FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC045CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC045CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCGTCTCGACGTGTCTGTTGAATTGTTCGCATGATAACCTATACAT170.4688361831218974No Hit
CGTGTCGTCTCGACGTGTGTGTTTTTTATCATTAAATAAGCTGAAACGAG130.35852178709321564No Hit
CGTGTCGTCTCGACGTGTGTGTAGTAGCCATTTGTCTGTGAGACCGATGC130.35852178709321564No Hit
CGTGTCGTCTCGACGTGTGTGTTACTGTTACATTCTTTTCTAGTACTGTG130.35852178709321564No Hit
CGTGTCGTCTCGACGTGTGTGTCCCAGTCCATCCGTTCGGATCCCAAATC100.27578599007170435No Hit
CGTGTCGTCTCGACGTGTGTGTTGTTTTCATAAGTAATGACGCTTTGATT100.27578599007170435No Hit
CGTGTCGTCTCGACGTGTGTGTTGCTGATGTTAACATATGTCTGATTTAC90.2482073910645339No Hit
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGAGTTTAAAATGAATCCAAA70.19305019305019305No Hit
CGTGTCGTCTCGACGTGTGTGTTCGGATCCCAAATCATCTCAAAACCGTT60.1654715940430226No Hit
CGTGTCGTCTCGACGTGTGTGTTTTCATAAGTAATGACGCTTTGATTGCA60.1654715940430226No Hit
CGTGTCGTCTCGACGTGTGTGTTTGGTAGGGAGAACTAATGCTTTTAGTT60.1654715940430226No Hit
CGTGTCGTCTCGACGTGTGTGTAAGACCTGCTGTTGCATCTTCGCCATTG60.1654715940430226No Hit
CGTGTCGTCTCGACGTGTGTGTTTATAGCACCCTTGGGTGTCTGACAAGT60.1654715940430226No Hit
CGTGTCGTCTCGACGTGTGTGTCTTCTAGAAGGTTAACAGAGTGTGTTAC60.1654715940430226No Hit
CGTGTCGTCTCGACGTGTGTGTCTGACAAGTTGTATTGCAATCGTGGACT50.13789299503585217No Hit
CGTGTCGTCTCGACGTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATC50.13789299503585217No Hit
CGTGTCGTCTCGACGTGTGTGTAGAAGCTTTTTGCTCCAGCATGAGGACA50.13789299503585217No Hit
CGTGTCGTCTCGACGTGTGTGTCTGACCAGTTGTATTGCAATCGTGGACT50.13789299503585217No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGAGTTTAAAATGAATC50.13789299503585217No Hit
CGTGTCGTCTCGACGTGTGTGTCGCTGGATTCCTCTTTCAAGATCCATTC50.13789299503585217No Hit
CGTGTCGTCTCGACGTGTGTGTTTGGTTAGGGAGAACTAATGCTTTTAGT50.13789299503585217No Hit
TGTGTCGTCTCGACGTGTGTGTGAGACCGATGCTGTGAATCAGCAATCTG40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTCTGATTTACCCAAGTGTTGTTTTCATAA40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTTTCGATATCGAGGCCAAGGGTGTTGCCT40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTTAGTGTTACATTCTTTTCTAGTACTGTG40.11031439602868175No Hit
TGTGTCGTCTCGACGTGTGTGTAGTAGCCATTTGTCTGTGAGACCGATGC40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTAGCAGGGGAAAACAAAAGCAACAAAAAT40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTGGCTGTTTCGATATCGAGGCCAAGGGTG40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTTTTATCATTAAATAAGCTGAAACGAGAA40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTCTGTTATTATGCCGTTGTACTTTAACACAGCC40.11031439602868175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTC201.5174972E-595.000013
GTATCGT201.5174972E-595.000012
CGTATCG201.5174972E-595.000011
ATCGTCT201.5174972E-595.000014
TGTGTCG201.5174972E-595.000011
CTCGACG3250.095.09
GTGTCGT3000.095.02
GTCGTCT3050.095.04
TCTCGAC3300.095.08
GTCTCGA3300.095.07
TCGTCTC3300.095.05
ATGTCGT156.0904375E-495.02
CGTGTCG2850.095.01
TGTCGTC3150.095.03
CATGTCG156.0904375E-495.01
CGTCTCG3300.093.560616
CAGCATT205.037265E-447.50000478-79
TCGACGG205.037265E-447.50000410-11
GTGTACG205.037265E-447.50000418-19
CATTTCT205.037265E-447.50000482-83
Produced by FastQC (version 0.11.5)