FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC025CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC025CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52714
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT15993.033349774253519No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT11352.153128201236863No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGGGTTTT9301.7642372045376937No Hit
CGTCTATCATACGACTGTCTACATTCCCATTTAGGGCATTTTGGATAAAG5461.0357779716963236No Hit
CGTCTATCATACGACTGTCTACATGCGAACAGAGGTTATAAGAATGATGG2700.5121978980915886No Hit
CGTCTATCATACGACTGTCTACCCGAGAGCAATTGGGACAGAAATTTGAG2540.48184543005653147No Hit
CGTCTATCATACGACTGTCCAGCAGCAAAGTTGGTGTTGCTGATGTTAAC2430.46097810828242974No Hit
CGTCTATCATACGACTGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGT2230.4230375232386083No Hit
CGTCTATCATACGACTGTCTACCAGAGCAGAAATGAAAAGTGGCGAGAGC2210.41924346473422625No Hit
CGTCTATCATACGACTGTCTACCCGCTAGTCCAGATTGTAATTTCACGGA1970.37371476268164056No Hit
CGTCTATCATACGACTGTCTACCCCTCTTAGTTTGCATAGTTTCCCGTTA1800.34146526539439237No Hit
CGTCTATCATACGACTGTCCATGAGGGAAGAATATCAACAGGAACAGCAG1740.33008308988124596No Hit
CGTCTATCATACGACTGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCT1720.3262890313768638No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGAGTTTAAAATGAATCCAAA1680.31870091436809955No Hit
CGTCTATCATACGACTGTCTACCCGAGGCCAAGGGTGTTGCCTCTTCCTT1640.3111127973593353No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTGACAAA1610.3054217096027621No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGTAGATATTTAAAGATGAGT1590.30162765109837997No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGAGTTTAAA1590.30162765109837997No Hit
CGTCTATCATACGACTGTCTACCCAAAATACTCAGAGGAAGCAAAATTAA1390.26368706605455855No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTATTTT1260.2390256857760747No Hit
CGTCTATCATACGACTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAA1230.23333459801950146No Hit
CGTCTATCATACGACTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT1210.22954053951511932No Hit
CGTCTATCATACGACTGTCTACCTCCAGAGCAGAAATGAAAAGTGGCGAG1180.22384945175854612No Hit
CGTCTATCATACGACTGTCTACCAGAAGCGAATGGGAGTGCAGATGCAGC1150.21815836400197292No Hit
CGTCTATCATACGACTGCCTACCAGAAGCGAATGGGAGTGCAGATGCAGC1090.2067761884888265No Hit
CGTCTATCATACGACTGTCTACGAAACGAGAAAGCTCTTATCTCTTGTTC1060.2010851007322533No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGGTGACAAAAACATAATGGA1050.1991880714800622No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT1040.19729104222787117No Hit
CGTCTATCATACGACTGTCCCCATTCCCATTTAGGGCATTTTGGATAAAG1030.19539401297568007No Hit
CGTCTATCATACGACTGTCTACAAGATCCCAATGATATTTGCTGCAATGA1010.19159995447129793No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTCGTTTT980.18590886671472476No Hit
CGTCTATCATACGACTGTCTACATCCAAATGTGCACTGAACTCAAACTCA930.1764237204537694No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGAGTTTT900.17073263269719618No Hit
CGTCTATCATACGACTGTCTACCTGACCACTCATTTATTCCTACGATATC870.16504154494062298No Hit
CGTCTATCATACGACTGTCTACCATGTTGTTCGGGTCCCCATTCCCATTT870.16504154494062298No Hit
CGTCTATCATACGACTGTCTACACGAGAGCAATTGGGACAGAAATTTGAG870.16504154494062298No Hit
CGTCTATCATACGACTGTCTACCCAAGTGTTGTTTTCATAAGTAATGACG840.15935045718404978No Hit
CGTCTATCATACGACTGTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATA830.1574534279318587No Hit
CGTCTATCATACGACTGTCTACAACACCATGTCAAGCTTTCAGGTAGACT800.1517623401752855No Hit
TGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT790.14986531092309444No Hit
CGTCTATCATACGACTGTCTACCGAAGAAATAAGACCCTTCATTACTCAT760.14417422316652123No Hit
CGTCTATCATACGACTGTCTACCAGAGGCCAAGGGTGTTGCCTCTTCCTT750.14227719391433016No Hit
CGTCTATCATACGACTGTCTACAGGCACTGTAGACACAGTACTAGAAAAG730.13848313540994803No Hit
CGTCTATCATACGACTGTCTACCGGGGAAGAATATCAACAGGAACAGCAG700.13279204765337482No Hit
CGTCTATCATACGACTGTCTACAAGAGAGCAATTGGGACAGAAATTTGAG700.13279204765337482No Hit
CGTCTATCATACGACTGTCTACATGCAATGAGAACTATTGGGACTCATCC700.13279204765337482No Hit
CGTCTATCATACGACTGACTACCCAAAATACTCAGAGGAAGCAAAATTAA700.13279204765337482No Hit
CGTCTATCATACGACTGTCTACCAGGGGCGAATGGGAGTGCAGATGCAGC690.13089501840118373No Hit
CGTCTATCATACGACTGTCTACCCAAATGCAATGGGGCTACCCCTCTTAG670.12710095989680162No Hit
CGTCTATCATACGACTGTCTACAACCGATTAGAGACCTTGATACTACTAA650.12330690139241947No Hit
CGTCTATCATACGACTGTCTACCCCAGATGTGCCTTCCATGTCCATTATT640.12140987214022841No Hit
CGTCTATCATACGACTGTCTACAAGTGGCACACACTAGACCAAAAGCAGC630.11951284288803735No Hit
CGTCTATCATACGACTGTCTACTTTGCCTAAAATGAGAAATCCTCTCAGG630.11951284288803735No Hit
CGTCTATCATACGACTGTCTACCTTCCCAGCACTGGGATGCTCTTCTAGG620.11761581363584625No Hit
CGTCTATCATACGACTGTCTCCCGGCTCTACTAGTGTCCAGTAATAGTTC610.1157187843836552No Hit
CGTCTATCATACGACTGTCTACCAGCACTGGGATGCTCTTCTAGGTATTT610.1157187843836552No Hit
CGTCTATCATACGACTGTCTACCCGACCAGTGAGTACCCTTCCCTTTCAA590.11192472587927306No Hit
CGTCTATCATACGACTGTCTACACCACAAAAGGATATGCTGCTCCCGCTA580.11002769662708199No Hit
CGTCTATCATACGACTGTCTACTAGTGTCCAGTAATAGTTCATTCTCCCT580.11002769662708199No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGGGACAAA570.10813066737489092No Hit
CGTCTATCATACGACTGTCTACATCCGATCACAATTGGAAAATGTCCAAA570.10813066737489092No Hit
CGTCTATCATACGACTGTCTACGGGGGGAAAATGGACGAAGGACAAGGGT570.10813066737489092No Hit
CGTCTATCATACGACTGTCTACCTGCTCATTTTGATGGTGATAACCGTAC570.10813066737489092No Hit
CGTCTATCATACGACTGTCTACGGGGGAAGAATATCAACAGGAACAGCAG560.10623363812269984No Hit
CGTCTATCATACGACTGTCTACCCCATCTATTTCTTCTCTGTTTAATTTT560.10623363812269984No Hit
CGTCTATCATACGACTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAG540.1024395796183177No Hit
CGTCTATCATACGACTGTCTACACGAGGCCAAGGGTGTTGCCTCTTCCTT540.1024395796183177No Hit
CGTCTATCATACGACTGTCTACCGGCTCTACTAGTGTCCAGTAATAGTTC540.1024395796183177No Hit
CGTCTATCATACGACTGTCTACCGGGGGGGAATCAGTTTCTGAAATCCTA530.10054255036612665No Hit
CGTCTATCATACGACTGTCTACCGGGGCAGAAATGAAAAGTGGCGAGAGC530.10054255036612665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAC201.728365E-595.000016
CTATAAT201.728365E-595.000014
GATACGA254.5593515E-795.000018
CGCCTAT254.5593515E-795.000011
ATGATAC254.5593515E-795.000016
TCTATGA254.5593515E-795.000013
ATAATAC201.728365E-595.000016
GCTATCA254.5593515E-795.000013
TCTGTCA254.5593515E-795.000013
ATACGTC201.728365E-595.000019
ATACGAT201.728365E-595.000019
ATATGAC201.728365E-595.000019
GTCTATG254.5593515E-795.000012
GGCTATC254.5593515E-795.000012
TAATACG201.728365E-595.000017
CGGCTAT254.5593515E-795.000011
AATACGA201.728365E-595.000018
CTATGAT254.5593515E-795.000014
CATACGT201.728365E-595.000018
ATCTTAC156.582637E-495.06
Produced by FastQC (version 0.11.5)