FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC313CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC313CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19422
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAAATGGACGAAGGACAAGG1170.6024096385542169No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAGGCTCTATTGGGAAAGTG840.4324992276799506No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAAGGAGTTGAACCAAGATGC540.27803521779425394No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGAAAATGGACGAAGGACAAGGG540.27803521779425394No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAATAGTTGGAGAAATTTCAC420.2162496138399753No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCTATATGAAGCAATCGAGGAGTG410.21110081351045207No Hit
CGATAGTCGTACTCAGTGATAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA390.2008032128514056No Hit
CGATAGTCGTACTCAGTGATAGCGGCACAGATTGAAAGCGACAGAGAATA300.15446400988569664No Hit
CGATAGTCGTACTCAGTGATAGGAGGGGGGAAAATGGACGAAGGACAAGG290.14931520955617342No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAAAAACATAATGGACTCCAACA250.12872000823808052No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGCGATAGTCGTACTCAGTGATAG240.1235712079085573No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAAAATTAAACAGAGAAGAAATA230.11842240757903408No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGCAAAAACATAATGGACTCCAAC230.11842240757903408No Hit
CGATAGTCGTACTCAGTGATAGAGGCCCATTAGAGCACATCCAGAAACTG230.11842240757903408No Hit
CGATAGTCGTACTCAGTGATAGAGGCCAAGGGGGTTGCCTCTTCCTTTTA230.11842240757903408No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCTGGTGTTTATAGCACCCTTGGG230.11842240757903408No Hit
CGATAGTCGTACTCAGTGATAGGGGGGCAGAAATTTGAGGAAATAAGGTG220.11327360724951087No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGGAATAGTTGGAGAAATTTCAC210.10812480691998765No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAAAAACATAATGGACTCCAA210.10812480691998765No Hit
CGATAGTCGTACTCAGTGATAGCGGCGCAGATTGAAAGCGACAGAGAATA200.10297600659046442No Hit
CGATAGTCGTACTCAGTGATAGGGGGGGGCAATTGGGACAGAAATTTGAG200.10297600659046442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC201.700782E-595.000018
TCGTAAT201.700782E-595.000017
GTAGTAC201.700782E-595.000016
CGATAGA156.5194326E-495.01
CGTTAGT156.5194326E-495.01
TTGTACT301.1688826E-895.07
GTACTCC156.5194326E-495.09
ACGTACT156.5194326E-495.07
CGGACTC156.5194326E-495.08
AGACGTA156.5194326E-495.05
GACGTAC156.5194326E-495.06
AGTCGGA156.5194326E-495.05
TCGTCCT156.5194326E-495.07
GCGTACT156.5194326E-495.07
CGTCCTC156.5194326E-495.08
TAGACGT156.5194326E-495.04
GTTGTAC301.1688826E-895.06
GTCGGAC156.5194326E-495.06
GTACTCA18300.093.183059
CGTACTC18100.093.162998
Produced by FastQC (version 0.11.5)