FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC047CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC047CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCATAGTGTACGTCGACTCAGGCCCTCCTTCCGTAGAAGGCCCTCTT60.36607687614399026No Hit
CGCTCATAGTGTACGTCGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTT50.3050640634533252No Hit
CGCTCATAGTGTACGTCGACTCAGGCGCTCCTTCCGTAGAAGGCCCTCTT40.24405125076266015No Hit
CGCTCATAGTGTACGTCGACTCTCTGGTTGTTAGTCCATTGTAATACTTT40.24405125076266015No Hit
CGCTCATAGTGTACGTCGACTCCCGCTATATCCTGACCACTCATTTATTC40.24405125076266015No Hit
CGCTCATAGTGTACGTCGACTCAGTCCAGTTCATAGTAAGGGACAAAGCT30.18303843807199513No Hit
TGCTCATAGTGTACGTCGACTCTCTGGTTGTTAGTCCATTGTAATACTTT30.18303843807199513No Hit
CGCTCATAGTGTACGTCCACTTCTTCCCCGGTCAGTAATATTGCCAAGTG30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCTGGTTGTTAGTCCATTGTAATACTTTGC30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCCAGCTCTGACATCTATCTTAAGCGCAAG30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCAGTAGCAGTGTTCTCTCTTTTATTCCTG30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCAGTAGAAACAAGGTAGTTTTTTACTCTA30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCCACCCCAGATGTGCCTTCATCTGGATCT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAAAGCCAAAATAACCAGGAAAAAAATC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAGGCTCTCCTTCCGTAGAAGGCCCTCTT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCTGCATTGTCTCCGAAGAAATAAGACCCT20.12202562538133008No Hit
TGCTCATAGTGTACGTCGACTCAGAAGCGAATGGGAGTGCAGATGCAGCG20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAGAGCAGAAATGAAAAGTGGCGAGAGC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCACAAAGCCAAAATAACCAGGAAAAAAAT20.12202562538133008No Hit
TGCTCATAGTGTACGTCGACTCGAGAAGCGAATGGGAGTGCAGATGCAGC20.12202562538133008No Hit
CGCTCATAGTGTACGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGA20.12202562538133008No Hit
TGCTCATAGTGTACGTCGACTCCAGAAGCAAATGGGAGTGCAGATGCAGC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCTGCCCCATTACCTTATGAAAGCACAGC20.12202562538133008No Hit
TGCTCATAGTGTACGTCGACTCAGGCCCTCCTTCCGTAGAAGGCCCTCTT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCCAGGCCAAACCTAACAAGGCTAGTCTT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAGTAAACTCGTCTCTTGAATCTTTATTA20.12202562538133008No Hit
TGCTCATAGTGTACGTCGACTCATCCAAGCTCCAGTGCTGGTCTGAAAGA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCATTAAATAAGCTGAAACGAGAAAGCTCT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAGAAAAATTATTTAGTATATATACTTAT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCTATTGGGAAAGTGTGCAGGGGCAAAATC20.12202562538133008No Hit
CGCTCATAGTGTACGTCTTCTCTCTTTTTTTCATGGGCATTCTTGCTAAA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCATTACCAACACTACGTCCCCTTGCCCAA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAGGCAAATTATTTAGTATATATACTTAT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCACACCATGTCAAGCTTTCAGGTAGACT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCTGGCTTTGATACCAGAGTAATTCTGGTT20.12202562538133008No Hit
TGCTCATAGTGTACGTCGACTCAGGCCAAACCTAACAAGGCTAGTCTTAC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAGCCCTTTACTTCGATTTTTGTCCATT20.12202562538133008No Hit
CGTTCATAGTGTACGTCGACTCATCACAGTTTCTCCAAGCGAATCTCTGT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCGAAGGCAAAAAGGTCAATAATATAATCA20.12202562538133008No Hit
TGCTCATAGTGTACGTCGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTT20.12202562538133008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA1450.095.07
GTGTACG1450.095.09
CGCTCAT850.095.01
ACTCATA155.4655084E-495.02
ATAGTGT1450.095.06
AGTGTAC1450.095.08
TGCTCAT252.8333125E-795.01
CACTCAT155.4655084E-495.01
CATAGTG1350.095.05
GCTCATA1150.095.02
TCATAGT1300.095.04
CTCATAG1350.091.4814763
TCGACTC1500.047.516-17
ACGTCGA1550.047.512-13
ACTCTTT150.00941632647.518-19
GTCGACT1500.047.514-15
TACGTCG1550.047.512-13
CTCAGCA150.00941632647.520-21
CGTCGAC1500.047.514-15
TGTACGT1500.047.510-11
Produced by FastQC (version 0.11.5)