FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC379CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC379CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40649
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT13303.2719132081970033No Hit
CGAGACGATGTGTACGTACTACCCGGGAACAACTAGGTCAGAAGTTTGAA2940.723265024969864No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGGGTTTT2390.5879603434278826No Hit
CGAGACGATGTGTACGTACTACAAGGGCAGAAGTTTGAAGAAGTAAGATG2280.5608994071194864No Hit
CGAGACGATGTGTACGTACTACAAGGGAACAACTAGGTCAGAAGTTTGAA2210.5436788112868706No Hit
CGAGACGATGTGTACGTACTACGGGGGCAGAAGTTTGAAGAAGTAAGATG2120.5215380452163645No Hit
CGAGACGATGTGTACGTACTACCGGGGCAGAAGTTTGAAGAAGTAAGATG2010.49447710890796825No Hit
CGAGACGATGTGTACGTACTACGGGGGAACAACTAGGTCAGAAGTTTGAA2010.49447710890796825No Hit
CGAGACGATGTGTACGTACTACGGGGCGGGAACAACTAGGTCAGAAGTTT1860.4575758321237915No Hit
CGAGACGATGTGTACGTACTACCGGGCGGGAACAACTAGGTCAGAAGTTT1660.4083741297448892No Hit
CGAGACGATGTGTACGTACTACAGGGCGGGAACAACTAGGTCAGAAGTTT1530.3763930231986027No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTATTTT1530.3763930231986027No Hit
CGAGACGATGTGTACGTACTACGAGGGCAGAAGTTTGAAGAAGTAAGATG1390.34195183153337105No Hit
CGAGACGATGTGTACGTACTACCAGGGCAGAAGTTTGAAGAAGTAAGATG1370.33703166129548084No Hit
CGAGACGATGTGTACGTACTACCCGCGCTGGGATGTTCCTCTAGATATTT1280.3148908952249748No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGTAGTTTT1260.30997072498708456No Hit
TGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT1060.26076902260818224No Hit
CGAGACGATGTGTACGTACTACAGGGGCAGAAGTTTGAAGAAGTAAGATG1050.2583089374892371No Hit
CGAGACGATGTGTACGTACTACCAGGCGGGAACAACTAGGTCAGAAGTTT990.24354842677556643No Hit
CGAGACGATGTGTACGTACTACGGGGGGGGAACAACTAGGTCAGAAGTTT980.24108834165662132No Hit
CGAGACGATGTGTACGTACTACCCGGGCAGAAGTTTGAAGAAGTAAGATG970.2386282565376762No Hit
CAAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT910.22386774582400548No Hit
CGAGACGATGTGTACGTACTACGGGGGGAGAATGGAAGAAAAACAAGGAT890.2189475755861153No Hit
CGAGACGATGTGTACGTACTACTAGGTCAGAAGTTTGAAGAAGTAAGATG830.2041870648724446No Hit
CGGGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT820.20172697975349946No Hit
CGAGACGATGTGTACGTACTACAAGGCGGGAACAACTAGGTCAGAAGTTT810.19926689463455435No Hit
CGAGATGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT800.19680680951560925No Hit
CGAGACGATGTGTACGTACTACCAGGGAACAACTAGGTCAGAAGTTTGAA790.19434672439666414No Hit
CGAGACGATGTGTACGTACTACCCGGGGACAACTAGGTCAGAAGTTTGAA790.19434672439666414No Hit
CGAGACGATGTGTACGTACTACGAGGGAACAACTAGGTCAGAAGTTTGAA760.18696646903982878No Hit
CGAGACGATGTGTACGTACTACGGGGGGGGAAGTCTTTGAGCTCTCGGAC750.18450638392088367No Hit
CGAGACGATGTGTACGTACTACGCGGGATCAGTAGAAACAAGGGTGTTTT600.14760510713670694No Hit
CGAGACGATGTGTACGTACTACCCGGCGGGAACAACTAGGTCAGAAGTTT520.12792442618514602No Hit
CGAGACGATGTGTACGTACTACAGGGGAACAACTAGGTCAGAAGTTTGAA490.12054417082831066No Hit
CGAGACGATGTGTACGTACTACAGGGGGAAAATTGATGGAGTGAAATTGG470.11562400059042043No Hit
CGAGACGATGTGTACGTACTACCGGGGAACAACTAGGTCAGAAGTTTGAA470.11562400059042043No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGCGTTTT450.1107038303525302No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGTCGTTTT440.10824374523358507No Hit
CGAGACGATGTGTACGTACTACGGGGGCAGAAATCATAAAGATGATGGAA430.10578366011463998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGTG156.5740064E-494.991396
AGACTAT156.5740064E-494.991393
ACGGTGT156.5740064E-494.991395
TGTGTCC156.5740064E-494.991399
GACTATG156.5740064E-494.991394
GATGTGC201.7243308E-594.991387
GTGACGA201.7243308E-594.991382
CGTGACG201.7243308E-594.991381
TGTGTAA201.7243308E-594.991389
TGATGTG800.094.991386
TGTGTAT500.094.991379
TGTGTAG500.094.991379
TGTGGAC1200.091.033419
ATGTGGA1150.090.861328
GATGTGG1100.090.6735847
ACGATGT38150.090.509485
ATGTGTA38650.090.19888
CGATGTG38700.090.082276
TGTGTAC37900.089.9786459
AGACGAT37750.089.832913
Produced by FastQC (version 0.11.5)