FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC106CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC106CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32469
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTATACGTCACGTGCTGTACCCCGACCAATCAGTTATTGCTACAACA3180.9793957313129447No Hit
CGACTATACGTCACGTGCTGTACGCGTGATCAGTAGAAACAAGGTCGTTT2300.7083679817672242No Hit
CGACTATACGTCACGTGCTGTACCCTGACCAATCAGTTATTGCTACAACA2030.6252117404293326No Hit
CGACTATACGTCACGTGCTGTACCCAGACCAATCAGTTATTGCTACAACA1620.49893744802734913No Hit
CGACTATACGTCACGTGCTGTAGGAGGGGCAAGAGTTCTGCTGAGGACAC970.298746496658351No Hit
CGACTATACGTCACGTGCTGTACGCGTGATCAGTAGAAACAAGGCCGTTT940.2895069142874742No Hit
CGACTATACGTCACGTGCTGTAACGAAAACGTTCTAATCTCTTGTTCCAC920.2833471927068897No Hit
TGACTATACGTCACGTGCTGTACCCCGACCAATCAGTTATTGCTACAACA710.2186701161107518No Hit
CGACTATACGTCACGTGCTGTACTGACAGCAATCTACTCCCCTGGAATTC700.21559025532045953No Hit
CGACTATACGTCACGTGCTGTAATCCCCTTTTCTGTCTTGATAAGTCTGG580.17863192583695217No Hit
CGACTATACGTCACGTGCTGTAGAAACAAGGTCGTTTTTAAATTATTCGA550.16939234346607532No Hit
CGACTATACGTCACGTGCTGTAGAAAGGGGAAAAAGCTAATGTGCTAATT490.15091317872432164No Hit
CGACTATACGTCACGTGCTGTAAAGGGGAAAAAGCTAATGTGCTAATTGG480.14783331793402937No Hit
CGACTATACGTCACGTGCTGTAAGACCCTTTTCTATACTGACACCTCCTG460.14167359635344484No Hit
CGACTATACGTCACGTGCTGTACCCCCATTCGTTTCTGATAGGCCTGTAA440.13551387477286025No Hit
TGACTATACGTCACGTGCTGTACCCTGACCAATCAGTTATTGCTACAACA440.13551387477286025No Hit
CGACTATACGTCACGTGCTGTACTCCCCTGGAATTCAAGCAAAACTGTAA430.132434013982568No Hit
CGACTATACGTCACGTGCTGTACCAACACTACATCCCCTTGCCCAATTAG380.11703471003110659No Hit
CGACTATACGTCACGTGCTGTAGAGGGGGCAAACGGAGTAAAAGGGTTTT370.11395484924081431No Hit
CGACTATACGTCACGTGCTGTATCAGTAGAAACAAGGTCGTTTTTAAATT370.11395484924081431No Hit
CGACTATACGTCACGTGCTGTAAGGGGAAAAAGCTAATGTGCTAATTGGG370.11395484924081431No Hit
CGACTATACGTCACGTGCTGTAGGAAGGGGAAAAAGCTAATGTGCTAATT370.11395484924081431No Hit
CGACTATACGTCACGTGCTGTATTGGGCAAGGGGATGTAGTGTTGGTAAT360.11087498845052204No Hit
CGACTATACGTCACGTGCTGTAGGGGAGCAAACGGAGTAAAAGGGTTTTC340.10471526686993747No Hit
CGACTATACGTCACGTGCTGTAAAAGGGGAAAAAGCTAATGTGCTAATTG330.1016354060796452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATG201.7183018E-595.000019
CTACACG201.7183018E-595.000014
ACACGTC201.7183018E-595.000016
ACGCCAC201.7183018E-595.000018
CACGTCA201.7183018E-595.000017
TACACGT201.7183018E-595.000015
TTATACG409.094947E-1295.000014
CCTCACG201.7183018E-595.000019
ACCTCAC201.7183018E-595.000018
GATTATA409.094947E-1295.000012
AGTCACG201.7183018E-595.000019
CGACTAC201.7183018E-595.000011
GACTACA201.7183018E-595.000012
CGATTAT409.094947E-1295.000011
CTTTACG156.559617E-495.04
CGTTACG156.559617E-495.09
TTCGTCA156.559617E-495.07
ACGTCAT156.559617E-495.08
ACGTTAC156.559617E-495.08
CATCACG550.095.09
Produced by FastQC (version 0.11.5)