FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC337CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC337CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152401
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGAGTAGTACAGTGTCTGAAACGAAAACGTTCTAATCTCTTGTTCCA10500.6889718571400449No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGCGAAGACATAATGGATTCCCAC4360.28608736163148535No Hit
CGTAGAGTAGTACAGTGTCTGACGGGGTACAAACCATTCTTCCCCGTCAG3930.25787232367241686No Hit
CGTAGAGTAGTACAGTGTCTGAAGAAGAATCCGATGAGGCACTTAAAATG3840.25196685061121643No Hit
CGTAGAGTAGTACAGTGTCTGAAAAAGCAATGAAAGAGTATGGAGAGGAC3790.24868603224388292No Hit
CGTAGAGTAGTACAGTGTCTGAAAGCGCATGCCATGATGGCATGGGCTGG3600.23621892244801543No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGCGGGAACAACTAGGTCAGAAGTTT3450.2263764673460148No Hit
CGTAGAGTAGTACAGTGTCTGAATGAGCGGGTCATTATGGTGAAACATGA3010.19750526571347954No Hit
CGTAGAGTAGTACAGTGTCTGACAGCGACCAAATTAATTGATGGCCATCC2680.17585186448907816No Hit
CGTAGAGTAGTACAGTGTCTGATTGAGGAAGTGAGGCATAAATGTTATTT2540.16666557306054422No Hit
CGTAGAGTAGTACAGTGTCTGATTGGTCAGGATGTTGGACGAAACTTCCG2480.16272859101974396No Hit
CGTAGAGTAGTACAGTGTCTGAAAGGGGTTTGAGATGATTTGGGATCCTA2460.16141626367281056No Hit
CGTAGAGTAGTACAGTGTCTGACCTAGTTGTTCCCGCCATTTTCCGTTTC2340.15354229959121002No Hit
CGTAGAGTAGTACAGTGTCTGAAGAAACAAGGTCGTTTTTAAATTATTCG2240.14698066285654293No Hit
CGTAGAGTAGTACAGTGTCTGACCGAGAGTGCTGCCTCTTCCCTTTAGAG2230.14632449918307622No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGCAGGAATGAGAATACTGGAGAATT2210.1450121718361428No Hit
CGTAGAGTAGTACAGTGTCTGAAGGGCGGGAACAACTAGGTCAGAAGTTT2190.1436998444892094No Hit
CGTAGAGTAGTACAGTGTCTGATGGCCATCCGAATTCTTTTGGTCGCTGT2160.14173135346880925No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGCAAAGACATAATGGATTCCCAC2140.14041902612187585No Hit
CGTAGAGTAGTACAGTGTCTGAGGTCTTCAATGGTGGAACAGATCTTCAT2100.13779437142800902No Hit
CGTAGAGTAGTACAGTGTCTGAAAACCCTTTTACTCCGTTTGCTCCATCA2000.1312327346933419No Hit
CGTAGAGTAGTACAGTGTCTGAGGAAGGGGAAAAAGCTAATGTGCTAATT1890.1240149342852081No Hit
CGTAGAGTAGTACAGTGTCTGACGCCACAAAAAGAAATGCTGCTCCCACT1850.12139027959134127No Hit
CGTAGAGTAGTACAGTGTCTGACCCAGCTATCTCAATGCATGTGTTAGGA1850.12139027959134127No Hit
CGTAGAGTAGTACAGTGTCTGACTTAGCCAACAGTGTCCTGCATACTTTC1840.12073411591787456No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGCTATATGAAGCAATTGAGGAGTG1760.11548480653014088No Hit
CGTAGAGTAGTACAGTGTCTGACGGGCGGGAACAACTAGGTCAGAAGTTT1750.11482864285667417No Hit
CGTAGAGTAGTACAGTGTCTGAATCAAAATGGAAGATTTTGTACGACAAT1730.11351631550974076No Hit
CGTAGAGTAGTACAGTGTCTGAAGGCCCTCTTTTTAAACCGTGTTTAAAG1630.10695467877507366No Hit
CGTAGAGTAGTACAGTGTCTGAATGAGCTACTTGGGAAGAATTTTTCAAA1620.10629851510160694No Hit
CGTAGAGTAGTACAGTGTCTGAGGGGGGGCAAAGACATAATGGATTCCCA1540.10104920571387327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG450.095.123531
CGTAGCG353.237801E-1095.123521
ATTAGTA201.7390776E-594.9986956
TAGATTA201.7390776E-594.9986953
TAGTAGT201.7390776E-594.9986955
AGCGTAG353.274181E-1094.998694
TAGTTCA156.607183E-494.998698
CGTAGTA353.274181E-1094.998696
AGTAGTC450.094.998696
AGTACCG750.094.998699
GTAGTTC156.607183E-494.998697
GTAGTAG301.2249075E-894.998697
AGTAAAG750.094.998699
TAGTACC750.094.998698
TAGTAAA750.094.998698
AGTACAG145500.093.8560949
TAGTACA142550.093.665848
CGTAGAG149700.093.6302641
GAGTAGT145100.093.558325
GTAGTAC143900.093.546317
Produced by FastQC (version 0.11.5)