FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC035CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC035CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGGGTTTTT200.5473453749315819No Hit
TGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTT190.5199781061850027No Hit
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTT190.5199781061850027No Hit
CAAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTT100.27367268746579093No Hit
CGAGATGACGAGACGCACGACGCTTAGAATGACAATCGCACGCAAAGAGG80.21893814997263275No Hit
CGAGATGACGAGACGCACGACGATTCGAGCCATGCCAGTTATCCCTGCAC70.19157088122605362No Hit
TGAGATGACGAGACGCACGACGAGCAGAAATGAAAAGTGGCGAGAGCAAT60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGCCAAACTCTCCTTATTTCTTCTTTGTCA60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGCAGAGCAGAAATGAAAAGTGGCGAGAGC60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGAGAAAGCTCTTATCTCTTGTTCTACTTC60.16420361247947454No Hit
TGAGATGACGAGACGCACGACGAAGAAATAAGACCCTTCATTACTCATGT60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGAATCCAGAAAGCACCATCCTCTCTATTG60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGCGAAGTAGGTACAGATGCAATTGTCATT60.16420361247947454No Hit
TGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGGGTTTTT60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGCTTAGAATGACAATTGCATCTGTACCTA60.16420361247947454No Hit
TGAGATGACGAGACGCACGACGCAAAGAGGGCCTATTATCTTTTGCCTAG50.13683634373289547No Hit
TGAGATGACGAGACGCACGACGCTTAGAATGACAATTGCATCTGTACCTA50.13683634373289547No Hit
CGAGATGACGAGACGCACGACGCAAAGAGGGCCTATTATCTTTTGCCTAG50.13683634373289547No Hit
CGAGATGACGAGACGCACGACGGCAAAGGCTATGGAACAGATGGCTGGAT50.13683634373289547No Hit
CGAGATGACGAGACGCACGACGATAAGACAGGCAGTTGTGGTCCAGTATC40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAAGCGAATGGGAGTGCAGATGCAGCGAT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGGTGGGGAGCGCCAGGATGCCACAGAAAT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAAAACATAATGGACTCCAACACCATGTC40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAAAGCAGAAAGCACCATCCTCTCTATTG40.10946907498631638No Hit
CGACATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGGGTTTTT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAATAGCATAACAATAGAGAGGATGGGTC40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAAAAGGGAAAGATAGTCAAATCAGTCGA40.10946907498631638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAGA3250.095.000017
GAGATGA2850.095.000012
CAAGATG309.215E-995.01
GATGACG3050.095.04
AAGATGA309.215E-995.02
AGATGAC3050.095.03
TGAGATG750.095.01
ATGACGA3200.094.999995
TGACGAG3200.094.999996
CGAGACG3450.093.6231849
CGAGATG2200.092.840911
ACGAGAC3550.092.323948
TAGAAAC150.00992209347.532-33
ACGCTTA150.00992209347.520-21
CAGTAGA150.00992209347.528-29
ACGCACG3400.047.512-13
GACGGGG509.094947E-1247.518-19
GACGGGA150.00992209347.518-19
CGCTTAG150.00992209347.520-21
GACGGAG150.00992209347.518-19
Produced by FastQC (version 0.11.5)