FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC019CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC019CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33017
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATACGTACGTCTCAGTCAGGCCAAACCTAACAAGGCTAGTCTTAC970.293788048581034No Hit
CGAGTATACGTACGTCTCAGTCTTTATTTTACTTTGGCAGATTCTGGATT960.2907593058121574No Hit
CGAGTATACGTACGTCTCACTCACCACCAAGGTCATGGTGCTAAGCCATT940.2847018202744041No Hit
CGAGTATACGTACGTCTCAGTCACCACCAAGGTCATGGTGCTAAGCCATT830.25138564981676104No Hit
CGAGTATACGTACGTCTCAGTCCAACAAAAAGCTGGAAGCTGAAAAATTG530.1605233667504619No Hit
CGAGTATACGTACGTCTCAGTCCAGCTATTTATATATTTTCATTGTAATC520.15749462398158526No Hit
CGAGTATACGTACGTCTCAGTCTTGGGCTCCTCGTTGTCTGACAGATCCT440.1332646818305721No Hit
CGAGTATACGTACGTCTCAGTCAGCACCAAGGTCATGGTGCTAAGCCATT400.12114971075506556No Hit
CGAGTATACGTACGTCTCAGTCCCGCACTCGCGAGATACTCACTAAGACC380.11509222521731229No Hit
CGAGTATACGTACGTCTCAGTCTGTGGCCAAAGGCCCGAGAGCCCCTGGC350.10600599691068238No Hit
CGAGTATACGTACGTCTCACTCAGCATCATGACACCAAGACGTTGCAGGA340.10297725414180572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT201.7187373E-595.000019
ATTCGTA201.7187373E-595.000016
TTCGTAC201.7187373E-595.000017
TACGGAC201.7187373E-595.000017
TATTCGT201.7187373E-595.000015
ACGGACG201.7187373E-595.000018
CGTACGG201.7187373E-595.000019
TCGTACG201.7187373E-595.000018
CGTAAGT201.7187373E-595.000019
ACGTATG201.7187373E-595.000018
ACGTAAG201.7187373E-595.000018
ATACGGA201.7187373E-595.000016
ATACCTA201.7187373E-595.000016
TACGTAA201.7187373E-595.000017
AAGTACG301.195076E-895.08
ACTATAC353.1650416E-1095.03
GATACGT301.195076E-895.05
CGAGTTT156.5606146E-495.01
AGTTTAC156.5606146E-495.03
GTACACG156.5606146E-495.04
Produced by FastQC (version 0.11.5)