FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC333CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC333CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACGACTACGCGAGACACCCAAGATCAAAGGTCCCAGGTTCCAGAT30.24590163934426232No Hit
CGACGACGACTACGCGAGACACATGACCCGACGACGACTACGCGAGACAC30.24590163934426232No Hit
CGACGACGACTACGCGAGACACCGACGACGACGACGACTACGCGAGACAC20.16393442622950818No Hit
CGACGACGACTACGCGAGACACATTCCATGGGGCCAAAGAAATAGCACTC20.16393442622950818No Hit
CGATGACGACTACGCGAGACACCAAAGAGCAAAGGTCCCAGGCTCTTAGG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACCTCAGCCGACGACGACTACGCGAGACAC20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAGTCACTGGATTGCAGCTGCCCTCTCCA20.16393442622950818No Hit
CGAGGACGACTACGCGAGACACCAAGGACCAAAAGTAACAGACTTAGAAA20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGGGACCAAAAGTAACAGACTTAGAAAGG20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGCATGGCGACGACGACTACGCGAGACAC20.16393442622950818No Hit
CGACGACGACTACGCGAGACACAAGCACCGACGACGACTACGCGAGACAC20.16393442622950818No Hit
CGACGACGACTACGCGAGACACACGCTCGACGACGACTACGCGAGACACA20.16393442622950818No Hit
CGACGACGACTACGCGAGACACGGGAAACGAAAACGTTCTAATCTCTTGT20.16393442622950818No Hit
CGACGACGACTACGCGAGACACTCAGGGCGACGACGACTACGCGAGACAC20.16393442622950818No Hit
CGACGACGACTACGCGAGACACCAACACTACATCCCCTTGCCCAATTAGC20.16393442622950818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT1300.080.384625
ACGACTA1300.080.384626
ACTACGC1300.080.384629
GACTACG1300.080.384628
CGACTAC1250.079.7999957
GACGACG1250.068.3999942
ACGACGA1250.068.3999943
ACACGGG150.00912341947.49999618-19
GACACGG204.3785325E-447.49999618-19
GACACAG150.00912341947.49999618-19
CCCGACG204.3785325E-447.49999626-27
CTACGCG1250.041.810-11
TACGCGA1150.041.30434810-11
ACGCGAG1250.039.89999812-13
CGAGACA1200.039.5833314-15
GCGAGAC1200.039.5833314-15
CGCGAGA1150.039.23913212-13
AGACACA501.4146281E-838.016-17
GAGACAC1300.036.53846416-17
CGACGAC2400.035.6249961
Produced by FastQC (version 0.11.5)