FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC292CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC292CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5896
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGAGTCTATATACGTCGGGGGGGGGAAAATGGACGAAGGACAAGG210.35617367706919945No Hit
CGAGATGAGTCTATATACGTCGAGGCCAAGGGTGTTGCCTCTTCCTTTTA210.35617367706919945No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAAATGGAGTAAAAGGATTTT120.20352781546811397No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAATAGTTGGAGAAATTTCAC120.20352781546811397No Hit
CGAGATGAGTCTATATACGTCGGGGGGGACAAGATTGGTCTTGTCTTTAG100.16960651289009498No Hit
CGAGATGAGTCTATATACGTCGGAGGGGGGAAAATGGACGAAGGACAAGG100.16960651289009498No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGAAATACCAATCAGCAAAAGG90.15264586160108548No Hit
CGAGATGAGTCTATATACGTCGGGGGGGCAAATGGAGTAAAAGGATTTTC90.15264586160108548No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGAACAGTGACCACAGAAGCTG90.15264586160108548No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGCAATAGTTGGAGAAATTTCA90.15264586160108548No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGAAAATGGACGAAGGACAAGGG80.13568521031207598No Hit
CGAGATGAGTCTATATACGTCGCGGCCAAGGGTGTTGCCTCTTCCTTTTA80.13568521031207598No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGCAACATCTTCTCCTATTTCAT60.10176390773405698No Hit
CGAGATGAGTCTATATACGTCGAGGAGCTAAGAGAGCAATTGAGCTCAGT60.10176390773405698No Hit
CGAGATGAGTCTATATACGTCGAGGGGGGGAAAATGGACGAAGGACAAGG60.10176390773405698No Hit
CGAGATGAGTCTATATACGTCGGGGGGGGGAATACCACTATATGATGCAA60.10176390773405698No Hit
CGAGATGAGTCTATATACGTCGGGGGGAGCAATAGTTGGAGAAATTTCAC60.10176390773405698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA5300.095.000017
CGAGATG4950.095.000011
TGATGAG254.068279E-795.03
GTGATGA254.068279E-795.02
GTCTATA5350.095.09
CGTGATG254.068279E-795.01
AGTCTAT5350.095.08
TGAGACT156.2908616E-494.999996
AGACTAT156.2908616E-494.999998
GAGACTA156.2908616E-494.999997
GACTATA156.2908616E-494.999999
GAGATGA4950.094.040412
TGAGTCT5500.091.5454566
GATGAGT5500.090.6818164
ATGAGTC5400.090.601855
AGATGAG5550.088.153153
CAAGATG254.848863E-576.01
GATGAGA200.001972132671.254
ATGAGAC200.001972132671.255
CGTCGAG451.8735591E-1047.50000418-19
Produced by FastQC (version 0.11.5)