FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC348CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC348CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC90.6756756756756757No Hit
CGTCGAGACACTACGCAGCGTCCTACGCTGCAGTCCTCGCTCACTGGGCA30.22522522522522523No Hit
CGTCGAGACACTACGCAGCGTCATACTCCATATTTTTGGCTGGACCAATT30.22522522522522523No Hit
CGTGGAGACACTACGCAGCGTCAGTAGAAACAAGGTCGTTTTTAAATTAT20.15015015015015015No Hit
CGTTGAGACACTACGCAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCAC20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCCGCAGACAAGCTGCAGACCAAGATCTAG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCATCAGCAAAAGCAGGTAGATATTGAAAG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGTAGTTTTTTACTCTA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGAGACACTACGCAGCGTCAGTAGAAACA20.15015015015015015No Hit
CGTAGAGACACTACGCAGCGTCATACTCCATATTTTTGGCTGGACCAATT20.15015015015015015No Hit
CGTTGAGACACTACGCAGCGTCAGGAGAAACAAGGTCGTTTTTAAATTAT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGGCCAGGGAAGTTGCTAGTCAGACCAGT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCGGGGGACTCGAACTGTGTTATCATTCCA20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAGTAGAAACAAGGTACTTTTTTGGACAG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCATCAGCGAAAGCAGGTAGATATTGAAAG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAGGAGAAACAAGGTCGTTTTTAAATTAT20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGGCTACGCTGCAGTCCTCGCTCACTGGGCAC20.15015015015015015No Hit
CGTTGAGACACTACGCAGCGTCAGTAGAAACAAGGCATTTTTTCATGAAG20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAGACCAGTGCTAGAGCTAGGATGAGTCC20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCATCACCATTGTTGGCTTGGCGCCAAATC20.15015015015015015No Hit
CGTCGAGACACTACGCAGCGTCAACGGGCGTCGAGACACTACGCAGCGGC20.15015015015015015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGAC800.095.000013
TGAGACA305.16593E-995.04
GTTGAGA305.16593E-995.02
ACACTAC1200.095.08
TTGAGAC305.16593E-995.03
GACACTA1200.095.07
CACTACG1200.095.09
CGAGACA850.095.04
CGTTGAG305.16593E-995.01
AGACACT1200.095.06
GAGACAC1150.094.999995
GTCGAGA900.084.444452
CGTCGAG800.083.1251
CGTCAGG204.4513764E-447.50000418-19
GCGTCGG204.4513764E-447.50000418-19
GCGTCAA204.4513764E-447.50000418-19
CGTCGGA204.4513764E-447.50000418-19
AGCGTCC204.4513764E-447.50000416-17
GCGTCAG309.941541E-747.518-19
CTACGCA1100.047.510-11
Produced by FastQC (version 0.11.5)