FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC257CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC257CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATAGTCGTACGTATACATCGGGGGGGGAACAGTGACCACAGAAGCTG110.26290630975143403No Hit
CGCATAGTCGTACGTATACATCCGGCCATCTGTTCCATAGCCTTTGCCGT110.26290630975143403No Hit
CGCATAGTCGTACGTATACATCGGGGGGGCAAGAGAAGAACCCCGCACTC70.16730401529636713No Hit
CGCATAGTCGTACGTATACATCGGGGGGCAAGAGAAGAACCCCGCACTCA60.14340344168260039No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAAGAGAAGAACCCCGCACTC60.14340344168260039No Hit
CGCATAGTCGTACGTATACATCGGGGGGGGAAGGGGTCGCCTGGAAGTCA60.14340344168260039No Hit
CGCATAGTCGTACGTATACATCGGGGAGGCAAGAGAAGAACCCCGCACTC50.11950286806883366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT156.1590626E-495.07
GTCGTAC3400.095.07
CGTAGTC156.1590626E-495.03
ACGTACG156.1590626E-495.08
GTATAGT253.8557846E-795.02
ATAGTCG3400.095.04
AGACGTA156.1590626E-495.06
GACGTAC156.1590626E-495.07
AGTCGTA3500.095.06
CGTACGT3600.095.09
ATAGACG156.1590626E-495.04
CATAGTC3250.095.03
TCGTATG156.1590626E-495.08
GCGTAGT156.1590626E-495.02
CGCATAG3450.095.01
TATAGTC253.8557846E-795.03
TAGACGT156.1590626E-495.05
CGCGTAG156.1590626E-495.01
GTAGTCG156.1590626E-495.04
GCATAGT3250.095.02
Produced by FastQC (version 0.11.5)