FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC316CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC316CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACTCACGCGCGACACGAGGCCAAGGGTGTTGCCTCTTCCTTTTA30.2626970227670753No Hit
CGACATACTCACGCGCGACACGATTCTAATGTGAAGAACTTGTATGAAAA20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAGATGGCGACATACTCACGCGCGACACG20.17513134851138354No Hit
CGACATACTCACGCGCGACACGGGGGCAGAAATTTGAGGAAATAAGGGGG20.17513134851138354No Hit
CGACATACTCACGCGCGACACGATCAGCAAAAGCAGGGTGACAAAAACAT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAAGAAATAAGACCCTTCATTACTCATGT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAACAGAGGTTATAAGAATGATGGAAAGT20.17513134851138354No Hit
CGACATACTCACGCGCGACACGCGGCACAGATTGAAAGCGACAGAGAATA20.17513134851138354No Hit
CGACATACTCACGCGCGACACGAGAAAAGTTTCGAACAAATAACATTTAT20.17513134851138354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATA900.093.949731
TACTCAC1100.090.638436
ATACTCA1050.090.43295
GACATAC1000.090.206822
ACATACT1000.090.206823
CATACTC1000.090.206824
ACTCACG1150.086.6976247
TCACGCG1100.086.322319
CTCACGC1100.086.322318
ACACGAG150.00903531947.47727218-19
CACGAGG150.00903531947.47727218-19
GACACGC204.303643E-447.47727216-17
GACACGA309.224368E-747.47727216-17
ACGAGGC150.00903531947.47727220-21
CGCGACA1000.045.1034114-15
CGACACG1000.045.1034116-17
GCGACAC1000.045.1034114-15
GCGCGAC1000.045.1034112-13
CGCGCGA950.044.9784712-13
GACACGG455.18412E-942.20202316-17
Produced by FastQC (version 0.11.5)