FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC099CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC099CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112217
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT37603.3506509708867642No Hit
CGTACGTCTACTACTCTACTGTATGAGCTACTTGGGAAGAATTTTTCAAA16851.5015550228574992No Hit
CGTACGTCTACTACTCTCCTGTCTTCCTGATGTGTATTTCTTGATTATGG12601.1228245274780113No Hit
CGTACGTCTACTACTCTACTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC10840.9659855458620352No Hit
CGTACGTCTACTACTCTACTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG8900.793106213853516No Hit
CGTACGTCTACTACTCTACTGTCTTCCCGATGTGTATTTCTTGATTATGG7950.7084488090039833No Hit
CGTACGTCTACTACTCTACTGTAGACCACATGGCCATAATCAAGAAATAC7560.6736947164868068No Hit
CGTACGTCTACTACTCTACTGTAGGCCACATGGCCATAATCAAGAAATAC7370.6567632355169003No Hit
CGTACGTCTACTACTCTACTGTCTTCCTGATGTGTATTTCTTGATTATGG6960.6202268818449967No Hit
CGTACGTCTACTACTCTACTGTCTCCTCTGACTAAGGGCATTTTGGACAA4410.39298858461730396No Hit
CGTACGTCTACTACTCTACTGTCAGAAACGAATGGGGGTGCAGATGCAAC4030.35912562267749093No Hit
CGTACGTCTACTACTCTACTGTCGCCTCTGACTAAGGGCATTTTGGACAA3950.3519965780585829No Hit
CGTACGTCTACTACTCTACTGTAATTGGGTATTTCATTGCCATCATCCAT3220.2869440459110474No Hit
CGTACGTCTACTACTCTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAC2910.2593189980127788No Hit
CGTACGTCTACTACTCTACTGTCACCCCTGACTAAGGGCATTTTGGACAA2660.23704073357869127No Hit
CGTACGTCTACTACTCTACTGTCGGCAACCTGTGAACAGATTGCCGACTC2620.2334762112692373No Hit
CGTACGTCTACTACTCTACTGTCATCTCTGACTAAGGGCATTTTGGACAA2520.22456490549560226No Hit
CGTACGTCTACTACTCTACTGTCCTCGCTCACTGGGCACGGTGAGCGTGA2510.22367377491823875No Hit
CGTACGTCTACTACTCTGCTGTTCCTCTCGATATTCTTCCCTCATAGACT2440.21743586087669425No Hit
CGTACGTCTACTACTCTACTGTTTCGGTATTTTTTGTCCATCTTCCTCTT2380.2120890774125133No Hit
CGTACGTCTACTACTCTACTATTTGCTTTCCAACACAAGTAGCTGTTTCG2310.20585116337096873No Hit
CGTACGTCTACTACTCTTCTGTATGAGCTACTTGGGAAGAATTTTTCAAA2290.20406890221624174No Hit
CGTACGTCTACTACTCTACTGTGAGGGCGAAACGTACGTTCTCTCTATCA2170.19337533528787973No Hit
CGTACGTCTACTACTCTACTGTAATGCTTGCATAAATGTTATTTGCTCAA2090.18624629066897172No Hit
CGTACGTCTACTACTCTACTGTAGCCCACATGGCCATAATCAAGAAATAC2060.18357289893688122No Hit
CGTACGTCTACTACTCTACTGTTGATGGAGCAAACGGAGTAAAAGGGTTT1840.1639680262348842No Hit
CGTACGTCTACTACTCTCCTATTGTGACTGGGTGTACGTTCTGGAAAGGA1760.15683898161597618No Hit
CGTACGTCTACTACTCTACTGTCACCACTGACTAAGGGCATTTTGGACAA1750.15594785103861267No Hit
CGTACGTCTACTACTCTACTGTCACCGCTGACTAAGGGCATTTTGGACAA1710.1523833287291587No Hit
CGTACGTCTACTACTCTACTGTGATGGGTTTTTCTCCTGTCTTCCTGATG1660.14792767584234118No Hit
CGTACGTCTACTACTCTACTGTCACCCTGACTAAGGGCATTTTGGACAAA1650.14703654526497767No Hit
CGTACGTCTACTACTCTACTGTTCCACTTCAAATAATAGCTGTAATGCTT1640.14614541468761416No Hit
CGTACGTCTACTACTCTACTGTGCAGATGCAACGATTCAAGTGATCCTCT1630.14525428411025068No Hit
CGTACGTCTACTACTCTACTGTAGCCACATGGCCATAATCAAGAAATACA1620.14436315353288717No Hit
CGTACGTCTACTACTCTACTGTACGCGTGATCAGTAGAAACAAGGTACTT1610.1434720229555237No Hit
CGTACGTCTACTACTCTACTGTTGCGGTGCTCCAGTTTCGGTATTTTTTG1610.1434720229555237No Hit
CGTACGTCTACTACTCTACTGTAACCACATGGCCATAATCAAGAAATACA1600.14258089237816018No Hit
CGTACGTCTACTACTCTACTGTACGCGTGATCAGTAGAAACAAGGTCGTT1580.14079863122343317No Hit
CGTACGTCTACTACTCTACTGTTGTTGCCGCAAGTATAATTGGGATTGTG1560.13901637006870615No Hit
CGTACGTCTACTACTCTACTGTAAGAAACGAATGGGGGTGCAGATGCAAC1530.13634297833661566No Hit
CGTACGTCTACTACTCTACTGTCACCTCTGACAAAGCGTCTACGCTGCAG1520.13545184775925215No Hit
CGTACGTCTACTACTCTACTGTTGCCGCAAGTATAATTGGGATTGTGCAC1440.12832280314034414No Hit
CGTACGTCTACTACTCTACTGTCAGGAACGAATGGGGGTGCAGATGCAAC1420.12654054198561715No Hit
CGTACGTCTACTACTCTACTGTATGCTAGAGTCCCGTTTTCGTTTCATTA1400.12475828083089015No Hit
CGTACGTCTACTACTCTACTGTGAAAGGGGAAAAAGCTAATGTGCTAATT1360.12119375852143616No Hit
CGTACGTCTACTACTCTACTGTATGAACGGGCCATCGGTCATTATGGTGA1340.11941149736670914No Hit
CGTACGTCTACTACTCTACTATTATTGATTCGCCTTGCTCATTGATGAAA1340.11941149736670914No Hit
CGTACGTCTACTACTCTACTGTCCCGTTTTCGTTTCATTACCAACACTAC1330.11852036678934563No Hit
CGTACGTCTACTACTCTACTGTCAGCTCTGACTAAGGGCATTTTGGACAA1330.11852036678934563No Hit
CGTACGTCTACTACTCTACTGTGCGGGCAATGAGTCCCAATGGCTCTCAT1330.11852036678934563No Hit
CGTACGTCTACTACTCTACTGTGGGGGGCTATATGAAGCAATTGAGGAGT1330.11852036678934563No Hit
CGTACGTCTACTACTCTACTGTACGCGTGATCAGCAAAAGCAGGTCAATT1320.11762923621198214No Hit
CGTACGTCTACTACTCCACTGCAGATGTATCCTATTTGATAATCCAAGTT1320.11762923621198214No Hit
CGTACGTCTACTACTCTACTGTCAACCCAGAAGCAAGGTCTTATACAGTC1320.11762923621198214No Hit
CGTACGTCTACTACTCTACTATCAGAAACGAATGGGGGTGCAGATGCAAC1310.11673810563461864No Hit
CGTACGTCTACTACTCTACTGTCGGAAACGAATGGGGGTGCAGATGCAAC1300.11584697505725515No Hit
CGTACGTCTACTACTCTACTGTGGGGCCGCAAGTATAATTGGGATTGTGC1300.11584697505725515No Hit
CGTACGTCTACTACTCTACTGTCTGAAAAATGATCTCCTTGAAAATTTAC1260.11228245274780113No Hit
CGTACGTCTACTACTCTACTGTAGCCACATGGCCATCATCCATTTCATCC1220.10871793043834713No Hit
CGTACGTCTACTACTCTTCTGACCTAGTTGTTCCCGCCATTTTCCGTTTC1200.10693566928362012No Hit
CGTACGTCTACTACTCTACTGTCGCTGTCTGGCTGTCAGTAAGTATGCTA1180.10515340812889314No Hit
CGTACGTCTACTACTCTACTGTCGGCCAGACAGCGACCAAAAGAATTCGG1180.10515340812889314No Hit
CGTACGTCTACTACTCTACTGTCAGCGAGACAGCGACCAAATTAATTGAT1130.10069775524207564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCT156.602236E-495.03
GCACGTC254.5933484E-795.02
GTAGGTC301.2218152E-895.02
TCCTACT156.602236E-495.09
GGACGTC156.602236E-495.02
CATACGT450.095.01
CGTACAT450.095.01
ACGTCGA156.602236E-495.04
CGGCTAC254.5933484E-795.05
CGTCGAC156.602236E-495.05
ACATCTA450.095.04
CTCCTAC156.602236E-495.08
ATACGTC450.095.02
CACGTCT254.5933484E-795.03
CGACTAC156.602236E-495.08
AGTACGT156.602236E-495.01
TACGTCG156.602236E-495.03
AAGTCTA301.2218152E-895.04
AGGTCTA301.2218152E-895.04
TAATACT201.7369492E-594.999999
Produced by FastQC (version 0.11.5)