FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC330CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC330CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8550
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATATAGTACTGTCGCTGGGGGGGGAAAGATCCTAAGAAAACTGGA470.5497076023391813No Hit
CGAGTATATAGTACTGTCGCTGGGGGGCTATATGAAGCAATTGAGGAGTG440.5146198830409356No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAAGATCCTAAGAAAACTGG340.39766081871345027No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGTGAGGAATGATGATGTT300.3508771929824561No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAATGGACGAAAAACAAGAAT250.29239766081871343No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAACAATTAGGTCAGAAGTTT200.23391812865497078No Hit
CGAGTATATAGTACTGTCGCTGGGGGGAACAATTAGGTCAGAAGTTTGAA170.19883040935672514No Hit
CGAGTATATAGTACTGTCGCTGAGGAGCTGAGGGAGCAATTGAGCTCAGT150.17543859649122806No Hit
CGAGTATATAGTACTGTCGCTGGGGGCGGGAACAATTAGGTCAGAAGTTT130.15204678362573099No Hit
CGAGTATATAGTACTGTCGCTGAAACCATAAAAAGTTGGAGGAAGAAAAT120.14035087719298245No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGAGAATGGACGAAAAACAAGAAT120.14035087719298245No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGAAAATATTGAGGACACA110.1286549707602339No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGAAAATAAAAACAACCAAAATG110.1286549707602339No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGAAAATAAAAACAACCAAAATGA100.11695906432748539No Hit
CGAGTATATAGTACTGTCGCTGGGGGCCTATACATATTGTGTCTGCATCT100.11695906432748539No Hit
CGAGTATATAGTACTGTCGCTGGGAGGGGAAAGATCCTAAGAAAACTGGA100.11695906432748539No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGCAATTAGGTCAGAAGTTTGAA100.11695906432748539No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGATCCTAAGAAAACTGGA90.10526315789473684No Hit
CGAGTATATAGTACTGTCGCTGAAGATAACAGAGAATAGTTTTGAGCAAA90.10526315789473684No Hit
CGAGTATATAGTACTGTCGCTGGGGGGAGAAAATATTGAGGACACAAGAG90.10526315789473684No Hit
CGAGTATATAGTACTGTCGCTGGGGGAAGCAGTAATGAGAATGGGAGACC90.10526315789473684No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGTGAGAATGGACGAAAAACAAGA90.10526315789473684No Hit
CGAGTATATAGTACTGTCGCTGGGGAGGGGAAGTGAGGAATGATGATGTT90.10526315789473684No Hit
CGAGTATATAGTACTGTCGCTGGGGGGGGGAAGGGAGGAATGATGATGTT90.10526315789473684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT8200.095.09
AGTATAT8100.094.999993
GAGTATA8100.094.999992
ATAGTAC8100.094.999998
ATATAGT8300.094.427716
CGAGTAT8200.094.420741
TATATAG8150.094.417185
GTATATA8150.094.417184
TATAGTA8350.093.862287
CGCTGCT301.4072448E-647.518-19
CGCTGCG1000.047.518-19
CGCTGAT501.0913936E-1147.518-19
CGCTGAG1100.047.518-19
CGCTGAC252.7109929E-547.518-19
GGAGAAA252.7109929E-547.526-27
GATGTTG252.7109929E-547.544-45
CTGCGGG252.7109929E-547.520-21
GCTGCGG550.047.518-19
TCGCTGA2250.047.516-17
GCTGAGC252.7109929E-547.518-19
Produced by FastQC (version 0.11.5)