FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC193CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC193CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259955
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGTATAGACGTGATACTGTGTCCATGGACACAGTAAACAGAACACA35071.3490796484006848No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGAACAGGAACAGGATACACC28041.0786482275778502No Hit
CGTATGTATAGACGTGATACTGAAGAGCTTCCCAGCACTGGGATGCTCTT21440.824758131215018No Hit
CGTATGTATAGACGTGATACTGGCGGGGAGCGCCAGGATGCCACAGAAAT19050.7328191417745379No Hit
CGTATGTATAGACGTGATACTGAAACGAGAAAGCTCTTATCTCTTGTTCT17730.6820411225019715No Hit
CGTATGTATAGACGTGATACTGGACCACAACTGCCTGTCTTATCATTAGG16400.630878421265219No Hit
CGTATGTATAGACGTGATACTGAAAAGGGAAAGATAGTCAAATCAGTCGA10580.40699351810890344No Hit
CGTATGTATAGACGTGATACTGAAGATCTTGTATGAGGCCTGTCCATCAC10060.38699005597122577No Hit
CGTATGTATAGACGTGATACTGAGGCCTATTCAACTGGTGCACTTGCCAG9720.37391086918889804No Hit
CGTATGTATAGACGTGATACTGTTTTCTTAGGGTCCTTCCCAGCACTGGG8120.31236175491912066No Hit
CGTATGTATAGACGTGATACTGGGGGGGGGAAAATGGACGAAGGACAAGG8050.30966898116981784No Hit
CGTATGTATAGACGTGATACTGGTGGGGAGCGCCAGGATGCCACAGAAAT8010.3081302533130734No Hit
CGTATGTATAGACGTGATACTGGGGGGGAGCGCCAGGATGCCACAGAAAT7920.3046681156353984No Hit
CGTATGTATAGACGTGATACTGGAGGGGGGAAAATGGACGAAGGACAAGG6840.26312246350329865No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGCATTTTTTCATGAAGGAC6460.24850454886422652No Hit
CGTATGTATAGACGTGATACTGTTACATTCTTTTCTAGTACTGTGTCTAC6330.2435036833298071No Hit
CGTATGTATAGACGTGATACTGAAACAAGGCATTTTTTCATGAAGGACAA6310.24273431940143486No Hit
CGTATGTATAGACGTGATACTGCAAGCCCATACACACAAGCAGGCAGGCA6080.23388663422515438No Hit
CGTATGTATAGACGTGATACTGAAGCGAATGGGAGTGCAGATGCAGCGAT5960.22927045065492105No Hit
CGTATGTATAGACGTGATACTGAGGAGCTAAGAGAGCAATTGAGCTCAGT5650.21734530976515168No Hit
CGTATGTATAGACGTGATACTGATGCAATTGTCGTTCTAAGTGTCTCGCT5370.20657421476794063No Hit
CGTATGTATAGACGTGATACTGGCGGGGGGCGCCAGGATGCCACAGAAAT5250.2019580311977073No Hit
CGTATGTATAGACGTGATACTGATTCCCAAGTTGAATTGAGTGGCTAATC5180.1992652574484045No Hit
CGTATGTATAGACGTGATACTGGACGGATCATGATCTCAGAGAACTCTTC4740.18233925102421575No Hit
CGTATGTATAGACGTGATACTGAAAAGCAGAAAGCACCATCCTCTCTATT4730.18195456906002963No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAGAGGATGGTGC4610.1773383854897963No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAAAGCACCATCC4470.1719528379911908No Hit
CGTATGTATAGACGTGATACTGGGAGGCTGGTGTTTATAGCACCCTTGGG4450.17118347406281856No Hit
CGTATGTATAGACGTGATACTGAACAAAACTCCCGCTATATCCTGACCAC4260.1638745167432825No Hit
CGTATGTATAGACGTGATACTGTTGTGGTCCAGTATCGTCTAATGGAGCA4130.15887365120886307No Hit
CGTATGTATAGACGTGATACTGAAGAGCACACAGAATGCCATTGACGAGA3980.15310342174607142No Hit
CGTATGTATAGACGTGATACTGAAAAGGGGAAGATAGTCAAATCAGTCGA3930.15118001192514088No Hit
CGTATGTATAGACGTGATACTGGGGGGGGAAAATGGACGAAGGACAAGGG3890.14964128406839647No Hit
CGTATGTATAGACGTGATACTGCAGAGCAGAAATGAAAAGTGGCGAGAGC3870.14887192014002423No Hit
CGTATGTATAGACGTGATACTGGAAAAGGGAAAGATAGTCAAATCAGTCG3870.14887192014002423No Hit
CGTATGTATAGACGTGATACTGCAGAGACTGGAAAGTGTCTTTGCAGGAA3840.1477178742474659No Hit
CGTATGTATAGACGTGATACTGCGCAGAGACTGGAAAGTGTCTTTGCAGG3660.14079359889211596No Hit
CGTATGTATAGACGTGATACTGCAGCGATTCAAGTGATCCTCTCGTCATT3570.13733146121444095No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGGTAGATAATCACTCAATGA3570.13733146121444095No Hit
CGTATGTATAGACGTGATACTGATCCCCTCAGAATGAGTGCTGACCGTGC3550.13656209728606875No Hit
CGTATGTATAGACGTGATACTGTGATGAGAATGGGAGACCTTCACTACCT3550.13656209728606875No Hit
CGTATGTATAGACGTGATACTGTTTGCTGTGTCCATGGAACAGGAACAGG3520.13540805139351042No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGTCAAATATATTCAATATGG3500.13463868746513818No Hit
CGTATGTATAGACGTGATACTGATCCAAAATGGAAGACTTTGTGCGACAA3490.1342540055009521No Hit
CGTATGTATAGACGTGATACTGCAAAGCAGGTAGATATTTAAAGATGAGT3490.1342540055009521No Hit
CGTATGTATAGACGTGATACTGAAATGGGAGGCTGGTGTTTATAGCACCC3470.13348464157257986No Hit
CGTATGTATAGACGTGATACTGTTTACACCACAAAAGGATATGCTGCTCC3450.13271527764420765No Hit
CGTATGTATAGACGTGATACTGAGCAGAAATGAAAAGTGGCGAGAGCAAT3440.13233059568002153No Hit
CGTATGTATAGACGTGATACTGAAGAAACAAGGCATTTTTTCATGAAGGA3380.13002250389490488No Hit
CGTATGTATAGACGTGATACTGTGGTCCAGTATCGTCTAATGGAGCAAAT3290.1265603662172299No Hit
CGTATGTATAGACGTGATACTGAACACACCAATACTCAGAAAAGAACAGA3280.1261756842530438No Hit
CGTATGTATAGACGTGATACTGATACTCTTAAATCTTCAATTGGTGTGGA3150.12117481871862439No Hit
CGTATGTATAGACGTGATACTGGGGGGAGCGCCAGGATGCCACAGAAATC3050.11732799907676328No Hit
CGTATGTATAGACGTGATACTGAGGCTCTCATGGAATGGCTAAAGACAAG3010.11578927122001886No Hit
CGTATGTATAGACGTGATACTGAAGGACAAGTTAAATTCATTATTTTTGC2890.11117308764978555No Hit
CGTATGTATAGACGTGATACTGATTGCCCCCAGGGAGACTACCAGTACCA2810.10809563193629668No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGTATTTTTTCATGAAGGAC2800.10771094997211056No Hit
CGTATGTATAGACGTGATACTGATCTTCAGAATAGAAAAGGGAAAGATAG2790.10732626800792444No Hit
CGTATGTATAGACGTGATACTGAAACAAGGTATTTTTTCATGAAGGACAA2780.10694158604373835No Hit
CGTATGTATAGACGTGATACTGGTTGAAAGACACCCACGGTCGATTCGAG2730.1050181762228078No Hit
CGTATGTATAGACGTGATACTGGTGGGTAGCGCCAGGATGCCACAGAAAT2710.10424881229443557No Hit
CGTATGTATAGACGTGATACTGATGCCAGACTCGAAGTCGACCCTGGCAT2690.10347944836606336No Hit
CGTATGTATAGACGTGATACTGGGGGGCTGGTGTTTATAGCACCCTTGGG2670.10271008443769114No Hit
CGTATGTATAGACGTGATACTGATGGACGACTAAAGCAGAAAGCACCATC2640.10155603854513282No Hit
CGTATGTATAGACGTGATACTGAGGAGATTTCATCGATTATGAGGAGCTA2630.1011713565809467No Hit
CGTATGTATAGACGTGATACTGACAGGCAGTTGTGGTCCAGTATCGTCTA2620.10078667461676058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG450.095.176531
CGTATAT450.095.176531
TAGACCT156.613925E-494.993479
TGGACGT450.094.993469
TATAGAT450.094.993467
TGTTTAG500.094.9934545
TTTAGAC500.094.9934547
CTAGACG500.094.9934548
CGTATAG201.7418768E-594.9934545
GTTTAGA500.094.9934546
CTATAGA254.6124296E-794.9934546
TAAACGT201.7418768E-594.9934549
ACGTATA201.7418768E-594.9934544
TAGACGT253850.094.9186259
TATAGAC253450.094.899767
GTATAGA253700.094.881136
ATAGACG254350.094.769378
CGTATGT255500.094.710891
TGTATAG254550.094.6762545
GTATGTA254750.094.564642
Produced by FastQC (version 0.11.5)