FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC046CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC046CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9545
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACTACGACTGCCCATACACACAAGCAGGCAGGCAGGATTTATGTGC380.39811419591409114No Hit
CGAGACTACGACTGCACGTACAATGAACTGGCGACAGTTGAATAGATCGC280.2933473022524882No Hit
CGAGACTACGACTGCACGTACACGAAGAAATAAGACCCTTCATTACTCAT280.2933473022524882No Hit
CGAGACTACGACTGCACGTACAGAAACGAGAAAGCTCTTATCTCTTGTTC200.20953378732320588No Hit
CGAGACTACGACTGCACGTACACCACAAAAGGATATGCTGCTCCCGCTAG190.19905709795704557No Hit
CGAGACTACGACTGCACGTACAAGCCCATACACACAAGCAGGCAGGCAGG190.19905709795704557No Hit
CGAGACTACGACTGCACGTACAATAAGCTGAAACGAGAAAGCTCTTATCT180.1885804085908853No Hit
CGAGACTACGACTGCACGTACAATTAGCACATTAGCTTTCTCTCCTTTTG180.1885804085908853No Hit
CGAGACTACGACTGCACGTACAAAACGAGAAAGCTCTTATCTCTTGTTCT180.1885804085908853No Hit
CGAGACTACGACTGCACGTACACATCCAGAAACTGATTGCCCCCAGGGAG170.17810371922472498No Hit
CGAGACTACGACTGCACGTACAGAAAGCACCATCCTCTCTATTGTTATGC160.1676270298585647No Hit
CGAGACTACGACTGCACGTACAAAAGCAGAAAGCACCATCCTCTCTATTG160.1676270298585647No Hit
CGAGACTACGACTGCACGTACAATTCGACACTAATTGATGGCCATCCGAA160.1676270298585647No Hit
CGAGACTACGACTGCACGTACACAGAGCAGAAATGAAAAGTGGCGAGAGC140.1466736511262441No Hit
CGAGACTACGACTGCACGTACAAGAAGCTCAAAAGAGAAATAACGTTCCA140.1466736511262441No Hit
CGAGACTACGACTGCACGTACAATGAGCTGGCGACAGTTGAATAGATCGC130.1361969617600838No Hit
CGAGACTACGACTGCACGTACACGCCCCTGGAAGGACAAATCTTCTGGCT130.1361969617600838No Hit
CGAGACTACGACTGCACGTACAATGCCAAGGAAATTGGAAACGGCTGCTT120.12572027239392353No Hit
CGAGACTACGACTGCACGTACACACAGCAGGCAGGCAGGATTTATGTGCA120.12572027239392353No Hit
CGAGACTACGACTGCACGTACACAAATCCTAAAATTCCCTTAGTCAGAGG120.12572027239392353No Hit
CGAGACTACGACTGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACC110.11524358302776323No Hit
CGAGACTACGACTGCACGTACACCATCCTCTCTATTGTTATGCTATTCTG110.11524358302776323No Hit
CGAGACTACGACTGCACGTACAAGGCACCAAACGATCATATGAACAAATG110.11524358302776323No Hit
CGAGACTACGACTGCACGTACAATAACTTCTCAATAAAGCAAGATATCGT110.11524358302776323No Hit
CGAGACTACGACTGCACGTACAAGGCCCTCTTTTCAAACCGTATTTAAAG110.11524358302776323No Hit
CGAGACTACGACTGCACGTACAAGAAACTGATTGCCCCCAGGGAGACTAC110.11524358302776323No Hit
CGAGACTACGACTGCACGTACAAAAACGAGAAAGCTCTTATCTCTTGTTC100.10476689366160294No Hit
CGAGACTACGACTGCACGTACAAGGGGGTTTTTTATCATTAAATAAGCTG100.10476689366160294No Hit
CGAGACTACGACTGCACGTACAACCCACAGTGTCACTGTTTACACCACAA100.10476689366160294No Hit
CGAGACTACGACTGCACGTACAATCCGACTCTACTTTTCCTAAGCACCAC100.10476689366160294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACT1050.095.01
CAAGACT550.095.01
CGATACT156.4159936E-495.01
GATACTA156.4159936E-495.02
ATTACGA156.4159936E-495.05
AAGACTA550.095.02
TACGACT9050.095.07
TACGACC156.4159936E-495.07
AGAGACT156.4159936E-495.01
CTACGAC9000.095.06
ACGACTG9000.095.08
ACGACCG156.4159936E-495.08
GATTACG156.4159936E-495.04
ATACTAC156.4159936E-495.03
CGACCGC156.4159936E-495.09
CGACTGC9000.095.09
TTACGAC156.4159936E-495.06
TACTACG156.4159936E-495.04
ACTACGA9000.095.05
AAACTAC201.6559565E-594.999993
Produced by FastQC (version 0.11.5)