FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC294CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC294CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTCGCACGCGTACGACGCAGTGGCGGGCAAAAAACATGTGTGGGGTC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAAGGGGGGAAGTTTTATTATTTGGACAG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGGAGGCGCGCAAAAGAATTAGGCGCGACCTC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAATTAGCGGGCGAGGGGCACGCCGACGGC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACAGAGAGAAAGCTAATGTGCTAATTGAT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGAGCGGGCACAGATGGCTGGATCGAGTG10.9009009009009009No Hit
CGAGTCGCACGCGTGCGACGCAAAAAGGGCAAGATAGTCAAATCAGTCGA10.9009009009009009No Hit
CGAGTCGCACGCGGACGACGCACGGAGGGCAAGTTAAAGCGAAGGGCTAA10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCACGCCGACACTATCAAGAGTTGGAGAAAC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAACGGAACCAAGGTCTTATACAATCCAGC10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCACGGGGAAGAATATCAACAGGAACAGCAG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGACCGGCAATCTCCTGGGTAACAGGTT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGGCGCAAGATTGCTCAGTTCATTGATG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAAGTAGAAGGCCCTCTTTTAAAACCGAA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAACAGAGGCAATTGTCGTTCTAAGTGTCT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACCGGCGGCAATGGGGGTTGGATAGGGAG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACAAAGGGCAAGCGGATCATCAGTCAAGA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGGAGCCGAGTCGCGAGCGGGCGACGCG10.9009009009009009No Hit
CGAGTCGCACTCGTACGACGCAGGAAAAGCAAAAGCAGGGTGACAAAAAC10.9009009009009009No Hit
CAAGTCGCACGCGTACGACGCACGGAACTGAGAAGTTGACAATAACTTAT10.9009009009009009No Hit
CGAGTCGCACGCGCACCACGCACGGGAGGCAAGAGAAGCACCCCGGACTC10.9009009009009009No Hit
CGAGTCGCACGCGGACGACGCAGGACGGCAATGGCCCCAAAAAGGCCTCT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGTACCCGAGTCGCACGCGTACGACGCA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGGAGACCGAATGCACTATTGAAGCACCG10.9009009009009009No Hit
CGAGTCGCACGGGTACGACGCAAATGGCGTAAAAGGATTTTCATTCAAAT10.9009009009009009No Hit
CAAGTCGCACGCGTACGACGCACACAGGCAAAGGCTATGGAACAGATGGC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGGGGAGAGAAAGCTAATGAGATAAGTGG10.9009009009009009No Hit
CGAGTCGCACGCGTAGGACGCCCCACCCGGGGGGGGGGGAACCGGGCGCA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGGGAGAGAAAGCTAATGTGCTAATTGG10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCACCGGCCGCAAAGGCCATGGAGGTTGCTA10.9009009009009009No Hit
CGTGTCGCACGCGTACGACGCATGGAGCCGAACGGATGGACTGGGACAGA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGGCGGCCAAATATATCCAATATGGACAG10.9009009009009009No Hit
CGGGTCGCACGCGTACGACGCACGCAGTCGTTTAAAATGAATCCAAACCA10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCAGCGCCAGAAGAATAATGGACATGATTCC10.9009009009009009No Hit
CGAGTCGAACGCGTACGACGCAGCGAGGGGAAATGAAAAGTGGCGAGAGC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCCGGACGGAGCAACACACGGCGGGAAGAGA10.9009009009009009No Hit
GGAGTCGCACGCGTACGACGCAGACGGGCCGAAGGTACCATGTTTCAACC10.9009009009009009No Hit
CGAGTCGCACGCGGACGACGCACGCGCTGGAACGTTGGGGCGGGGCCCCA10.9009009009009009No Hit
CGAGTCGCACGCGCACGACGCACTAAGACCACTGTGGACCATATAGCCAT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGGGAGGCAAAGGCTATGGAACAGATGGC10.9009009009009009No Hit
CGAGTCGCACGGGGACGACGCACCCCCCCGCGATTGCACGACGCCAGATC10.9009009009009009No Hit
CGAGTCGTACGCGTACGACGCACCAGAAGCAAAGGGGCGTACCACGGCCG10.9009009009009009No Hit
CGAGTTGCACGCGTACGACGCAGAACGCGAAATCAGCAAAAGCAGGTAGA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGGCGCCGAAAACCCCTCAAGTTGTGGA10.9009009009009009No Hit
CAAGTCGCACGCGTACGACGCAGCAGGAGGAAGGCAAGAGAAGAACCCCG10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGAACACAGAAACTGGTATTCGAGAAATT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAATGGCCCGAGCCGCACGCGTGACCAGTA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGGGGCCGGAAAGTGTCTTTGCAGGAAAG10.9009009009009009No Hit
CGAGTCGCACGCGTACGAAGCAAGCATCAAGGAACTGGGCACTCGTGCAA10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCACGCCCATTCGCTTCTGGTAGGCCTGCAA10.9009009009009009No Hit
CAAGTCGCACGCGTACGACGCAGCGCACCAAACGATCATATGAACAAATG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGAGAGCGAATCATGGCCTGGAAAGGGG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGCAAGGGGAACAGTGACCACAGAAGCTG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAATCCGCTAGCCAGCACCATTCTGTTTTC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGTGCGATCGGGAGCGCACACGTGTGAACTCC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGTAGAGGAAACAAGGTACTTTTTTGGA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGGGCCCGAACAACATGGATAGGGCAGT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGGAGGGGAAGGAAAAAGCCACAAAACC10.9009009009009009No Hit
CTAGTCGCACGCGTACGACGCACACCCATTCGCTTTTGGTCTAGTGTGTG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAACCCGCACGCGTGATCAGTAGAAACAAG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGAGCGCGAAGTAGGTACAGATGCAATT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGGAAGCGAATGGGAGGGCAGATGCAGC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGGGGCAAAAACATAATGGACTCCAACAC10.9009009009009009No Hit
CGAGTCGCACGCGTACGGAGCACAACGGGGAAGGGAAGACCCCTCGAGGG10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCAGCGGGGTGAATCAATAATTGTAGAATCT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACCGCGCGAGTCGCACGCGTACGACGCAC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGAGGAGCAAATGGAGTAAAAGGATTTTC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGCAGGGGAAGTGAGAAATGATGATGTT10.9009009009009009No Hit
CAAGTCGCACGCGTACGACGCCGGGGGGGGAACAGTGACCACAGAAGCTG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACGAAGGGGGAAAATACCGGGTTGTTGAA10.9009009009009009No Hit
CGAGTTGCACGCGTACGACGCAATGGGGACAACCTGGAACCTGGAACCTT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAACGGGGGCAGTAGTTGTAAGGCTTGCAT10.9009009009009009No Hit
CGAGTGGCACGCGTACGACGCAAGGCCCCTTGCCCAATTAACACATATTC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACCCAGGCAGTTGGGCTCGACTATCGTCGAAGG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAACTCGCGAGATACTCACTAAGACCTCTC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAATAAGACTGTTGAATACAGATTTTGCCA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGGAGAGGAAATGCCCGCTAATTTCACG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGGCACCCCCCCGCGTGATCAGTAGAAA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACTGGGAAATCTAGTGGTACCGAGATATG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGGGCCGGCAACGGTGTTTGGATAGGGAG10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCAAGGCAGGTGACAAAAACATAATGGACTC10.9009009009009009No Hit
CAAGTCGCACGCGTACGACGCAGCACCCGGAAGCGAATGGGAGTGCAGAT10.9009009009009009No Hit
CGAGTCGCACGCGGACGACGCACCGGGCAAAAGCAGGTAGATATTTAAAG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAACGGGGGGAAACATCTTCTCCTATTTCA10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCACTTGGGGCAACATCTTCTCCTATTTCAT10.9009009009009009No Hit
CGAGTCGCACGCGAACGACGGACGGAAGGCTAGCGAAAAAAGCAGCACTC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCATCGTACCTACTTCGCGCTACCTTTCTGA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACCCAACAGAAACAAGGCATTTTTTCATGAAGG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCGGCCGTTAGGGTGGCATTCACCGGCGGCA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACCCCCCCAAATAGGCCTCTAGATCGAAT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCACCGACAGAAGCTGCTTTTGGTCTAGTGC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGACGCCCACGGTCGATTCGAGCCATGCC10.9009009009009009No Hit
CGAGTCGCACGCATACGACGCCACAGGCCAAATCTATTCAATCTGGACAG10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCACCTGGAGAAACTGTGATGAGAATGGGAG10.9009009009009009No Hit
CGGGTCGCACGCGTACGACGCACACCCAGCAATGTTACATTTACCCAAAT10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGAAGCGCGAAGTAGGTACAGATGCAATT10.9009009009009009No Hit
CGAGGCGCACGCGGACGACGCACGGGCGCGAAGAAGAGACAGATGTAATT10.9009009009009009No Hit
CGTGTCGCACGCGTACGACGCAATGACGGCAACCTCCATGGCCTCTGCTG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAAGACGGCCATGGTGTTTGGGTAGGGAG10.9009009009009009No Hit
CGAGTTGCACGCGTACGACGCAACGGGCCCAATCATGACACGAACAAAGG10.9009009009009009No Hit
CGGGTCGCACGCGTACGACGCAGTGACAGGAAAGTGTCTTTGCAGGAAAG10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCACCGCAATGTTACATTTACCCAAATGCAC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGGAGAACACAATCTGGACTAGCGGGAG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAAGGGGGGAATCAAGGAATTCTTTGAAAA10.9009009009009009No Hit
CGGGTCGCACGCGTACGACGCACGGACAGAAATTTGAGGAAATAAGGTGG10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCATGGGGGCAACTGGAGCAAAAGGATTTTC10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAACGGAGAGCAAGCTAATGTGCTAATTGG10.9009009009009009No Hit
CAAGTCGCACGCGTACGACGCACCCGAGCGAAATTTCTGGAGGGGTGAAA10.9009009009009009No Hit
CGAGTCGCACGCGTACGACGCAGGCCATCTAATTTTTTAGAAGGATCTAT10.9009009009009009No Hit
TGAGTCGCACGCGTACGACGCACCAGAACAGAGTAGGATACAATTCGACA10.9009009009009009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC100.094.999994
CACGCGT100.094.999998
GCACGCG100.094.999997
AGTCGCA100.094.999993
TCGCACG100.094.999995
CGCACGC100.094.999996
ACGCGTA100.094.999999