FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC324CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC324CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10362
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCAGTCTCTATACGTCTAGCGGGGGGGGAAAATGGACGAAGGACAAGG360.3474232773595831No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGAAAATGGACGAAGGACAAGGG320.30882069098629605No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGGGAACAGTGACCACAGAAGCTG310.2991700443929743No Hit
CGTCAGTCTCTATACGTCTAGCCGGCGCCATTCTGTTTTCATGCCTGATT170.1640609920864698No Hit
CGTCAGTCTCTATACGTCTAGCCGGGACAGAAATTTGAGGAAATAAGGTG170.1640609920864698No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGGCCAAGGAGGTGTCACTAAGCTA160.15441034549314803No Hit
CGTCAGTCTCTATACGTCTAGCGAGGGGGGAAAATGGACGAAGGACAAGG150.1447596988998263No Hit
CGTCAGTCTCTATACGTCTAGCGGGGGTTTTGTTCAGCATCCAGAACTAA130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCAGGGGGGGAACAGTGACCACAGAAGCTG130.12545840571318279No Hit
CGTCAGTCTCTATACGTCTAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA110.10615711252653928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTC201.6631146E-595.000014
CGTCAGT8350.095.01
CAGTCTT156.432572E-495.04
CTTTATA156.432572E-495.08
CGTTAGT156.432572E-495.01
TCAGGCT254.3032378E-795.03
TGTCAGT156.432572E-495.01
CTCTATA9400.095.08
CATCAGT156.432572E-495.01
TCTATAC9300.095.09
TCAGTCC156.432572E-495.03
GTCTCTA9050.095.06
GTTAGTC156.432572E-495.02
CGTCGGT301.1150405E-895.01
CAGGCTC254.3032378E-795.04
AGTCTTT156.432572E-495.05
CGGTCTC254.3032378E-795.04
GCTCTAT254.3032378E-795.07
AGTCCCT156.432572E-495.05
CGGCAGT156.432572E-495.01
Produced by FastQC (version 0.11.5)