FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC295CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC295CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACGATACACGCGTATGTAGGGGGGGCAAGAATACTGACATCAGAGT160.7766990291262136No Hit
CGATACGATACACGCGTATGTAGGGGGGGGCAAGAATACTGACATCAGAG140.6796116504854369No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAGAATACTGACATCAGAGT130.6310679611650485No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAACAAAATAACATTCGAAG90.4368932038834952No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAGTTAAAAAGAAGGGCTAT80.3883495145631068No Hit
CGATACGATACACGCGTATGTAGGGGGGCAAGAATACTGACATCAGAGTC60.2912621359223301No Hit
CGATACGATACACGCGTATGTAGGGGGGCCAAATATATTCAATATGGAGA60.2912621359223301No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAAACAAAAGCAACAAAAAT60.2912621359223301No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAGGAGGTGTCACTAAGCTA50.24271844660194172No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAAAACATAATGGACTCCAA50.24271844660194172No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAAATATATTCAATATGGAGA50.24271844660194172No Hit
CGATACGATACACGCGTATGTACGGCGCCATCAAGGGAGCAATTTTACAA50.24271844660194172No Hit
CGATACGATACACGCGTATGTAGGGGGGGAAGAATACTGACATCAGAGTC50.24271844660194172No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAACAAAAGCAACAAAAATG50.24271844660194172No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAATAGCAATAAGACCCAAA40.1941747572815534No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAATACCACTATATGATGCAA40.1941747572815534No Hit
CGATACGATACACGCGTATGTAGGGGGGGCAATAGTTGGAGAAATTTCAC40.1941747572815534No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAGTTAAAAAGAAGGGCTAT30.14563106796116504No Hit
CGATATGATACACGCGTATGTAGGGGGGGGAAGAATACTGACATCAGAGT30.14563106796116504No Hit
CGATACGATACACGCGTATGTAGAGGGGGGAAGAATACTGACATCAGAGT30.14563106796116504No Hit
CGATACGATACACGCGTATGTAGGGGGGCAAAAACATAATGGACTCCAAC30.14563106796116504No Hit
CGATACGATACACGCGTATGTAGGGGGGGGAAAATGGACGAAGGACAAGG30.14563106796116504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG1850.095.01
CGATACA1650.095.06
GATACGA1700.095.02
GATACAC1700.095.07
TACACGC1700.095.09
ATACGAT1700.095.03
TACGATA1750.095.04
ACGATAC1700.095.05
ATACACG1700.095.08
TAGACGC155.707635E-495.09
CGTATGT1800.047.50000414-15
GTATGTA1850.047.516-17
GAGAAGA150.009612163547.570-71
ACAAAAA150.009612163547.542-43
ACACGCG1700.047.510-11
TTGCAAC150.009612163547.582-83
AGTAGTT150.009612163547.562-63
AGAAGAA150.009612163547.572-73
TTGTAAA252.3613382E-547.594-95
CACGCGT1700.047.510-11
Produced by FastQC (version 0.11.5)