FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC345CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC345CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41420
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT4351.0502172863351038No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTATTTTTCATTAAT2580.6228874939642685No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCTA2150.5190729116368904No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTCGTTTTTAAATTAT1670.40318686624818934No Hit
CGAGACGTAGATGACGAGTATCGGGGCGGGAACAACTAGGTCAGAAGTTT1360.32834379526798646No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGTGTTTTTTATCACT1290.31144374698213423No Hit
CGAGACGTAGATGACGAGTATCCGGGCGGGAACAACTAGGTCAGAAGTTT1240.2993722839208112No Hit
CGAGACGTAGATGACGAGTATCCAGGCGGGAACAACTAGGTCAGAAGTTT1170.28247223563495893No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTCCTTATA1040.2510864316755191No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGGTATTTTTCATTAAT970.23418638338966682No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGTAGTTTTTTACTCTA860.20762916465475617No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTACTTTTTTGGACAG810.1955577015934331No Hit
CGAGACGTAGATGACGAGTATCGAAAGGGGAAAAAGCTAATGTGCTAATT650.15692901979719942No Hit
CGAGACGTAGATGACGAGTATCCCGCGCTGGGATGTTCCTCTAGATATTT620.1496861419604056No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGGGTTTTTTATCACT620.1496861419604056No Hit
CGAGACGTAGATGACGAGTATCGCGGGGACAACTAGGTCAGAAGTTTGAA590.14244326412361177No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGTCGTTTTTAAATTAT570.13761467889908258No Hit
CAAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT560.13520038628681796No Hit
CGAGACGTAGATGACGAGTATCGGGGGCAGAAATCATAAAGATGATGGAA540.13037180106228874No Hit
CGAGACGTAGATGACGAGTATCGAGGGCAGAAGTTTGAAGAAGTAAGATG540.13037180106228874No Hit
CGAGACGTAGATGACGAGTATCAGCCGCCGAGACGTAGATGACGAGTATC500.12071463061323032No Hit
CGAGACGTAGATGACGAGTATCAAGGCGGGAACAACTAGGTCAGAAGTTT470.11347175277643651No Hit
CGGGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT450.10864316755190728No Hit
CGAGACGTAGATGACGAGTATCGGGGGGGCAATATGAAGCAATTGAGGAG450.10864316755190728No Hit
CGAGATGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT450.10864316755190728No Hit
CGAGACGTAGATGACGAGTATCCAGGGAACAACTAGGTCAGAAGTTTGAA440.10622887493964268No Hit
CGAGACGTAGATGACGAGTATCGAGGCGGGAACAACTAGGTCAGAAGTTT440.10622887493964268No Hit
CGAGACGTAGATGACGAGTATCAGTAGCAACAAGGTACTTTTTTGGACAG430.10381458232737809No Hit
CGAGACGTAGATGACGAGTATCATGCGCCGAGACGTAGATGACGAGTATC420.10140028971511349No Hit
CGAGACGTAGATGATGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT420.10140028971511349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACG156.541341E-495.113661
CGAGACT301.19271135E-895.113661
GGATGAC201.7240674E-594.9987959
AACGTAG201.7240674E-594.9987954
GCGACGT156.5728946E-494.9987952
TAGCTGA156.5728946E-494.9987958
AGATTAC500.094.9987959
TAGATTA500.094.9987958
AGCTGAC156.5728946E-494.9987959
ACGTGGA254.542253E-794.9987955
CGAGATG1800.092.471611
GAGATGT1800.092.359932
CGGGACG1100.090.790311
GGACGTA1100.090.6806643
ATGTAGA1850.089.8637165
TAGATGA40550.089.376188
ACGTAGA38600.089.338245
CGTAGAT38800.089.122586
GACGTAG38800.089.000164
GTAGATG40900.088.611347
Produced by FastQC (version 0.11.5)