FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC044CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC044CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGACGATCGACGCTTTGATTGCATGTTTCAATCTGATTTTGATTC301.1806375442739079No Hit
CGAGTAGACGATCGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCG130.5116096025186935No Hit
CGAGTAGACGATCGACGCGCTGAAACGAGAAAGCTCTTATCTCTTGTTCT130.5116096025186935No Hit
TGAGTAGACGATCGACGCGCTGAAACGAGAAAGCTCTTATCTCTTGTTCT70.27548209366391185No Hit
CGAGTAGACGATCGACGCGCTGATGAGAGAAGAAATAAATACCTAGAAGA60.23612750885478156No Hit
CGAGTAGACGATCGACGCGCTGTTGCATCTTCGCCATTGTTTGCTTGGCG50.19677292404565133No Hit
CAAGTAGACGATCGACGCGCTGAAACGAGAAAGCTCTTATCTCTTGTTCT50.19677292404565133No Hit
CGAGTAGACGATCGACATGCTGCCGTTACACCTTTGTTCGAGTCATGATT50.19677292404565133No Hit
CGAGTAGACGATCGACGCGCTGAGGCCATGGAGGTTGCTAATCAGACTAG50.19677292404565133No Hit
CGAGTAGACGATCGACGCGCTGGGATGCTCTTCTAGGTATTTATTTCTTC40.15741833923652104No Hit
CGAGTAGACGATCGACGCGCTGCATCTGCACTCCCATTCGCTTCTGGTAG40.15741833923652104No Hit
CGAGTAGACGATCGACGCGCTGAACAGAGGTTATAAGAATGATGGAAAGT40.15741833923652104No Hit
CGAGTAGACGATCGACGCGCTGAAGAGCACACAGAATGCCATTGACAAGA40.15741833923652104No Hit
CGAGTAGACGATCGACGCGCTGGAGGGGGGAAAATGGACGAAGGACAAGG40.15741833923652104No Hit
CGAGTAGACGATCGACGCGCTGGATTCCTCTTTCAAGATCCATTCCACGA40.15741833923652104No Hit
CGAGTAGACGATCGACGCGCTGATTAGCAAAAGCAGGAGTTTAAAATGAA30.11806375442739078No Hit
CGAGTAGACGATCGACGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGT30.11806375442739078No Hit
CAAGTAGACGATCGACGCGCTGAGGCCCATGCAACTGGCAAGTGCACCAG30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGCACAGATTGAAAGCGACAGAGAATAGTT30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGCAGTCCTCGCTCACTGGGCACGGTGAGC30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGTTCTGGATTAGTCGTCCATCATAATCAC30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGCAAATTTTCAAGAAGGTCATCTTTCAGA30.11806375442739078No Hit
TGAGTAGACGATCGACGCGCTGATCCGCAAAAGCAGGAGTTTAAAATGAA30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGAAAAGGGGAAGATAGTCAAATCAGTCGA30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGATTTACCCAAGTGTTGTTTTCATAAGTA30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGTGGGGAGCGCCAGGATGCCACAGAAATC30.11806375442739078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTAG201.42436165E-595.000011
GAGTAGA2100.092.73812
CGAGTAG1900.092.51
GACGATC2450.091.1224447
TAGACGA2450.091.1224445
AGACGAT2400.091.041676
ACGATCG2400.091.041678
CGATCGA2400.091.041679
GTAGACG2250.090.777784
AGTAGAC2200.090.6818163
CGCTGAT204.886964E-447.50000418-19
CGCTGAA252.4409333E-547.50000418-19
GCAAAAG204.886964E-447.50000426-27
GCGCTGA800.047.50000416-17
CGCTGTT204.886964E-447.50000418-19
CGCTGGG356.012306E-847.518-19
CGCTGGC150.00973873647.518-19
ATCCTCT150.00973873647.574-75
CGCTGCA150.00973873647.518-19
CGCTGAG356.012306E-847.518-19
Produced by FastQC (version 0.11.5)