FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC258CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC258CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26029
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGAGTATCTGAGTATACGCGGGGGGGGAAAGGAAATGTAATGCATTT660.253563333205271No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGAGCAAGAGAG590.22667025241077263No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGTGAAAGATGT560.21514464635598754No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGGAATTGATAGGCTCATGAAAA520.19977717161627417No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGGAAGCTTCA480.18440969687656075No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGCAAAAGCAGGAGCAAGAGAGA430.16520035345191902No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGAAAACAGATTTTAAGTTGTAAG400.15367474739713397No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGGAAGCTTTCAGTCTCGTACCA380.14599101002727727No Hit
CGTAGAGTATCTGAGTATACGCGGGGGGGCAAGTGTGAGTGAATGTGTGT350.1344654039724922No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGAGTTGAACT270.10373045449306542No Hit
CGTAGAGTATCTGAGTATACGCCGGCGCCAAACCTTATGTGAAATTGTAA270.10373045449306542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAG156.4952736E-495.1811141
GTAGAGC254.4945773E-794.998082
CATCTGA254.4945773E-794.998088
TATCCGA156.545307E-494.998088
TAGAGCA254.4945773E-794.998083
ATCCGAG156.545307E-494.998089
GCATCTG254.4945773E-794.998087
ATCTGAA201.711998E-594.998089
GTAGAGT24600.094.8052
GTATCTG24250.094.80227
ATCTGAG24900.094.616569
TAGAGTA24700.094.6134643
AGTATCT24300.094.607136
TATCTGA24250.094.606338
GAGTATC24550.094.417645
CGTAGAG25550.094.2497941
AGAGTAT24750.094.2304154
GATCTGA650.087.690548
GAGGATC600.087.0815665
AGGATCT600.087.0815666
Produced by FastQC (version 0.11.5)