FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC026CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC026CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13570
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTCTAGATACTCTGTAGAGCCCCGATTGGGCCAAAAGAACCAAAACC1821.3411938098747236No Hit
CGCGTCTAGATACTCTGTAGAGAAAAACCAAAGTTTAATGATATGGGTCC650.47899778924097275No Hit
CGCGTCTAGATACTCTGTAGAGCTTCCCAGCACTGGGATGCTCTTCTAGG570.4200442151805453No Hit
CGCGTCTAGATACTCTGTAGAGCTACCAAATGTTCGCGAATCATTGGGAA420.30950626381724394No Hit
CGCGTCTAGATACTCTGTAGAGGCCAAAAGAACCAAAACCAAAGGCCTTA340.2505526897568165No Hit
CGCGTCTAGATACTCTGTAGAGAAAAGCGAAAGCATAGAAAGTGAGTGTG300.2210759027266028No Hit
CGCGTCTAGATACTCTGTAGAGAAGAGAAAGGCACCACACACTTTTAGAC300.2210759027266028No Hit
CGCGTCTAGATACTCTGTAGAGGGGCCCACCCCTCTCCTCTTTCTCTTCC280.20633750921149593No Hit
CGCGTCTAGATACTCTGTAGAGCCAAAACCAAAGGCCTTAATCCCATTAT250.18422991893883567No Hit
CGCGTCTAGATACTCAGTAGAAACAAGGGTAGAAGAAAAGCGAAAGCATA250.18422991893883567No Hit
CGCGTCTAGATACTCTGTAGAGCATCCCAGCACTGGGATGCTCTTCTAGG240.17686072218128224No Hit
CGCGTCTAGATACTCTGTAGAGACCCATTAGAGCACATCCAGAAACTGAT210.15475313190862197No Hit
CGCGTCTAGATACTCTGTAGAGAAAAGCGAAAGCATAGAAAGTGAGGGTG190.1400147383935151No Hit
CGCGTCTAGATACTCTGTAGAGCCCCGATTGGGCCAAAAAAACCAAAACC190.1400147383935151No Hit
CGCGTCTAGATACTCTGTAGAGAAAAGCGAAAGCATAGAAAGGGAGGGGG190.1400147383935151No Hit
CGCGTCTAGATACTCTGTAGAGGGGCCAGGGGCCCACCCCTCTCCTCTTT180.13264554163596168No Hit
CGCGTCTAGATACTCTGTAGAGAAAAGCGAAAGCATAGAAAGGGAGGGTG170.12527634487840825No Hit
CGCGTCTAGATACTCTGTAGAGAAAAACCAAAGTTCACAGATATCCAGGC170.12527634487840825No Hit
CGCGTCTAGATACTCTGTAGAGAAAACCAAAGTTTAATGATATGGGTCCA160.11790714812085483No Hit
CGCGTCTAGATACTCTGTAGAGGGGGCCAAAAGAACCAAAACCAAAGGCC150.1105379513633014No Hit
CGCGTCTAGATACTCTGTAGAGCTGCCAAATGTTCGCGAATCATTGGGAA150.1105379513633014No Hit
CGCGTCTAGATACTCTGTAGAGATGCAATTGTCATTCTAAGTGTCTCGCT150.1105379513633014No Hit
CGCGTCTAGATACTCTGTAGAGGTGATACCATCACTCGGTCTGATCCAGC150.1105379513633014No Hit
CGCGTCTAGATACTCTGTAGAGACACCCACAACAAGTAAAGAAATTAATG140.10316875460574797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCTA156.476459E-495.02
TCTAGAT11600.095.05
GATACTT156.476459E-495.09
GATACTC12400.095.09
ACGTCTA301.14196155E-895.02
CGCCTAG156.476459E-495.03
TGTCTAG156.476459E-495.03
CACGTCT301.14196155E-895.01
ATCTAGA301.14196155E-895.04
GCGGCTA1000.095.02
CCAGATA156.476459E-495.06
TCCAGAT156.476459E-495.05
GCGCCTA156.476459E-495.02
GTCCAGA156.476459E-495.04
GATTCTC156.476459E-495.09
CAGATAC156.476459E-495.07
CGTGTCT156.476459E-495.01
CGCGGCT1000.095.01
GTCTAGA11250.095.04
GGCTAGA1000.095.04
Produced by FastQC (version 0.11.5)