FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC309CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC309CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11670
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAATGGACGAAGGACAAGG1351.1568123393316194No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAGTCTTCGAGCTCTCGGAC1070.9168808911739503No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGCAAAAACATAATGGACTCCAAC850.7283633247643531No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTGTTT740.6341045415595544No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAAAAACATAATGGACTCCAA640.5484147386461011No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGACAAAAACATAATGGACTCCAA500.42844901456726653No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGAAAATGGACGAAGGACAAGGGT470.40274207369323056No Hit
CGTAGTATCTATAGTACTGCTAAGCCCATACACACAAGCAGGCAGGCAGG410.3513281919451585No Hit
CGTAGTATCTATAGTACTGCTAACGCGAAGTAGGTACAGATGCAATTGTC380.32562125107112255No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAACAAAAGCAACAAAAAT380.32562125107112255No Hit
CGTAGTATCTATAGTACTGCTAGGGGGACAAAAACATAATGGACTCCAAC370.3170522707797772No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGAAAATGGACGAAGGACAAGGG310.2656383890317052No Hit
CGTAGTATCTATAGTACTGCTAGGGGACAGAAATTTGAGGAAATAAGGTG300.2570694087403599No Hit
CGTAGTATCTATAGTACTGCTAGAGGGGGGAAAATGGACGAAGGACAAGG290.24850042844901457No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGTAGATAT290.24850042844901457No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCAAAAACATAATGGACTCCAACA260.22279348757497855No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGGTGACAA250.21422450728363326No Hit
CGTAGTATCTATAGTACTGCTAGGGGGACGAAAACATAATGGACTCCAAC250.21422450728363326No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCAGAAATTTGAGGAAATAAGGTG240.2056555269922879No Hit
CGTAGTATCTATAGTACTGCTAGCGGGGGAAAACAAAAGCAACAAAAATG230.19708654670094258No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGAAAACAAAAGCAACAAAAATG230.19708654670094258No Hit
CGTAGTATCTATAGTACTGCTACCGCGAAGTAGGTACAGATGCAATTGTC230.19708654670094258No Hit
CGTAGTATCTATAGTACTGCTACAGAGCAGAAATGAAAAGTGGCGAGAGC220.18851756640959727No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGGGAAAAC220.18851756640959727No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGAAATGGACGAAGGACAAGGGT210.17994858611825193No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAATAGTTGGAGAAATTTCAC210.17994858611825193No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAACAGTGACCACAGAAGCTG180.15424164524421594No Hit
CGTAGTATCTATAGTACTGCTACCTCCAGAGCAGAAATGAAAAGTGGCGA180.15424164524421594No Hit
CGTAGTATCTATAGTACTGCTAGGCGGGGGAAAACAAAAGCAACAAAAAT180.15424164524421594No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGTCGTTT170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTAGGGGGCCAAAAACATAATGGACTCCAAC170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTACGCCCATACACACAAGCAGGCAGGCAGG170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAATCAGTTTCTGGATGTGCT170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTAGGGGCGGGAAGTCTTCGAGCTCTCGGAC170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTACCCCTCTTAGTTTGCATAGTTTCCCGTT170.1456726649528706No Hit
CGTAGTATCTATAGTACTGCTAGGCGAGGCAATTGGGACAGAAATTTGAG160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTAAGCGGGGGAAAACAAAAGCAACAAAAAT160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTATTGGGACAGAAATTTGAGGAAATAAGGT160.13710368466152528No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAACAAAAGCAACAAAAATG150.12853470437017994No Hit
CGTAGTATCTATAGTACTGCTAGAAGGTTAACAGAGTGTGTTACTGTTAC150.12853470437017994No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAATGGACGAAGGACAAGGG150.12853470437017994No Hit
CGTAGTATCTATAGTACTGCTAGCGAGGGCAATTGGGACAGAAATTTGAG140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTAGGCAGGGGAAAACAAAAGCAACAAAAAT140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTATTT140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTAAGGGGGACAAAAACATAATGGACTCCAA140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTAAGGGACAGAAATTTGAGGAAATAAGGTG140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGTAGTTT140.11996572407883462No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGCAAAAGCAGGAGTTTAA130.11139674378748927No Hit
CGTAGTATCTATAGTACTGCTATGCGGCACAGATTGAAAGCGACAGAGAA130.11139674378748927No Hit
CGTAGTATCTATAGTACTGCTAGGGCGACAAAAACATAATGGACTCCAAC120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAGGAGGGGGAAAATGGACGAAGGACAAGG120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTACGGGGGGAAAATGGACGAAGGACAAGGG120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTACGGGACAGAAATTTGAGGAAATAAGGTG120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAGGGACAGAAATTTGAGGAAATAAGGTGG120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAGGCGAGAGAAATTGGGACAGAAATTTGA120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAAGGGGGGCAAAAACATAATGGACTCCAA120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAAGGCGAAGTAGGTACAGATGCAATTGTC120.10282776349614395No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGCAAGAGAAGAACCCCGCACTC120.10282776349614395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTA11550.095.4051741
CTATAGT11500.094.995719
GTATCTA11550.094.995715
AGTATCT11500.094.995714
TATCTAT11550.094.995716
TCTATAG11450.094.9957058
ATCTATA11550.094.584477
GTAGTAT11700.093.777812
TAGTATC11650.093.772593
CGCTGGA301.4302786E-647.49785664-65
CTTTCAA301.4302786E-647.49785676-77
TGGTCTA205.2807474E-447.49785654-55
GATGGCA205.2807474E-447.49785664-65
CAAGATC301.4302786E-647.49785680-81
TAGCGGA205.2807474E-447.49785620-21
AGGGGAG205.2807474E-447.49785622-23
CCAATTA205.2807474E-447.49785680-81
AAGTAGG205.2807474E-447.49785628-29
TTCACAG205.2807474E-447.49785690-91
CAGCATC205.2807474E-447.49785694-95
Produced by FastQC (version 0.11.5)