FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC331CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC331CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4556
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACTCTCGCATAGACTACTAGGGGGGGGAAGTGAGGAATGATGATGTT350.7682177348551361No Hit
CGTACTCTCGCATAGACTACTAAGGCCCTCCTTTCAGTCCGTATTTAAAG240.5267778753292361No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGCCTATCA230.504828797190518No Hit
CGTACTCTCGCATAGACTACTACGGCACTCCTTCCGTAGAAGGCCCTCCT170.3731343283582089No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGGTGTTT160.35118525021949076No Hit
CGTACTCTCGCATAGACTACTAAGGCACTCCTTCCGTAGAAGGCCCTCCT160.35118525021949076No Hit
CGTACTCTCGCATAGACTACTAGGGGGCCAAATCAGCATACAACCTACGT150.3292361720807726No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGTAGTTT150.3292361720807726No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGGAAGGGAGGAATGATGATGTT110.2414398595258999No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTCTATCA100.21949078138718175No Hit
CGTACTCTCGCATAGACTACTACAGCTCTATGCTGACAAAATGACCATCG100.21949078138718175No Hit
CGTACTCTCGCATAGACTACTAGGGGCCGCAAATATCATTGGGATCTTGC90.19754170324846357No Hit
CGTACTCTCGCATAGACTACTAAGGGGGGGAAGTGAGGAATGATGATGTT80.17559262510974538No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGGAACAATTAGGTCAGAAGTTT80.17559262510974538No Hit
CGTACTCTCGCATAGACTACTAGGGGCGGGAACAATTAGGTCAGAAGTTT70.15364354697102722No Hit
CGTACTCTCGCATAGACTACTACGGCGCTCCTTCCGTAGAAGGCCCTCCT70.15364354697102722No Hit
CGTACTCTCGCATAGACTACTAGGGCGGGAACAATTAGGTCAGAAGTTTG60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTAAGGGCGGGAACAATTAGGTCAGAAGTTT60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGCAAATCAGCATACAACCTACG60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGAAGTGAGGAATGATGATGTTG60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTACGGGGGGGAAGTGAGGAATGATGATGTT60.13169446883230904No Hit
CGTACTCTCGCATAGACTACTAGGCCGCAAATATCATTGGGATCTTGCAC50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAGGGCACTCCTTCCGTAGAAGGCCCTCCT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAGGGGGGGGGAGTGAGGAATGATGATGTT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAAGCCGCAAATATCATTGGGATCTTGCAC50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTAGGGAGGGGAAGTGAGGAATGATGATGTT50.10974539069359088No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGTTGTTT50.10974539069359088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA4400.093.9204568
CGCATAG4400.093.9204569
CTCGCAT4400.093.9204567
TCTCGCA4350.093.908056
CGTACTC4450.091.797751
GTACTCT4400.091.761372
TACTCTC4400.091.761373
ACTCTCG4450.090.730344
CTCTCGC4650.086.827965
AGGGGGG451.7826096E-1047.50000422-23
TCCTTCC252.61047E-547.528-29
ACTAAGG700.047.518-19
CTCCTTC252.61047E-547.528-29
CTAAGGC356.823575E-847.520-21
CCTTCCG252.61047E-547.530-31
CTACGCG301.3345816E-647.520-21
TAGGGGG600.047.520-21
CTACTAC1350.047.516-17
CTTCCGT252.61047E-547.530-31
TACTACA252.61047E-547.518-19
Produced by FastQC (version 0.11.5)