FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC248CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC248CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10470
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACAGCTACTACTACTCTAGGGGGGGGAAGCTTCGTGCTGGAGAAAA430.4106972301814709No Hit
CGATACAGCTACTACTACTCTAATTCGCTTCTGGTAGGCCTGCAAATTTT310.29608404966571156No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTCGTTT300.28653295128939826No Hit
CGATACAGCTACTACTACTCTACGGAGCAGAAATGAAAAGTGGCGAGAGC280.26743075453677173No Hit
CGATACAGCTACTACTACTCTAACGCGAAGTAGGTACAGATGCAATTGTC250.2387774594078319No Hit
CGATACAGCTACTACTACTCTAAGACCACTGTGGACCATATGGCCATAAT220.21012416427889208No Hit
CGATACAGCTACTACTACTCTAAGGCCACTGTGGACCATATGGCCATAAT210.20057306590257878No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGGTGTTT170.1623686723973257No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCGATCT170.1623686723973257No Hit
CGATACAGCTACTACTACTCTACCCCGCACTCAGAATGAAGTGGATGATG170.1623686723973257No Hit
CGATACAGCTACTACTACTCTAACGCCATGTCAAGCTTTCAGGTAGACTG160.15281757402101243No Hit
CGATACAGCTACTACTACTCTAACACCATGTCAAGCTTTCAGGTAGACTG150.14326647564469913No Hit
CGATACAGCTACTACTACTCTACTGAGCAGAAATGAAAAGTGGCGAGAGC150.14326647564469913No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTAGTTT150.14326647564469913No Hit
CGATACAGCTACTACTACTCTACAGAGCAGAAATGAAAAGTGGCGAGAGC140.13371537726838587No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGGTGACAA140.13371537726838587No Hit
CGATACAGCTACTACTACTCTACGGCCCCTTGCCCAATTAGCACATTAGC140.13371537726838587No Hit
CGATACAGCTACTACTACTCTACTAACCATTAATCTTGCGTAGCAGAGGC130.12416427889207259No Hit
CGATACAGCTACTACTACTCTAAGCCGAGATCGCGCAGAGACTGGAAAGT130.12416427889207259No Hit
CGATACAGCTACTACTACTCTAGGGGGGGGAAAGGAAATGTAATGCATTT130.12416427889207259No Hit
CGATACAGCTACTACTACTCTAGGGGGGCTATATGAAGCAATCGAGGAGT120.1146131805157593No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGCCAAATA120.1146131805157593No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGCCGTTT120.1146131805157593No Hit
CGATACAGCTACTACTACTCTACGGCAAAGGCTATGGAACAGATGGCTGG120.1146131805157593No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGCAAAAGCAGGTCAAATA110.10506208213944604No Hit
CGATACAGCTACTACTACTCTACGTCCCCTTGCCCAATTAGCACATTAGC110.10506208213944604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACT10000.094.520237
GCTACTA9950.094.5178458
CAGCTAC9900.094.515446
TACAGCT9900.094.515444
ACAGCTA9850.094.513015
CGATACA10000.094.4974061
ATACAGC10000.094.045263
GATACAG10100.093.58442
CTCTAGG3450.047.49761218-19
CTCTACC550.047.49761218-19
CTCTAAC550.047.49761218-19
GAGCGTT252.7329472E-547.4976194-95
TTGCATG357.434937E-847.4976194-95
ACTGTAA301.4231427E-647.4976182-83
ATCTTCC252.7329472E-547.4976146-47
TCTAGGC750.047.4976118-19
TCTAGGA205.2682497E-447.4976118-19
ATCCAGG205.2682497E-447.4976176-77
AGGACTG252.7329472E-547.4976180-81
ACCATCC205.2682497E-447.4976172-73
Produced by FastQC (version 0.11.5)