FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC060CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC060CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAGCGAGACAGTCGTGTCGGGCAAGAGAAGAACCCCGCACTCAGAAT11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGAGAGGGGGAAGGGGCCCCTCCCACGTGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGTTGGGCAAAGGTGGATTTGGAGATAATG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCAGCTCGCGAGATACTCACTAAGACCCC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCTCGCGGGATACTCACTAAGACCCCCGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGAAAGGTAACGCGAAGTAGGTACAGATG11.9607843137254901No Hit
TGCGAGCGAGACAGTCGTGTCGAGAGAGGGAATGTAAACCAAAAGTATGT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGGGGAAGGGAACACTTATGCTGAT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAGACGGGGACGCGGGGGGGACAGGCCCG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAGGGGAGGAAAGTGACACAGCGAGAGCA11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGCTAAGCTATTCAACTGGTGCACTTGCCA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCGAAACAGAATGGTGCTGCCTAGTCTGA11.9607843137254901No Hit
AGCGAGCGAGACAGTCGTGTCGAGGGAGCGAAACAAACGAATCTGGATCT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAGCGGGGCAATTAACCGAAAGCCACACA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGGTTTGTCCTTGTTCATTCCTTTCTGGA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGAGGGGCGAAGAAACTCCACCACATTTC11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGGCGCGGGCAACATAAAAAAAAAGAAAGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGTTCTAGGAATTTGTCCATTTCATCTAAG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGCCACGCAAAGAGGGCCTATTATCTTTT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGCACGGGGATTTCTCGAATAGATTGCAC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGTTTGCTTTGCTCCAGAGGGTTTGTCCTT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAACAGACAAATGGCTACTACCACCAATC11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGTGAGGAAGGAGTTGAACCAAGATGCATT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGACCAAAACAATTCGACACTAATTGATGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGTCCCCACAACTTGAGGGGTTTTCGGGCG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGGACAGGGCCTGATTCGGGCTCTTCCTT11.9607843137254901No Hit
CGCGAGCGAGACAGGCGTCTCGCGAGCGAGGCGCGGGGTCAGTAGAAAAA11.9607843137254901No Hit
AGCGAGCGAGACAGTCGTGTCGACGGAGAGAATAAAAGAACTGTAAGTCG11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGCAGTCCCGCACTCGCGAGATACTCACTA11.9607843137254901No Hit
CGCGAGCGAGACAGTCATGTAGAACACAGGAAGGCAAGAGAAGAACCCCG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCCACGCACATCGATCGAGGTAGCGCCCC11.9607843137254901No Hit
CCCGAGCGAGACAGTCGTGTCGAGCCGAGATCAGACCATTGCACTCCAGC11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGAGGAAGGCAAGATTGCTCAGTTCATTGA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGGGAAAATCGAAACTAATAAGTTT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCGCGCGCGAGACACGCGTGATCAGTAGA11.9607843137254901No Hit
CCCGAGCGAGACAGTCGTGTCGGCAGGGAAAAGAAAATAGTACTGAAAGC11.9607843137254901No Hit
CGCTAGCGAGACAGTCGTGTCGAGGCCGTTTTTAAACAATTCGACACTAA11.9607843137254901No Hit
CGCGAGCGAGACAGTCATGTCAGAAAGGGAACGCGAAGTAGGTACAGATG11.9607843137254901No Hit
AGCGAGCGAGACAGTCGTGTCGAAGGACAGAAATTTGAGGAAACAAGGGT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCGAGCGGGAAGAATAAGTCCTTCATTAC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAAAGAGAGAAAGCTAATGTGCTAATTGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGATGTACAGTCTCAATTTTGTATTTAATG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGATCAGCAAAAGCAGGCTTGCCCTGGGCC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGTATGGGCTTGCAGTAGCAAGTGGGCATG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGACCAGGGCAATCAGCTTCTGAAATCCTA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGACGAAAGCTCTCTCTGCAATGTTTTTGT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTGGTTGATGATAGAACTGCAACAATGATGAG11.9607843137254901No Hit
AGCGAGCGAGACAGTCGTGTCGGCAAGGCAAAAAAAAAACAAAAAATGGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTGAGAAGGAGAAACAAGGTCGTTTTTAAACA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCAGGGAGCAACCTTCATAATCGTCCCCA11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGCGGGGGGGAATCAGTTTTTGGATGTGCT11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAG50.094.999994
CGAGCGA50.094.999993
CGAGACA50.094.999997
AGCGAGA50.094.999995
AGACAGT50.094.999999
GCGAGCG50.094.999992
GCGAGAC50.094.999996
GAGACAG50.094.999998
CGCGAGC50.094.999991
Produced by FastQC (version 0.11.5)