FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC351CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC351CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGCGATACACGTGACGACGCAAAAAGAAATGCTGCTCGCACTAGTCCA13.125No Hit
CGCGCGATACACGTGACGACGCTCAGTCCGAGAGAGGAGAAGAGACAATT13.125No Hit
CGAGCGATACACGTGACGACGCAGGGAACCAATTCATCTCTTTGTCCCAT13.125No Hit
CGTGCGATACACGTGACGACGCTTGGGACATTTGATACCGCACAGATAAT13.125No Hit
CTCGCGATACACGTGACGACGCAAAAGGGAAAGGAAGATATTGACAGATG13.125No Hit
CGCGCGATACACGTGACGACGCAGGGGGGAAATAAAAACAACCAAAATGA13.125No Hit
CGTGCGATACACGTGACGACGCCCGAAAAACAATCTGGACTAGTGCGAGC13.125No Hit
CGCGCGATACACGTGACGACGCACGGGCAGAAATCATACTGAAAGCGAGC13.125No Hit
AGCGCGATACACGTGACGACGCAAGCAACGAAATGTCAATGAGAGGAGTG13.125No Hit
CGTGCGATACACGTGACGACGCTTTGCAGGGAAGAACACTGATCTTGAGG13.125No Hit
CGCGCGATACACGTGACGACGCCAGCGGCGGGGAGACACAGGACGACCCG13.125No Hit
CGCGCGATACACGTGACGACGCAACAGGGGAAAATAAAAACAACCAAAAT13.125No Hit
CCCGCGATACACGTGCCGACGCAAAAAAAGAAAAGCTGATCGCACTAGTA13.125No Hit
CGCGCGATACACGTGACGACGCCACCCCTTCTCCAATCTCATCGCGCTCT13.125No Hit
AGCGCGATACACGTGACGACGCAGGGGGGGAAGACATAATGGATCCAAAC13.125No Hit
CGCGCGATAAACGTGACGACGCGAGTAGCAAATGCACGGACAAACTAAAA13.125No Hit
CGCGCGATGCACGTGACGACGCACACAGGGAACAGAGAAACTGACAATAA13.125No Hit
CGCGCGATACCCGTGAAGACGCCTCGACTTTTAAGACATTTTCAGAAGGA13.125No Hit
CGTGCGATACACGTGACGACGCACACGGGAAATAGCAGAAAGACCCAAAG13.125No Hit
TGCGCGATACACGTGACGACGCATTATATGTCCAAATGTCCAGAAATCCA13.125No Hit
CGTGCGATACACGTGACGACGCCGGACCCAAAGCCTCTACTCAGTGCGAA13.125No Hit
CGCGCGATACACGTGACGACGCAACGCCTGGTCTGACTCACATGATGATC13.125No Hit
CGCGCGATACACGTGGCGAGGCCCGCAGCGAACTCGAACTCAGTGCTTAT13.125No Hit
CGCGCGATACACGTGACGACGCCGATCCAGCCATTTGCTCCATAGCCTTA13.125No Hit
CGCGCGATACACGTGACGACGCCAAGGGGCAATAAATACCTGGAAGAACA13.125No Hit
CGCGCGATACACGTGACGACGCAGACGATTGCCGAAGCAATAATTGTGGC13.125No Hit
CGCGCGATACACGTGACGACGCAAACGAGAAATGGCGGGAACAATTAGGG13.125No Hit
CGCGCGATACACGTGACGACGCAGCAGGGGAAAATAAAAACAACCAAAAT13.125No Hit
CGGGCGATACACGTGACGACGCAGAGCCGGAACCCTAGACAAGTGAGATC13.125No Hit
CGCGCGATACACGGGACGACGCCCACGGGTGATAAGTAGAAACAAAGTCG13.125No Hit
CGTGCGATACACGTGACGACGCAGAGGGAAAAGGGAGACCAAAAGCACCA13.125No Hit
CGTGCGATACACGTGACGACGCAAACCGAGAAGCATGAAGCAAAACCCGA13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)