FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC307CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC307CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7894
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACAGAGTAGTACAGACAGGCCCCCTCAAAGCCGAGATCGCGCAG190.24068913098555864No Hit
CGACATACAGAGTAGTACAGACGGGGGGGGAACGCCAGGATGCCACAGAA170.21535343298707882No Hit
CGACATACAGAGTAGTACAGACGGGGGGAAAATGGACGAAGGACAAGGGT100.12667848999239928No Hit
CGACATACAGAGTAGTACAGACCGGCGCTGGAGCTAGGATGAGTCCCAAT100.12667848999239928No Hit
CGACATACAGAGTAGTACAGACGGGGGGGGAACAGTGACCACAGAAGCTG90.11401064099315936No Hit
CGACATACAGAGTAGTACAGACAGGAGCCCATATCGAACCCTAATGAGCT80.10134279199391943No Hit
CGACATACAGAGTAGTACAGACAGGAGCATTCCTCATAGTGATAATTAGG80.10134279199391943No Hit
CGACATACAGAGTAGTACAGACGGGGGGAGAAGCTTTCAGTCTCGTACCA80.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT201.6377318E-595.000012
ACATATA201.6377318E-595.000013
ATAGAGT201.6377318E-595.000017
TAGAGTA201.6377318E-595.000018
CATATAG201.6377318E-595.000014
TATAGAG201.6377318E-595.000016
CGATATA156.373673E-495.01
TATACAG156.373673E-495.04
GATATAC156.373673E-495.02
AGAGTAG7950.089.622649
ACAGAGT7800.089.5192267
CAGAGTA7750.089.483878
TACAGAG8000.089.06256
ATACAGA7800.088.9102555
CATACAG7700.088.831174
CGACATA7650.088.790851
GACATAC7550.088.708612
ACATACA7600.088.1253
ATATAGA254.9557315E-576.05
AGAGGAG200.001997948671.259