FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC082CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC082CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78365
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGATCGTCTCATACGTAGCCCCCCAAGATCAAAGGTCCCAGGTTCCA10451.3335034773176802No Hit
CGTAGATCGTCTCATACGTAGCCCCCGAAGATCAAAGGTCCCAGGTTCCA7420.9468512728896828No Hit
CGTAGATCGTCTCATACGTAGCAGGCACTGATTCAAAATGGAAGATTTTG5320.6788744975435462No Hit
CGTAGATCGTCTCATACGTAGCCGCCCAAGATCAAAGGTCCCAGGTTCCA5200.6635615389523384No Hit
CGTAGATCGTCTCATACGTAGCCCACCAAGATCAAAGGTCCCAGGTTCCA5110.6520768200089326No Hit
CGTAGATCGTCTCATACGTAGCCCCCTAAGATCAAAGGTCCCAGGTTCCA4990.6367638614177248No Hit
CGTAGATCGTCTCATACGTAGCCCCACAAGATCAAAGGTCCCAGGTTCCA4790.6112422637657118No Hit
CGTAGATCGTCTCATACGTAGCCCCCAAGATCAAAGGTCCCAGGTTCCAG4650.5933771454093026No Hit
CGTAGATCGTCTCATACGTAGCCTCCCAAGATCAAAGGTCCCAGGTTCCA4480.5716837874050915No Hit
CGTAGATCGTCTCATACGTAGCCACCCAAGATCAAAGGTCCCAGGTTCCA4140.5282970713966695No Hit
CGTAGATCGTCTCATACGTAGCCCTCCAAGATCAAAGGTCCCAGGTTCCA4080.5206405921010656No Hit
CGTAGATCGTCTCATACGTAGCACCCCAAGATCAAAGGTCCCAGGTTCCA3460.4415236393798252No Hit
CGTAGATCGTCTCATACGTAGCCCGCCAAGATCAAAGGTCCCAGGTTCCA3300.42110636125821477No Hit
CGTAGATCGTCTCATACGTAGCAGAAAAAGCAATGAAAGAGTATGGAGAG3040.3879282843105979No Hit
CGTAGATCGTCTCATACGTAGCCTAAAATGAGGAATCCCCTTAGAACCGC2980.3802718050149939No Hit
CGTAGATCGTCTCATACGTAGCAGGACACTGTTGGCTAAGTCAGTATTCA2840.36240668665858483No Hit
CGTAGATCGTCTCATACGTAGCACGCGTGATCAGTAGAAACAAGGTCGTT2810.3585784470107829No Hit
CGTAGATCGTCTCATACGTAGCAAGGCCGTTTTTAAATTATTCGAAATTA2570.32795252982836726No Hit
CGTAGATCGTCTCATACGTAGCAAAAGCAGGTACTGATTCAAAATGGAAG2150.2743571747591399No Hit
CGTAGATCGTCTCATACGTAGCGAAGGGGGAAAAAGCTAATGTGCTAATT2110.2692528552287373No Hit
CGTAGATCGTCTCATACGTAGCAGGCCAATTATATTCAATATGGAAAGAA1950.24883557710712692No Hit
CGTAGATCGTCTCATACGTAGCGAAAGGGGAAAAAGCTAATGTGCTAATT1890.241179097811523No Hit
CGTAGATCGTCTCATACGTAGCAAGGTCGTTTTTAAATTATTCGAAATTA1830.2335226185159191No Hit
CGTAGATCGTCTCATACGTAGCGGAAGGGGAAAAAGCTAATGTGCTAATT1820.23224653863331843No Hit
CGTAGATCGTCTCATACGTAGCCCCCCCAGATCAAAGGTCCCAGGTTCCA1770.22586613922031518No Hit
CGTAGATCGTCTCATACGTAGCGGAGGGGGAAAAAGCTAATGTGCTAATT1720.21948573980731192No Hit
CGTAGATCGTCTCATACGTAGCAAAGGGGAAAAAGCTAATGTGCTAATTG1720.21948573980731192No Hit
CGTAGATCGTCTCATACGTAGCGAGGGGGGAAAAAGCTAATGTGCTAATT1720.21948573980731192No Hit
CGTAGATCGTCTCATACGTAGCCGTCCAGATTCGAAATCAATCCGTGCAT1640.20927710074650674No Hit
CGTAGATCGTCTCATACGTAGCGGGAGGGGAAAAAGCTAATGTGCTAATT1630.20800102086390607No Hit
CGTAGATCGTCTCATACGTAGCAAAAGGGGAAAAAGCTAATGTGCTAATT1580.20162062145090281No Hit
CGTAGATCGTCTCATACGTAGCAGGCCCTCTTTTTAAACCGTGTTTAAAG1500.19141198239009763No Hit
CGTAGATCGTCTCATACGTAGCGAGAGGGGAAAAAGCTAATGTGCTAATT1460.186307662859695No Hit
CGTAGATCGTCTCATACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCG1330.16971862438588656No Hit
CGTAGATCGTCTCATACGTAGCCGTCCCTCATAATCATTGAGCTTAAGCT1310.16716646462068527No Hit
CGTAGATCGTCTCATACGTAGCAGGTACTGATTCAAAATGGAAGATTTTG1300.1658903847380846No Hit
CGTAGATCGTCTCATACGTAGCCACGCGTGATCAGTAGAAACAAGGTCGT1300.1658903847380846No Hit
CGTAGATCGTCTCATACGTAGCCCCCAAAGATCAAAGGTCCCAGGTTCCA1270.16206214509028266No Hit
CGTAGATCGTCTCATACGTAGCCAAAGGGGAAAAAGCTAATGTGCTAATT1270.16206214509028266No Hit
CGTAGATCGTCTCATACGTAGCGGAGGGGAAAAAGCTAATGTGCTAATTG1190.15185350602947745No Hit
CGTAGATCGTCTCATACGTAGCGTGATCAGTAGAAACAAGGTCGTTTTTA1190.15185350602947745No Hit
CGTAGATCGTCTCATACGTAGCCACAAAAAGAAATGCTGCTCCCACTAGT1140.1454731066164742No Hit
CGTAGATCGTCTCATACGTAGCAGAAGGGGAAAAAGCTAATGTGCTAATT1130.14419702673387355No Hit
CGTAGATCGTCTCATACGTAGCAGCAAAAGCAGGTACTGATTCAAAATGG1130.14419702673387355No Hit
CGTAGATCGTCTCATACGTAGCGAGGGGGAAAAAGCTAATGTGCTAATTG1120.14292094685127288No Hit
CGTAGATCGTCTCATACGTAGCTTGCAGAAAAAGCAATGAAAGAGTATGG1120.14292094685127288No Hit
CGTAGATCGTCTCATACGTAGCTCCAGTTTCGGTATTTTTTGTCCATCTT1100.1403687870860716No Hit
CGTAGATCGTCTCATACGTAGCCAGCACAAAATGCTATAAGCACAACTTT1080.1378166273208703No Hit
CGTAGATCGTCTCATACGTAGCGGGCAGAAAAAGCAATGAAAGAGTATGG1070.13654054743826965No Hit
CGTAGATCGTCTCATACGTAGCGCAAGGGGAAAAAGCTAATGTGCTAATT1060.13526446755566898No Hit
CGTAGATCGTCTCATACGTAGCCATCCGAATTCTTTTGGTCGCTGTCTGG1060.13526446755566898No Hit
CGTAGATCGTCTCATACGTAGCGAAGGGGAAAAAGCTAATGTGCTAATTG1020.1301601480252664No Hit
CGTAGATCGTCTCATACGTAGCGGCAGAAAAAGCAATGAAAGAGTATGGA1020.1301601480252664No Hit
CGTAGATCGTCTCATACGTAGCAATTGGGTTAAGTTGCGGTGCTCCAGTT1000.12760798826006509No Hit
CGTAGATCGTCTCATACGTAGCACGCGTGATCAGTAGAAACAAGGCCGTT990.12633190837746444No Hit
CGTAGATCGTCTCATACGTAGCAAAGGGGGAAAAGCTAATGTGCTAATTG980.12505582849486377No Hit
CGTAGATCGTCTCATACGTAGCCGGCCCTCTTTTTAAACCGTGTTTAAAG970.12377974861226312No Hit
CGTAGATCGTCTCATACGTAGCCCCAGCTATCTCAATGCATGTGTTAGGA960.1225036687296625No Hit
CGTAGATCGTCTCATACGTAGCCACTCCATGAGAGCCTCAAGATCGGTAT960.1225036687296625No Hit
TGTAGATCGTCTCATACGTAGCCCCCCAAGATCAAAGGTCCCAGGTTCCA960.1225036687296625No Hit
CGTAGATCGTCTCATACGTAGCGGGGGGAAAAAGCTAATGTGCTAATTGG950.12122758884706183No Hit
CGTAGATCGTCTCATACGTAGCAAGGGGAAAAAGCTAATGTGCTAATTGG940.11995150896446118No Hit
CGTAGATCGTCTCATACATAGCCCCCCAAGATCAAAGGTCCCAGGTTCCA910.11612326931665921No Hit
CGTAGATCGTCTCATACGTAGCGGGGGGGGAAAAAGCTAATGTGCTAATT900.11484718943405856No Hit
CGTAGATCGTCTCATACGTAGCAGGCGCTGATTCAAAATGGAAGATTTTG890.11357110955145792No Hit
CGTAGATCGTCTCATACGTAGCGGGGGGGAAAAAGCTAATGTGCTAATTG870.11101894978625662No Hit
CGTAGATCGTCTCATACGTAGCAGGAAAGCAATGAAAGAGTATGGAGAGG860.10974286990365596No Hit
CGTAGATCGTCTCATACGTAGCAACAGCGTCATACTCCATATTTTTGGCT830.10591463025585403No Hit
CGTAGATCGTCTCATACGTAGCGGAAAAGCAATGAAAGAGTATGGAGAGG820.10463855037325337No Hit
CGTAGATCGTCTCATACGTAGCGAAAAAGCAATGAAAGAGTATGGAGAGG790.10081031072545141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC450.095.000012
AGCTCGT201.7336606E-595.000014
CGGAGAT201.7336606E-595.000011
TGATCGT450.095.000014
GCTCGTC201.7336606E-595.000015
TCGTCTA450.095.000017
CTCGTCT201.7336606E-595.000016
TAGAACG301.2169039E-895.03
CGTCACA156.5947324E-495.08
AACGTCT301.2169039E-895.06
GTCACAT156.5947324E-495.09
TCGATCG156.5947324E-495.03
AAATCGT500.095.04
ACGTCTC301.2169039E-895.07
TTCGTCT500.095.06
GTCGCAT156.5947324E-495.09
GAACGTC301.2169039E-895.05
GGATCGT254.5803245E-795.04
GTACATC156.5947324E-495.02
ACCGTCT156.5947324E-495.06
Produced by FastQC (version 0.11.5)