1242105263157895No Hit
CGCTATCACTACTAGTACTCTCCAGCAACAACATTAGTGTTGTTAATATT1820.7663157894736842No Hit
CGCTATCACTACTAGTCCTCTCCAGCAACAACATTAGTGTTGTTAATATT1460.6147368421052631No Hit
CGCTATCACTACTAGTACTCTCAGACGGCCAAATTCCTCATTTTTGCCGT1270.5347368421052632No Hit
CGCTATCACTACTAGTACTTGCCACTGGTCCATTTCTGTTCCACCATGTC1250.5263157894736842No Hit
CGCTATCACTACTAGTACTCTCAAACCCCTTTCTAAGTCTGTTACTTTTG1240.5221052631578947No Hit
CGCTATCACTACTAGTACTCTCATGCCCAGCCACTTTTTGCTTGGGCATG1040.4378947368421053No Hit
CGCTATCACTACTAGTACTCTCCGGCAACAACATTAGTGTTGTTAATATT920.3873684210526316No Hit
CGCTATCACTACTAGTACTCTTGAACTGAGAAGCAGATACTGGGCCATAA780.32842105263157895No Hit
CGCTATCACTACTAGTACTCTCTTGTATATGGGTCCTCCAGTTTTCTTAG770.32421052631578945No Hit
CGCTATCACTACTAGTACTCTCCAGCGACAACATTAGTGTTGTTAATATT670.28210526315789475No Hit
CGCTATCACTACTAGTACTCTCCGTTTGCTGCAATCCAGTGACTGTTGAT660.27789473684210525No Hit
CGCTATCACTACTAGTACTCTCAATCCAAACTGGAAGTCAAAACAACACT640.2694736842105263No Hit
CGCTATCACTACTAGTACTCTCCTGCAACAACATTAGTGTTGTTAATATT640.2694736842105263No Hit
CGCTATCACTACTAGTACTCTCCCGCAACAACACTAATGTTGTTGCTGGA620.26105263157894737No Hit
CGCTATCACTACTAGTACTCTCATGCCCAAGCAAAAAGTGGCTGGCCCTC600.25263157894736843No Hit
CGCTATCACTACTAGTACTCTCCAAACACCATTTTCATACAAATATGATA590.24842105263157893No Hit
CGCTATCACTACTAGTACTCTCAACCCAGAAGCAAGGTCTTATACAGTCC520.21894736842105264No Hit
CGCTATCACTACTAGTACTCTCCTGCAACAACACTAATGTTGTTGCTGGA480.20210526315789473No Hit
CGCTATCACTACTAGTACTCTCGATATTCTTCCCTCATAGACTCTGGTAC450.1894736842105263No Hit
CGCTATCACTACTAGTACTCTCCGGCAACAACATTAGTGTTGTTAATCAT440.18526315789473682No Hit
CGCTATCACTACTAGTACTCTCCATCGACAGTCACTGGATACATCTGCAG390.16421052631578947No Hit
CGCTATCACTACTAGTACTCTCGGGGGCAGAATGGAATGATAACACAGTT370.15578947368421053No Hit
CGCTATCACTACTAGTACTCTCCAAAGGAGATGTGTTTGTCATTTAATAG340.14315789473684212No Hit
CGCTATCACTACTAGTACTCTCAAAAGGGGAAGGTTACTAAATCAATAGA340.14315789473684212No Hit
CGCTATCACTACTAGTACTCTCAGGCACATGGTAACAACAACCATTCTGT330.13894736842105262No Hit
CGCTATCACTACTAGTACTCTCATGCCCACAAAGAGGGCCAGCCACTTTT320.13473684210526315No Hit
CGCTATCACTACTAGTACTCTCTGTCCATATTATTTGGATCCCCATTCCC320.13473684210526315No Hit
CGCTATCACTACTAGTACTCTCAATCCCAACTGGAAGTCAAAACAACACT320.13473684210526315No Hit
CGCTATCACTACTAGTACTCTCTTTGCCTAAAATGAGGAATCCCCTTAGA310.13052631578947368No Hit
CGCTATCACTACTAGTACTCTCAGTCCAAACTGGAAGTCAAAACAACACT310.13052631578947368No Hit
CGCTATCACTACTAGTACTCTCTTGTTCCACTTCAAATAATAGCTGTAAT300.12631578947368421No Hit
CGCTATCACTACTAGTACTCTCTTAAGCTTTCGATAAAGTTTGACTGCTC300.12631578947368421No Hit
CGCTATCACTACTAGTACTCTCATGCCCAAACAAAGAGGGCCAGCCACTT300.12631578947368421No Hit
CGCTATCACTACTAGTACTCTCCAGCCACAACATTAGTGTTGTTAATATT300.12631578947368421No Hit
CGCTATCACTACTAGTACTCTCAGGCAAATGGTAACAACAACCAATCCAT290.12210526315789474No Hit
CGCTATCACTACTAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAA290.12210526315789474No Hit
CGCTATCACTACTAGTACTCTCAAAACTATTCTCTGTCGTCTTCAATTTA290.12210526315789474No Hit
CGCTATCACTACTAGTACTCTCCTTCCCCTTTTCAAGATCGAAAAGGGGA280.11789473684210526No Hit
CGCTATCACTACTAGTACTCCCAGGACACTGAAATTTCTTTCACCATAAC280.11789473684210526No Hit
CGCTATCACTACTAGTACTCTCAGGCGAATGGTAACAACAACCATTCTGT270.11368421052631579No Hit
CGCTATCACTACTAGTACTCTCATGCCAGTTGTTCCTGTTACCCAGACAC270.11368421052631579No Hit
CGCTATCACTACTAGTACTCTCAAGCCAAACTGGAAGTCAAAACAACACT270.11368421052631579No Hit
CGCTATCACTACTAGTCCTCTCCATACTCTTTCATTGCTTTTTCTGCAAG260.10947368421052632No Hit
CGCTATCACTACTAGTACTCTCATGCGCAAGCAAAAAGAGGGCCAGCCAC260.10947368421052632No Hit
CGCTATCACTACTAGTACTCTCATACAGAAGACCATAGCCTCTACCATAC260.10947368421052632No Hit
CGCTATCACTACTAGTACTCTCACGCGACGCGTGATCAGTAGAAACAAGG250.10526315789473684No Hit
CGCTATCACTACTAGTACTCTCAAATGAAGATACTGAACTCAATTGCTCC250.10526315789473684No Hit
CGCTATCACTACTAGTACTCTCGAAAAGGGAAAGGTTACTAAATCAATAG250.10526315789473684No Hit
CGCTATCACTACTAGTACTCTCAAGCCCCTTTCTAAGTCTGTTACTTTTG240.10105263157894737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTATC201.7087457E-595.000011
CACTATC201.7087457E-595.000011
ACTATCA201.7087457E-595.000012
CGATCAC156.537717E-495.03
CTACTAG23250.094.18289
CTATCAC23050.094.1757053
GCTATCA22800.094.1666642
CGCTATC22650.094.161151
TCACTAC23300.093.980696
ACTACTA23400.093.782058
CACTACT23300.093.7768257
TATCACT23300.093.572974
ATCACTA23400.093.376075
ACTTCTA255.16966E-576.08
TCACTTC255.16966E-576.06
CGCGATC200.002049079271.251
CACTTCT301.2752741E-463.3333367
CTTCTAG301.2752741E-463.3333369
CGACAAC205.3358625E-447.50000426-27
CCTACGC205.3358625E-447.50000422-23
Produced by FastQC (version 0.11.5)