FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC317CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC317CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4597
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGATATAGTCAGAGAGTAGGGGGGACAGAAATTTGAGGAAATAAGGT100.21753317380900586No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGGAAAATGGACGAAGGACAAGG100.21753317380900586No Hit
CGTCGATATAGTCAGAGAGTAGCTGAAACGAGAAAGCTCTTATCTCTTGT100.21753317380900586No Hit
CGTCGATATAGTCAGAGAGTAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA90.1957798564281053No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCAAAAACATAATGGACTCCAACA80.1740265390472047No Hit
CGTCGATATAGTCAGAGAGTAGGGGGCCTGACGGGATGATAGAAAGAACG70.1522732216663041No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCGGAAATTTGAGGAAATAAGGTG50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGCTGAGCTTTGGGTATGAATTTCCTTTTT50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGAAAGCTGGAATCAACAAGGAT50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCCAAAGTTCTTTCATTTTCCAAT50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGCAGAAATTTGAGGAAATAAGGTG50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGAAAATGGACGAAGGACAAGGG50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGCAAAAACATAATGGACTCCAAC50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGAGCAATTGGGACAGAAATTTGAG50.10876658690450293No Hit
CGTCGATATAGTCAGAGAGTAGGGGGGGGGAAATTTGAGGAAATAAGGTG50.10876658690450293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATA4150.095.04
TCGATAT4150.095.03
GTCGATA3950.095.02
TAGTCAG4150.095.09
ATAGTCA4150.095.08
CGTCGAT3950.095.01
ATCGATA156.198759E-495.02
ATATAGT4300.095.06
CATCGAT156.198759E-495.01
TAGTTAG201.5631202E-594.999999
ATAGTTA201.5631202E-594.999998
TATAGTT201.5631202E-594.999997
TATAGTC4250.094.999997
GATATAG4350.093.908055
AGTAGAG451.7826096E-1047.50000418-19
GAGTAGG1700.047.50000416-17
GAGTAGA850.047.50000416-17
GTAGCGG451.7826096E-1047.50000418-19
TAGCGGC301.3345816E-647.520-21
AGTAGGG950.047.518-19
Produced by FastQC (version 0.11.5)