FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC179CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC179CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGACGTACAGCGCGTGTAGGATTCGCTGAGCTCAGTGTCATCATTT10.7299270072992701No Hit
CGAGTGACGTACAGCGCGTGTACCATCTGCCTAGTCTGATTAGCAACCTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGACCAAAAAAGGAAGACGGAAAATAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGCCCTCCCGCCAGGATCGTATTGGACGA10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAGGCAGCAAAAGCAGGTCAAATATATTCA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGGGAAGAAAAAACAAAGGATACGAAAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGACAGAAATTTCTGTCCCAATTGCT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGAGTAGCGGGGCCTAGGGGGCTGCCTGTTCGTT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATCAGCAAAAGCAGGTCAAATATATTCAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGCCGGGCGCGGGGGGGACAGCGCGGGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAAAACGACAATTGCATCTGTACCTACTT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGACCGGAAAGGGTCTTTGCAGGAAAG10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAAGGGAGAGAAAGCTAAGTATGCTAGAGT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGAGGCGGAAAAAGAAGACTACATATCC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGCGGGAGTTTAGTTCAACCCAGAAGCAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAACGGCAAAATAACAGACACTATCAAGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGAAGGGAAAGATAGTCCTTTCGACTGC10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGCGGGGGTTTAGTTCAACCCAGAAGCAA10.7299270072992701No Hit
CGGGTGACGTACAGCGCGTGTAAAAGGGACAAAAACATAATGGACTCCAA10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTACGCGTGATCAGTAGAAACAAGGGTGTTT10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGAGAGAAGAAATAAATACCTAGAAGAGC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGCTGGGCGCGGGACGGAAAGCGCGCGGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACCATCTGCCTAGTCTGATTAGCAACCTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACACACACCAATACTCAGAAAGGGGAAAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGGGAAAGAAGTCCTCGGGCTATGGGGC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAATAACGGACACTATCAAGAGTTGGAGAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGGGAACAAAAGGATATGCTGCTCCCGC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGACGGAAAGTGGATGAGAGAACTCAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGACAGACAAAACTCCCGCTATATCCTG10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGGGAGGGCAATTGGGACAGAAATTTGAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGCAAGAATACTGACATCAGAGTCAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAACAAAGGCAATACTAGTAGTTCTGCTAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTTTATCAGCAAAAGCAGGAGTTTAAAATGAAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGAAAGGGAAAGACACCCACGGACGATT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACAGAGGGCAACCCACAGTGTCACTGTTT10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGAAAGAGGAATCCAGCGAGACACTTAGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGCAGCAACTGCATTGGCCAACACCA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATTCATCTTTTCAATAACAGAATTTACTT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACAGACCCCAACTGCATTTTTGACATCCT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGGAAAATGAGGTCTTCAATATCAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATTTCTGTGGAAACCATGGCGTCTCAAGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACGACGGCAAAATAACAGACACTATCAAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAAAGGGGAAAGATAGTCGAATCAGGATA10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAGGGATAACAGGAGCATTCCTCATAGTGA10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAAGGGGGGCAAGAATACTGACATCAGAGG10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAGCAGAATGGGGCTGCCTGTTAAGTCGA10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAAACGGCAAAATAACAGACACTATCAAGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGATAACAGGAGCATTCCTCATAGTGAT10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAGCGGGCGTTTAAAATGAATCCAAACCAA10.7299270072992701No Hit
CATGTGACGTACAGCGCGTGTAGTCGAGCTTGCGGAAAAGACAATGAAAG10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAGCGGGAGCAATTGGGACAGAAATTTGAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAACCCGACGCGCGATCAGCAGAAACGAGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAAGCACAAAAATGAAACCGGCAATGGCC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGCCTGCAACTTTTCAAGAAGGTCATCT10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAAGTGTCTCGCTGGATTCCTCTTTCAAGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACGAGCAGCAAAAGCAGGTCAAATATATT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATCCAAACAAAAAGATAATAAGCATTGGT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACGCGGCAGAAGCAGGAGTTTAAAATGAA10.7299270072992701No Hit
CGGGTGACGTACAGCGCGTGTATGCAAAAGCAGGGTGACAAAAACATAAT10.7299270072992701No Hit
CGGGTGACGTACAGCGCGTGTACATTCTCAATGGAAAGAAATGCTGGATC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGAAGGCCGGGGAACAGGCAGTGTTTATATTG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGCGAACAGAACCTTGTCCCTGGATT10.7299270072992701No Hit
CGAGTGACGTACAGCGCGTGTACCGCAAAGAACAATAGGGAGGAAAAAAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGGGGGGAAAGGGAGTTCGGGGGTGGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGGCGGGGAAAACAAAAGCAACAAAAAT10.7299270072992701No Hit
CGCGTGACCTACAGCGCGTGTAACGAAGGCAATACTAGTAGTTCTGCTAT10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAAGAAGTCCTCGTGCTATGGGGCATTCAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACATGTGATTTCACTAGAATCAGGATAAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGGAGAAAAACATAATGGACTCCAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGGGGAGAAACAATATATTGAGAACAC10.7299270072992701No Hit
CGCGAGACGTACAGCGCGTGTAGCAGGGGCAATTGTCGTTCTCAGTGTCA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGGAGGGAAGGCAAATAGATGTGTTCAAGGAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAATGGACTCAATGACCGAAGCGGAAATCC10.7299270072992701No Hit
CGTGTGAGGTACAGCGCGTGTAGAAAGGGAAAGGTACCCTTCCCTTTCAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACTGCTCTATCCAATCTTGTCACCTCTGA10.7299270072992701No Hit
CGGGTGACGTACAGCGCGTGTAAGCCAAGGAAATTGGAAACGGCTGCTTT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAACGGACACATTCAGACTCTTGTGTTCTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGGGGCAAATGGAGTAAAAGGATTTTC10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAGGACGAAGAACAAGGGTTGCTTATGAAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGGGTACGGGGCCAAAAATATTCAATGTGGAGAG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAAACCCAGGGATCATTAATCAGGGACTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGAGGGCAAGAATACTGACATCAGAGT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATGAGGCCCATGCAACTGGCAAGTGCACC10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAAAGTACAACGGCATAATAACATGCACAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTATGACCGATGGACCAAGTGATGGACAGGC10.7299270072992701No Hit
GGCGTGACGTACAGCGCGTGTAAGCAGCAAAAGGGGGGTGACAAAAACAT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGAAAGGGGAAAGACACCCACGGTCGATT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGCAGAAATTTGAGGAAATAAGCTG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGGAGGCAAAATCTGGTAAATCCTTGT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGAGGCAAAAGCAGGAGGTTAAAATGAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAATGGTGAAATTTCTCCAACTATTGCTCC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGGGTAGCGTTGGGGCAACAATATATTGCGAACC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAATTGCCGTTCTAAGTGTCTCGCTGGATT10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTACAGCAAAAGCAGGTAGATATTTAAAGAT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGGGAAGACAAGCATAACGGGAAACTATG10.7299270072992701No Hit
CGGGTGACGTACAGCGCGTGTACCAAAAGCAAGGTGACAAAAACATAATG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAACCGGGAGGAAAAATAACATTCGAAGCA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAAGACGGAAAACAAACCTGTATGGGTT10.7299270072992701No Hit
CGGGTGACGTACAGCGCGTGTAAGTGGCGAGAGCAATTGGGACAGAAATT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGGACAGAAGCGAATGGGAGTGCAGATGCAGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGAAGACGAAAAACAAACCTGTATGGGT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGTAGTGGGAATCAACATGAGCAAAAAG10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAAGGCGGAAATAGAAATAAGACCCAAAG10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGGGCCAAGGAGGTGTCACTAAGCTATTC10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAGGGGGACAAAAACATAATGGACTCCAAC10.7299270072992701No Hit
CGCGTGACGTACAGCGGGTGTAGACCGACAAAAACATAATGGACCCCAAC10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAGCCAGCAAAAGCAGGCCAAATATATTCA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGGGGGGGGACAGGGATGGTAGATGGAT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAAGTCCTCGTGCTATGGGGCATTCACC10.7299270072992701No Hit
CGCGTGACGTACAGCGAGGGTAAGGGGGCCAATGGAGTAAAAGGATTTTC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTACGACAATTGCATCCGTACCTACTTCGCG10.7299270072992701No Hit
CGCGTGACGTACAGCGCATGTACCTTTCTGACATGAACCTCGAGGAAATG10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTACGGAGCAAATGGAGTAAAAGGATATGCT10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTATTGGACCAGGCGGTCATGGAAAAGAACA10.7299270072992701No Hit
CTCGTGACGTACAGCGCGTGTAGGGGGCGGAACAGGCAGTGTTTATATTT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAATGGAGCAAATGGAGTAAAAGGATATGC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGCACCGAACATTGAAAATGAAGAGAA10.7299270072992701No Hit
CGCGTGACGTACAACGCGTGTAGTGGTCTGGATGCTGAACAAAACTCCCG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGGGTAGCAGAAACAATATATTGAGAAAACAAGC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGGACGAAGAACAAGGGTTGCTTATGAAA10.7299270072992701No Hit
CGCATGACGTACAGCGCGTGTAGGGTGCCAAAAACATAATAGACTCCAAC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGCACCAAACGATCATATGAACAAATG10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTATGGGGTCACCACATCAGTATCATTTTTT10.7299270072992701No Hit
TGCGTGACGTACAGCGCGTGTAGTAGGTACAGATGCAATTGTCGTTCTAA10.7299270072992701No Hit
CACGTGACGTACAGCGCGTGTAAGAAAGGGAAAGATAGTCAAATCAGTCG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAGAGACCCGAATGCACTGTTGAATACAGA10.7299270072992701No Hit
CCCGTGACGTACAGCGCGTGTAGAAAGGGAAAGATAGTCAAATCCGTCGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGGGGGACAAAAACATAATGGACTCCAA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGCGTAGGGGGCCAAATGGATCCAAACAGTGAAA10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAATGACCGATGGACCAAGTGATGGACAGG10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAAAAGCAGGTGTTTAAAATGAATCCAAA10.7299270072992701No Hit
CGTGTGACGTACAGCGCGGGTAAGCAGGCCAAATATCTTCAATCTGGGGA10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAAAGGATTTTCATTCAAATACGGCAATG10.7299270072992701No Hit
CGCGTGCCGTACAGCGCGTGTAGGCCAAGCAATAGGGAAGTGGCTGTGTT10.7299270072992701No Hit
CGCGTGACGTACAGCGCGGGTAGGGGAGGGCATAAAAGGACTGGATGAGC10.7299270072992701No Hit
CGCGTGACGTACAGCGCGTGTAAGGACGGCAATGGTGTTTGGATAGGGAG10.7299270072992701No Hit
CGTGTGACGTACAGCGCGTGTAACAGGAAAAACAAGGGAGTTTTTTACTC10.7299270072992701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACA100.094.999997
GTACAGC100.094.999999
GCGTGAC100.094.999992
GACGTAC100.094.999996
CGTACAG100.094.999998
GTGACGT100.094.999994
CGTGACG100.094.999993
CGCGTGA100.094.999991
TGACGTA100.094.999995