FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC050CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC050CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20685
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGTACACACTACGTAGCAAGGCGATCTTCCAGGAGCTAATGGTAGT880.4254290548706793No Hit
CGAGAGTACACACTACGTAGCACCGCCCATAGTTGACCTGCACAGTATGA580.28039642252840224No Hit
CGAGAGTACACACTACGTAGCAAGAGAGGAAATGTAAACCAAAAGTATGT470.22721779066956732No Hit
CGAGAGTACACACTACGTAGCACGGATATAAAGAATCAGGCCAAACCTAA440.21271452743533964No Hit
CGAGAGTACACACTACGTAGCAATTCGACACTAATTGATGGCCATCCGAA390.1885424220449601No Hit
CGAGAGTACACACTACGTAGCACGTATATAAAGAATCAGGCCAAACCTAA360.1740391588107324No Hit
CGAGAGTACACACTACGTAGCACTGCCCAATTAGCACATTAGCTTTCTCT360.1740391588107324No Hit
CGAGAGTACACACTACGTAGCACACATATAAACGTATTCTTATATGTTCA340.1643703166545806No Hit
CGAGAGTACACACTACGTAGCAGGTGAAAGATGTGAGATAAAAACAATTA340.1643703166545806No Hit
CGAGAGTACACACTACGTAGCAACCTCCATGGCCTCTGCTGCCTGTTCAC330.15953589557650472No Hit
CGAGAGTACACACTACGTAGCAAGCCCATACACACAAGCAGGCAGGCAGG310.14986705342035292No Hit
CGAGAGTACACACTACGTAGCACGCGTGATCAGTAGAAACAAGGTCGTTT300.145032632342277No Hit
CGAGAGTACACACTACGTAGCAAAGGGGAGAAGTTTTATGTTCATTCCTT290.14019821126420112No Hit
CGAGAGTACACACTACGTAGCAGAAACGAGAAAGCTCTTATCTCTTGTTC290.14019821126420112No Hit
CGAGAGTACACACTACGTAGCACATCCAGAAACTGATTGCCCCCAGGGAG290.14019821126420112No Hit
CGAGAGTACACACTACGTAGCAGTGTTCTCTCTTTTATTCCTGATTTTAG280.1353637901861252No Hit
CGAGAGTACACACTACGTAGCACGCATATAAAGAATCAGGCCAAACCTAA280.1353637901861252No Hit
CGAGAGTACACACTACGTAGCACTCCCATTCGCTTCTGGTAGGCCTGCAA260.1256949480299734No Hit
CGAGAGTACACACTACGTAGCAATACACACATATAAACGTATTCTTATAT260.1256949480299734No Hit
CGAGAGTACACACTACGTAGCAGGAGAGGAAATGTAAACCAAAAGTATGT250.1208605269518975No Hit
CGAGAGTACACACTACGTAGCACGTCTATAAAGAATCAGGCCAAACCTAA240.1160261058738216No Hit
CGAGAGTACACACTACGTAGCACCCCAAGATCGAAGGTTCCAGGTTCCAG240.1160261058738216No Hit
CGAGAGTACACACTACGTAGCAAAACGAGAAAGCTCTTATCTCTTGTTCT230.11119168479574572No Hit
CGAGAGTACACACTACGTAGCAGAGAGAGGAAATGTAAACCAAAAGTATG220.10635726371766982No Hit
CGAGAGTACACACTACGTAGCAATGCCCAATTAGCACATTAGCTTTCTCT220.10635726371766982No Hit
CGAGAGTACACACTACGTAGCACGTACATAAAGAATCAGGCCAAACCTAA220.10635726371766982No Hit
CGAGAGTACACACTACGTAGCAAGGAGCTTGCTTCTGTAGTGCTGTGTTG220.10635726371766982No Hit
CGAGAGTACACACTACGTAGCATGGGCATTCTTGCTAAACATTTATCCAC220.10635726371766982No Hit
CGAGAGTACACACTACGTAGCACGAATATAAAGAATCAGGCCAAACCTAA210.10152284263959391No Hit
CGAGAGTACACACTACGTAGCAAAACCCAGGGATCATTAATCAGGCACTC210.10152284263959391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC201.703411E-595.000013
CGAGAAT201.703411E-595.000011
GCGAGTA201.703411E-595.000012
CGCGAGT201.703411E-595.000011
CAAGAGT550.095.01
TAAACAC156.5254717E-495.07
GGGTACA156.5254717E-495.04
AAGAGTA550.095.02
AGAGGAC700.095.03
AGGGTAC156.5254717E-495.03
GGACACA700.095.06
CGAGGGT156.5254717E-495.01
GACACAC700.095.07
GAGTAAA156.5254717E-495.04
AACACTA156.5254717E-495.09
GGAGTAC156.5254717E-495.03
GTAAACA156.5254717E-495.06
AGAGTAC17650.094.730883
CGAGAGT16850.094.71811
CACACTA19450.094.5115669
Produced by FastQC (version 0.11.5)