FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC216CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC216CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20892
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTGTGATAGTATCTCGTAGAAACAAGGTAGTTTTTTACTCTAGCTCT670.32069691748037527No Hit
CGAGTGTGATAGTATCTCGTAGGGGGCGGGAACAACTAGGTCAGAAGTTT550.26325866360329314No Hit
CGAGTGTGATAGTATCTCGTAGCCGCAAGTATAATTGGGATTGTGCACTT530.2536856212904461No Hit
CGAGTGTGATAGTATCTCGTAGATCAGCAAAAGCAGGTAGATATTGAAAG490.23453953666475205No Hit
CGAGTGTGATAGTATCTCGTAGCGGCAACGATTCAAGTGATCCTCTTGTT490.23453953666475205No Hit
CGAGTGTGATAGTATCTCGTAGGGGGGGCAAATGCAACGATTCAAGTGAT420.2010338885697875No Hit
CGAGTGTGATAGTATCTCGTAGCCTATACATATGCAGACACAATATGTAT360.17231476163124643No Hit
CGAGTGTGATAGTATCTCGTAGAGAGGATTCTTCTTTCAGAATCCTCTCT350.1675282404748229No Hit
CGAGTGTGATAGTATCTCGTAGGAAGGGGCAAAAGTAACAGACTTAGAAA320.15316867700555237No Hit
CGAGTGTGATAGTATCTCGTAGAAAGGGGTTTGAGATGATTTGGGATCCT320.15316867700555237No Hit
CGAGTGTGATAGTATCTCGTAGTTTTTTACTCTAGCTCTATGCTGACAAA320.15316867700555237No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGCAGTTTTTTACTCTAGCTCT310.14838215584912887No Hit
CGAGTGTGATAGTATCTCGTAGAAGGCGGGAACAACTAGGTCAGAAGTTT300.14359563469270534No Hit
CGAGTGTGATAGTATCTCGTAGGAGGCGGGAACAACTAGGTCAGAAGTTT280.1340225923798583No Hit
CGAGTGTGATAGTATCTCGTAGCCACAAAAAGAAATGCTGCTCCCACTAG270.1292360712234348No Hit
CGAGTGTGATAGTATCTCGTAGAAGCAAGGTAGTTTTTTACTCTAGCTCT270.1292360712234348No Hit
CGAGTGTGATAGTATCTCGTAGTTGCCGCAAGTATAATTGGGATTGTGCA260.1244495500670113No Hit
CGAGTGTGATAGTATCTCGTAGATCAGCAAAAGCAGGCAGATATTGAAAG260.1244495500670113No Hit
CGAGTGTGATAGTATCTCGTAGAAACGAGGTAGTTTTTTACTCTAGCTCT240.11487650775416428No Hit
CGAGTGTGATAGTATCTCGTAGGAAGGGCCAAAAGTAACAGACTTAGAAA230.11008998659774076No Hit
CGAGTGTGATAGTATCTCGTAGAAACAAGGTCGTTTTTAAATTATTCGAA230.11008998659774076No Hit
CGAGTGTGATAGTATCTCGTAGGAAGGACCAAAAGTAACAGACTTAGAAA220.10530346544131725No Hit
CGAGTGTGATAGTATCTCGTAGACAAACTGGAAGTCAAAACAACACTGGA220.10530346544131725No Hit
CGAGTGTGATAGTATCTCGTAGCAGGCGGGAACAACTAGGTCAGAAGTTT210.10051694428489374No Hit
CGAGTGTGATAGTATCTCGTAGTCTGTTGTAGTTGAATACTTGTGAGGGG210.10051694428489374No Hit
CGAGTGTGATAGTATCTCGTAGAAGGCCCTCTTTTTAAACCGTGTTTAAA210.10051694428489374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGT156.526371E-495.07
GGTGATA450.095.05
TGGTAGT156.526371E-495.07
GAGTGCG156.526371E-495.02
TGATCGT156.526371E-495.07
TGATAGA156.526371E-495.07
CGAGGGT450.095.01
AGTGCGA156.526371E-495.03
AGTGTGG156.526371E-495.03
GGTAGTA156.526371E-495.08
ATAGAAT156.526371E-495.09
CGAGTGC156.526371E-495.01
TGTGGTA156.526371E-495.05
GTGGTAG156.526371E-495.06
GTGCGAT156.526371E-495.04
AAAGTAT156.526371E-495.09
GATGGTA156.526371E-495.08
GTGATAG19500.094.512826
CGAGTGT19350.094.509041
TGATAGT19200.094.505217
Produced by FastQC (version 0.11.5)