FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n02_BC009CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n02_BC009CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11113
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCTCTATACGTGTAGTCTCCCAGGGAGACTACCAGTACCAATGAACT1261.1338072527670295No Hit
CGAGCTCTATACGTGTAATCTTCAACCGATTAGAGACCTTGATACTACTA1251.124808782506974No Hit
CGAGCTCTATACGTGTAGTCTCAGTCCCATTTTTACCACAAATGCGATAA980.8818500854854675No Hit
CGAGCTCTATACGTGTAGTCTCCAGCCAGCAATGTTACATTTACCCAAAT490.44092504274273375No Hit
CGAGCTCTATACGTGTAGTCTCCCATTCTCATCACAGTTTCTCCAAGCGA370.33294339962206426No Hit
CGAGCTCTATACGTGTAGTCTCAACCGATTAGAGACCTTGATACTACTAA340.3059479888418969No Hit
CGAGCTCTATACGTGTAGTCTCCAGGGAGACTACCAGTACCAATGAACTG330.2969495185818411No Hit
CGAGCTCTATACGTGTAGTCTCCTAACCGAGGTCGAAACGTACGTTCTTT330.2969495185818411No Hit
CGAGCTCTATACGTGTAGTCTCCGAAGAAATAAGACCCTTCATTACTCAT330.2969495185818411No Hit
CGAGCTCTATACGTGTAGTCTCCGGGGGGGAATCAGTTTCTGAAATCCTA320.2879510483217853No Hit
CGAGCTCTATACGTGTAGTCTCCCACTCTCATCACAGTTTCTCCAAGCGA280.25195716728156214No Hit
CGAGCTCTATACGTGTAGTCTCAGAGGAAGCAAAATTAAACAGAGAAGAA280.25195716728156214No Hit
CGAGCTCTATACGTGTAGTCTCCAGGGGGGAATCAGTTTCTGAAATCCTA280.25195716728156214No Hit
CGAGCTCTATACGTGTAGTCTCCCCAGGGAGACTACCAGTACCAATGAAC270.24295869702150633No Hit
CGAGCTCTATACGTGTAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAA250.22496175650139477No Hit
CGAGCTCTATACGTGTCTTCTCTCTTTTTTTCATGGGCATTCTTGCTAAA250.22496175650139477No Hit
CGAGCTCTATACGTGTAGTCTCAGCCGATTAGAGACCTTGATACTACTAA220.1979663457212274No Hit
CGAGCTCTATACGTGTAGTCTCAGGCCCCCATTCCCATTTAGGGCATTTT200.17996940520111582No Hit
CGAGCTCTATACGTGTAGTCTCGGGAAGCAAAATTAAACAGAGAAGAAAT200.17996940520111582No Hit
CGAGCTCTATACGTGTAGTCTCCCCCCAGGGAGACTACCAGTACCAATGA200.17996940520111582No Hit
CGAGCTCTATACGTGTAGTCTCAGGCCCCCTCAAAGCCGAGATCGCGCAG200.17996940520111582No Hit
CGAGCTCTATACGTGTAGTCTCCCAATCTCATCACAGTTTCTCCAAGCGA200.17996940520111582No Hit
CGAGCTCTATACGTGTAGTCTCTGCGCGATCTCGGCTTTGAGGGGGCCTG190.17097093494106003No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGCTTCTGTGGTCACTGTTCCC190.17097093494106003No Hit
CGAGCTCTATACGTGTAGTCTCCCCCAGGGAGACTACCAGTACCAATGAA190.17097093494106003No Hit
CGAGCTCTATACGTGTAGTCTCATGCCCATTTAGGGCATTTTGGATAAAG190.17097093494106003No Hit
CGAGCTCTATACGTGTAGTCTCCCGCTATATCCTGACCACTCATTTATTC190.17097093494106003No Hit
CGAGCTCTATACGTGTAGTCTCGAGGAAGCAAAATTAAACAGAGAAGAAA180.16197246468100424No Hit
CGAGCTCTATACGTGTAGTCTCCACAGCACGAGGACTTCTTTCCCTTTAT170.15297399442094844No Hit
CGAGCTCTATACGTGTAGTCTCCTGGGTAACACGTTCCATTGTCTGAACT170.15297399442094844No Hit
CGAGCTCTATACGTGTAGTCTCATCAAAAGCAGAAAGCACCATCCTCTCT150.13497705390083686No Hit
CGAGCTCTATACGTGTAGTCTCATCACAGTTTCTCCAAGCGAATCTCTGT150.13497705390083686No Hit
CGAGCTCTATACGTGTAGTCTCAGTCCCAATTTTACCACAAATGCGATAA150.13497705390083686No Hit
CGAGCTCTATACGTGTAGTCTCACAAGTGGCACACACTAGACCAAAAGCA140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCCTGACCACTCATTTATTCCTACGATATC140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCAACCGAGGTCGAAACGTACGTTCTTTCT140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCCATCCCCATTTAGGGCATTTTGGATAAA140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCCATACCCATTTAGGGCATTTTGGATAAA130.11698011338072528No Hit
CGAGCTCTATACGTGTAGAATCTCCCGATTCCACCAATCATTCTTCCGAC130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCCAGCAATGTTACATTTACCCAAATGCAA130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCCCAGGATCCAGCCAGCAATGTTACATTT130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCCTGTTTTCTTAGGGTCCTTCCCAGCACT130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCAGGCACTCCTTCCGTAGAAGGCCCTCTT130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCCCGATTCCACCAATCATTCTTCCGACAG130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCCCGTTATGCTTGTCTTCTAGAAGGTTAA130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCGCCAAGGAAATTGGAAACGGCTGCTTTG120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCAGGCACCATCCTCTCTATTGTTATGCTA120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCCTCATAATTGATGAAATCTCCTGGGTAA120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCCTCTGCATTGTCTCCGAAGAAATAAGAC120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCATTCGCATTTAGGGCATTTTGGATAAAG120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCGGCCAAGGAAATTGGAAACGGCTGCTTT120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCGGGGAAGCAAAATTAAACAGAGAAGAAA120.10798164312066949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC156.4451125E-494.999996
CTATAAG156.4451125E-494.999997
ATAAGTG156.4451125E-494.999999
TATAAGT156.4451125E-494.999998
GAGCCCT201.6685346E-594.999992
TCTATAA156.4451125E-494.999996
ATACGTC201.6685346E-594.999999
CGAGCCC201.6685346E-594.999991
CCCTATA156.4451125E-494.999995
CAAGCTC201.6685346E-594.999991
AGCCCTA201.6685346E-594.999993
ATACGTG10400.092.716359
CGAGCTC10000.092.625011
CTCTATA10700.092.336455
TATACGT10600.092.311328
GAGCTCT10500.092.285722
TCTATAC10550.091.848346
AGCTCTA10750.091.465123
CTATACG10700.091.448597
GCTCTAT11000.090.6818164
Produced by FastQC (version 0.11.5)