FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC346CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC346CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348247
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTATATACTCTAGCGAGTGGCCGAGACAAGACAAATCCTAAAATCCC43171.2396373838109158No Hit
CGTGTATATACTCTAGCGAGTGATTAGCAAAAGCAGGTAGATATTGAAAG39331.1293708201362824No Hit
CGTGTATATACTCTAGCGAGTGATTAGCGAAAGCAGGTAGATATTGAAAG33660.9665553472104569No Hit
CGTGTATATACTCTAGCGAGTGAACAGCAAAAGCAGGTAGATATTGAAAG32470.9323841985717033No Hit
CGTGTATATACTCTAGCGAGTGAACAGCGAAAGCAGGTAGATATTGAAAG31550.9059661676913225No Hit
CGTGTATATACTCTAGCGAGTGGCCAAGACAAGACAAATCCTAAAATCCC30460.8746665441482625No Hit
CGTGTATATACTCTAGCGAGTGGCAAGGACAAGACAAATCCTAAAATCCC29850.8571502410645317No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTGAAAG29280.8407825480190784No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCAAAAGCAGGTAGATATTGAAAG27650.7939766889592732No Hit
CGTGTATATACTCTAGCGAGTGATCAGCGAAAGCAGGTAGATATTGAAAG24740.7104153086745901No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCGAAAGCAGGTAGATATTGAAAG23910.6865816503803335No Hit
CGTGTATATACTCTAGCGAGTGATAAGCAAAAGCAGGTAGATATTGAAAG19450.5585116311124001No Hit
CGTGTATATACTCTAGCGAGTGGCGCAGACAAGACAAATCCTAAAATCCC19410.5573630210741227No Hit
CGTGTATATACTCTAGCGAGTGACCAGCAAAAGCAGGTAGATATTGAAAG18720.537549497913837No Hit
CGTGTATATACTCTAGCGAGTGGCACAGACAAGACAAATCCTAAAATCCC18700.5369751928946983No Hit
CGTGTATATACTCTAGCGAGTGCAGCGAAAGCAGGTAGATATTGAAAGAT18510.5315192952128805No Hit
CGTGTATATACTCTAGCGAGTGATAAGCGAAAGCAGGTAGATATTGAAAG18270.524627634983216No Hit
CGTGTATATACTCTAGCGAGTGACCAGCGAAAGCAGGTAGATATTGAAAG17790.5108443145238868No Hit
CGTGTATATACTCTAGCGAGTGATGAGCAAAAGCAGGTAGATATTGAAAG15940.4577211002535556No Hit
CGTGTATATACTCTAGCGAGTGGCGACGACAAGACAAATCCTAAAATCCC15080.4330259844305909No Hit
CGTGTATATACTCTAGCGAGTGATGAGCGAAAGCAGGTAGATATTGAAAG14710.42240134157652476No Hit
CGTGTATATACTCTAGCGAGTGCTCAGCAAAAGCAGGTAGATATTGAAAG14560.4180940539329843No Hit
CGTGTATATACTCTAGCGAGTGGCCCAGACAAGACAAATCCTAAAATCCC14250.4091923261363342No Hit
CGTGTATATACTCTAGCGAGTGGCAAAGGCAAGACAAATCCTAAAATCCC14110.40517219100236324No Hit
CGTGTATATACTCTAGCGAGTGGCCAGGACAAGACAAATCCTAAAATCCC13540.38880449795690986No Hit
CGTGTATATACTCTAGCGAGTGGCGAAGACAAGACAAATCCTAAAATCCC11810.33912711380141103No Hit
CGTGTATATACTCTAGCGAGTGCTCAGCGAAAGCAGGTAGATATTGAAAG11460.32907677596648355No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCGAAAGACAAGACAAATCCTAA10420.2992129149712704No Hit
CGTGTATATACTCTAGCGAGTGATCCGCAAAAGCAGGTAGATATTGAAAG9900.28428098447366384No Hit
CGTGTATATACTCTAGCGAGTGCAGCAAAAGCAGGTAGATATTGAAAGAT9890.28399383196409445No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCCAAAGACAAGACAAATCCTAA9850.282845221925817No Hit
CGTGTATATACTCTAGCGAGTGGCAACGACAAGACAAATCCTAAAATCCC9800.28140945937797024No Hit
CGTGTATATACTCTAGCGAGTGGGGGTGCAGATGCAACGATTCAAGTGAT9740.27968654432055406No Hit
CGTGTATATACTCTAGCGAGTGAAAGCAGGAAGATATTGAAAGATGAGTC9300.2670518338995024No Hit
CGTGTATATACTCTAGCGAGTGATCCGCGAAAGCAGGTAGATATTGAAAG9260.26590322386122495No Hit
CGTGTATATACTCTAGCGAGTGATCAACGAAAGCAGGTAGATATTGAAAG8460.24293102309567632No Hit
CGTGTATATACTCTAGCGAGTGGGGGGCTATATGAAGCAATTGAGGAGTG8400.2412081080382602No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCAAAAGACAAGACAAATCCTAA8300.23833658294256663No Hit
CGTGTATATACTCTAGCGAGTGATCAACAAAAGCAGGTAGATATTGAAAG7870.22598902503108426No Hit
CGTGTATATACTCTAGCGAGTGACAAGCAGCTGAGGCCATGGAGGTTGCT7620.21881021229185035No Hit
CGTGTATATACTCTAGCGAGTGGCCGGGACAAGACAAATCCTAAAATCCC7470.21450292464830997No Hit
CGTGTATATACTCTAGCGAGTGGCGCCGACAAGACAAATCCTAAAATCCC7450.21392861962917123No Hit
CGTGTATATACTCTAGCGAGTGCGGCAACGATTCAAGTGATCCTCTTGTT7340.21076994202390834No Hit
CGTGTATATACTCTAGCGAGTGATCATCAAAAGCAGGTAGATATTGAAAG7300.2096213319856309No Hit
CGTGTATATACTCTAGCGAGTGGCGGCGACAAGACAAATCCTAAAATCCC7290.20933417947606153No Hit
CGTGTATATACTCTAGCGAGTGGCAGAGACAAGACAAATCCTAAAATCCC7260.20847272194735345No Hit
CGTGTATATACTCTAGCGAGTGGCGGAGACAAGACAAATCCTAAAATCCC6610.18980780882534523No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCAAATGCAACGATTCAAGTGAT6600.18952065631577586No Hit
CGTGTATATACTCTAGCGAGTGATCATCGAAAGCAGGTAGATATTGAAAG6470.18578767369137422No Hit
CGTGTATATACTCTAGCGAGTGCGGAGGCCATGGTCATTTTAAGTGCCTC6250.17947031848084835No Hit
CGTGTATATACTCTAGCGAGTGGGGTGCAGATGCAACGATTCAAGTGATC5460.15678527022486913No Hit
CGTGTATATACTCTAGCGAGTGGAGGCGGCAACCTGTGAACAGATTGCCG5300.1521908300717594No Hit
CGTGTATATACTCTAGCGAGTGGCGAGGACAAGACAAATCCTAAAATCCC5010.14386340729424804No Hit
CGTGTATATACTCTAGCGAGTGAGAAGCGAAAGCAGGTAGATATTGAAAG4950.14214049223683192No Hit
CGTGTATATACTCTAGCGAGTGGCAAAGACAAGACAAATCCTAAAATCCC4610.13237730691147376No Hit
CGTGTATATACTCTAGCGAGTGGAGGGCAAAAGACAAGACAAATCCTAAA4540.13036723934448824No Hit
CGTGTATATACTCTAGCGAGTGCACCGAGCTTAAGCTCAATGATTATGAG4470.12835717177750275No Hit
CGTGTATATACTCTAGCGAGTGCCTAAAATGAGGAATCCCCTTAGAACCG4450.12778286675836403No Hit
CGTGTATATACTCTAGCGAGTGGAGAGGCAAAAGACAAGACAAATCCTAA4440.12749571424879466No Hit
CGTGTATATACTCTAGCGAGTGGAAAAGACAAGACAAATCCTAAAATCCC4330.12433703664353174No Hit
CGTGTATATACTCTAGCGAGTGAGAAGCGGAAGCAGGTAGATATTGAAAG4310.12376273162439304No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCTGAGGCCATGGAGGTTGCTAGT4240.12175266405740753No Hit
CGTGTATATACTCTAGCGAGTGAAGCGAAAGCAGGTAGATATTGAAAGAT4170.11974259649042203No Hit
CGTGTATATACTCTAGCGAGTGGCCAAGGCAAGACAAATCCTAAAATCCC4100.11773252892343652No Hit
CGTGTATATACTCTAGCGAGTGAAAAGCAAAAGCAGGTAGATATTGAAAG4070.11687107139472847No Hit
CGTGTATATACTCTAGCGAGTGAAAGCAGGTAGATATTGAAAGATGAGTC4050.11629676637558975No Hit
CGTGTATATACTCTAGCGAGTGAGGCCCCCTCAAAGCCGAGATCGCACAG4010.1151481563373123No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCAAAGACAAGACAAATCCTAAA3970.11399954629903487No Hit
CGTGTATATACTCTAGCGAGTGAACAGGGAAAGCAGGTAGATATTGAAAG3940.1131380887703268No Hit
CGTGTATATACTCTAGCGAGTGAGAAAGCGAAAGCAGGTAGATATTGAAA3900.11198947873204937No Hit
CGTGTATATACTCTAGCGAGTGAGAAGCAAAAGCAGGTAGATATTGAAAG3890.11170232622248003No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCAAATGCAACAACAAGAGGATC3840.11026656367463324No Hit
CGTGTATATACTCTAGCGAGTGAACAGCGGAAGCAGGTAGATATTGAAAG3820.10969225865549452No Hit
CGTGTATATACTCTAGCGAGTGATGAGAGGAGAAATAAATATCTAGAGGA3800.1091179536363558No Hit
CGTGTATATACTCTAGCGAGTGGGGGGCTATAAGAAGCATTAAGCAAAAC3770.10825649610764773No Hit
CGTGTATATACTCTAGCGAGTGGAGGGCGAAAGACAAGACAAATCCTAAA3750.10768219108850902No Hit
CGTGTATATACTCTAGCGAGTGGAGAGGCGAAAGACAAGACAAATCCTAA3720.10682073355980096No Hit
CGTGTATATACTCTAGCGAGTGAAAAGCGAAAGCAGGTAGATATTGAAAG3700.10624642854066223No Hit
CGTGTATATACTCTAGCGAGTGACCAGCGAAAGCAGGTCAATTATATTCA3600.10337490344496866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT850.095.091851
CGAGTAT254.587364E-795.091851
CGTCTAT201.7337743E-595.091851
CGTGTAC254.587364E-795.091851
CGTGTAA353.274181E-1095.091851
CGCGTAT201.7337743E-595.091851
CGTTTAT409.094947E-1295.091851
CGTGTAG301.2212695E-895.091841
TACCCTA353.2923708E-1094.996279
GTACATA254.6149398E-794.996274
TACGCTA409.094947E-1294.996279
ATATACC353.2923708E-1094.996276
GTATCTA353.2923708E-1094.996274
TACTCTG1100.094.996279
TATAGTC500.094.996277
GTATAAA650.094.996274
ATAGTCT500.094.996278
ATAAACT650.094.996276
ATACGCT409.094947E-1294.996278
TATACCC353.2923708E-1094.996277
Produced by FastQC (version 0.11.5)