FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC070CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC070CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGAGCTACGTCGTGCACGTATTTGGGCATTAATTTCTTTACTTGTTGT150.7598784194528876No Hit
CGTGAGCTACGTCGTGCACGTAATTGGGTATCTCATTGCCATCATCCACT50.25329280648429586No Hit
CGTGAGCTACGTCGTGCACGTACACGTGCACACACACTCATATTCACACA40.2026342451874367No Hit
CGTGAGCTACGTCTTGCACATATTACTGCCCAAGCATAGTATCTACACTC40.2026342451874367No Hit
CGTGAGCTACGTCGTGCACGTAATGCCCAAATAGCACTGGCTTCACTAGT40.2026342451874367No Hit
CGTGAGCTACGTCGTGCATGTGTGTGAATGTGTATGCCTGTGTGAATGTA30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGGTGCGGTGTGCAAGTGTGAGTGAATGT30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGCCAAGGAGGTGTCACTAAGCTA30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGGGGCGAGAAGGGGATTGAACTCAGCAA30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTACACTCATATTCACACACACCCATTCACA30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGCTCGCGAGATACTCACTAAGACCACTG30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGGGCAAAACCGCTAATAATCTTGC20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTAGGGGGCTGCGCGCGGCGTAGAAAACTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGCGGGTGTGTGAGAGTGTGTGTGTATGT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACAAGAAGGAATTTGATGTGACCTGCTTC20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTAGGATGGGCAAAACCGCTAATAATCTTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAACCGCTAATAATCTTGCCGCCAACTAC20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTAGGGGAGGCAAGAGAAGAACCCCGCACTC20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTGTGCAAAGGGACCGCCACTTTGTCTGGAG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGGGGGGCAAGTGTGAGTGAATGTGTGT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGAAAGAGAAAACCTGAAGACACCCACA20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTAGGGGCGGCAAGATTATTAGCGGTTTTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACTAAAAATCAAATAATAGTTGTAAAGTG20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTAGGGGGGCCAAGGAGGTGTCACTAAGCTA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGGAAGGGAAGGAAAGATAATGTTTTAC20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTACTTAAAATCAAATAATAGTTGTAAAGTG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACATGAGGGATAATATCTTATAGTCTGTT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCTCTTACTGGTTTAATATCATGTGCATCCTCTGA20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTAGGAGGGGCAAAACCGCTAATAATCTTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGGAGGGGAACAGTGACCACAGAAGCTG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGAGGAACACGTACAATGCTTGTTT20.10131712259371835No Hit
CGTGAGCTACGTCCTGCACATATACCCCTGACTGAACTTAAAATTTAAAA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTGTGCAAAGGGACCGCCACTTTGTCTGGAG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGAGGCAAGAGAAGAACCCCGCACTC20.10131712259371835No Hit
CATGAGCTACGTCGTGCACGTAATGTCCCTTCCACCACGACTGCTTAGAA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAAGTGCTTGAGCAATGTAGTAAGCTTCT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGCGGGGAAATGAAGCAGGGGGCTGCG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGAGGCAAGAAGGGGATTGAACTCAGCAA20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTATTTGGGCATTAATTTCTTTACTTGTTGT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTCAGTGGTCCAAGCCATTGGTACATCAAAT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGAGGTGATACCATCACTCGGTCTGATCC20.10131712259371835No Hit
CGTAAGCTACGTCGTGCACGTAGGGGGGAGAAGGGGATTGAACTCAGCAA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGTGTGTGAGAGTGTGTGTGTATGTG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGTATGCTAGAGTCCCGTTTTCGTTTCA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACTCAGAATCAAATAATAGTTGTAAAGTG20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTAGACGGGGAAATGAAGCAGGGGGCTGCGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACACTCTTATTCACACACACCCATTCACA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACGCGTGATCAGTAGAAACAAGGTCGTTT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAACACTCATATTCACACACACCCATTCAC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACTCAAAATCAAATAATAGTTGTAAAGTG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTATTGGTGATATAACCATAGTCCTTGCCAT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACACAAATTGGTTTGGCAGATTTTAACAG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGTGTGTGAGAGTGTGTGTGTATGTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGGAAGGCAAGAGAAGAACCCCGCACTC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTATGAGGGATAATATCTTATAGTCTGTTCG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGTGTGAGAGTGTGTGTGTATGTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACAACAAGTAAAGAAATTAATGCCCAAAT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGCGGACAAAATGTTGCCATGGCAATTT20.10131712259371835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAGC351.546141E-1095.01
ACGTCGT1850.095.09
GAGCTAC1750.095.04
GCTACGT1900.095.06
GTGAGCT1650.095.02
TACGTCG1850.095.08
CTACGTC1900.095.07
CGTGAGC1300.095.01
TGAGCTA1750.095.03
AGCTACG1950.094.999995
CACGTAG505.456968E-1247.50000416-17
TGCACGT1850.047.514-15
GTGCACG1800.047.514-15
GTCGTGC1850.047.510-11
CACGTAT301.1433749E-647.516-17
CACGTAA600.047.516-17
ACGTACC150.00957628147.518-19
ACGTAAT150.00957628147.518-19
TCGTGCA1800.047.512-13
GCACGTA1850.047.516-17
Produced by FastQC (version 0.11.5)