FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13250888
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT192410.1452053628405885No Hit
CGACGCGTGTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC152980.11544886652124749TruSeq Adapter, Index 5 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT341000.092.582331
CGTCGTA180750.092.126871
CGAGTAG142400.091.0555951
CGACTAT288400.090.9756851
CGTGTCG423000.090.304891
GCGCACT239650.090.299257
TGTCGCG241850.089.967813
CGCGCAC240450.089.9593056
CGAGATG186750.089.880911
CGCGTCA253300.089.80236
TCGCGCG255250.089.785143
CGCGCGT254700.089.6996464
GCGCGTC255300.089.266245
GTGTCGC244550.089.14932
TCACGTC300900.088.034193
CACGTCG301350.087.840024
CGCTACA84500.087.627951
CGCTATC131450.087.51961
GTCGATA57500.087.1480262
GCGTCAC260950.086.951247
Produced by FastQC (version 0.11.5)