FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC263CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC263CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGAGCTTGAAAAGGAG60.32017075773745995No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAGTAGAAAAGAAAAGGGAG40.21344717182497333No Hit
CGTCGTACGATAGCACTAGTATCGGCTCTAGATTAAAGATTTAGATGCAG40.21344717182497333No Hit
CGTCGTACGATAGCACTAGTATACGCGCCCATGATCCAAACACCTCCCAC30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGTGGCACGATGAAACC30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGCTAGGACAAGTGAT30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAATACCAATCAGCAAAAGG30.16008537886872998No Hit
CGTCGTACGATAGCACTAGTATCGGCCCCATGATCCAAACACCTCCCACC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCCGCCCCCATGATCCAAACACCTCCCAC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGGAGTAGAAAAGAAAAGGGGG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGCCACAACTACTTCAGTGCATGTGTGA20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGAGGGAAAAGGAGGAGGAAGGAGAA20.10672358591248667No Hit
CGACGTACGATAGCACTAGTATCGGCAAAAGCAGGGAGCTTGAAAAGGAG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGAGGGGGAAGTAGAAAAGAAAAGGGAG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGGGGGGGAAAACAAAAGCAACAAAAAT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGGCAAAAGCAGGGTTTATTTATTTAGG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCCGCCCCTGGAAGGACAAATCTTCTGGC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGCGGGGGCAAATAGGTTGACTAAGTCTT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAACCTGGAGTGGGAGGGATT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATCGCCGGGGAAGAAGTGGACGTTTGAGTG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGCCGGGGAAGAAGTGGACGTTTGAGTG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGCGGATTCCTCACAGCCTGGTGCT20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGCAAGAAGGGGATTGAACTCAGC20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAGGAGAAAAGAAAAGGGAG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGCAAAAACATAATGGACTCCAACA20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGCAAATTGACTTTCTCTGGTTG20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGCAAAAACATAATGGACTCCAA20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGAAAAGGAGGAGGAAGTAGAA20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGAGGGGGGAAAAGGAGGAGGAAGTAGAA20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGACGAGAGCGAGACTTCATCTCCA20.10672358591248667No Hit
CGTCGTACGATAGCACTAGTATGGGGGGGGCAAATAGGTTGACTAAGTCT20.10672358591248667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGC155.6138297E-495.08
CGATAGC1500.095.08
GTCGTAC1550.095.02
GTACTAT155.6138297E-495.05
CGTACTA155.6138297E-495.04
TACGATA1500.095.06
CGTCGTA1550.095.01
TCGTACT155.6138297E-495.03
TCGTACG1500.095.03
ACGATAG1500.095.07
TATAGCA155.6138297E-495.09
TACTATA155.6138297E-495.06
GATAGCA1450.095.09
ACTATAG155.6138297E-495.07
GTACGAT1650.095.05
CGTACGA1600.094.999994
GTATGGG600.047.518-19
GTATGGC150.00953661147.518-19
GTATGCG150.00953661147.518-19
TATGCGG150.00953661147.520-21
Produced by FastQC (version 0.11.5)