FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC160CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC160CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGACGTACACTCTAGTATCCTACGATATCTTGCTTTATTGAGAAGTT370.6863290669634576No Hit
CGCTGACGTACACTCTAGTATCCTGACCACTCATTTATTCCTACGATATC360.667779632721202No Hit
CGCTGACGTACACTCTAGTATCGGGGGGCAAATGGAGTAAAAGGATTTTC340.6306807642366908No Hit
CGCTGACGTACACTCTAGTATCGGGGGAGCAAATGGAGTAAAAGGATTTT220.4080875533296235No Hit
CGCTGACGTACACTCTAGTATCCTGCGATATCTTGCTTTATTGAGAAGTT170.3153403821183454No Hit
CGCTGACGTACACTCTAGTATCGGGGGGGCAAATGGAGTAAAAGGATTTT170.3153403821183454No Hit
CGCTGACGTACACTCTAGTATCGGGGAGCAAATGGAGTAAAAGGATTTTC150.27824151363383415No Hit
CGCTGACGTACACTCTAGTATCCCGCTATATCCTGACCACTCATTTATTC130.24114264514932293No Hit
CGCTGACGTACACTCTAGTATCATGACCACTCATTTATTCCTACGATATC120.22259321090706735No Hit
CGCTGACGTACACTCTAGTATCCTGAGCACTCATTTATTCCTACGATATC120.22259321090706735No Hit
CGCTGACGTACACTCTAGTATCGCGGGGCAAATGGAGTAAAAGGATTTTC90.1669449081803005No Hit
CGCTGACGTACACTCTAGTATCCAGAGCAGAAATGAAAAGTGGCGAGAGC90.1669449081803005No Hit
CGCTGACGTACACTCTAGTATCCTGCCCACTCATTTATTCCTACGATATC90.1669449081803005No Hit
CGCTGACGTACACTCTAGTATCCTGATCACTCATTTATTCCTACGATATC90.1669449081803005No Hit
CGCTGACGTACACTCTAGTATCCTGAGCAGAAATGAAAAGTGGCGAGAGC80.1483954739380449No Hit
CGCTGACGTACACTCTAGTATCTTGATGTCCCAACAAAGTCTCTATCAGA80.1483954739380449No Hit
CGCTGACGTACACTCTAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGA80.1483954739380449No Hit
CGCTGACGTACACTCTAGTATCGCAGGAGCAAATGGAGTAAAAGGATTTT80.1483954739380449No Hit
CGCTGACGTACACTCTAGTATCAGGGAGCAAATGGAGTAAAAGGATTTTC70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCCTTCGATATCTTGCTTTATTGAGAAGTT70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCCAGCCAGCAATGTTACATTTACCCAAAT70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCGGAGGAGCAAATGGAGTAAAAGGATTTT70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCAAGGAGCAAATGGAGTAAAAGGATTTTC70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCCGGACCACTCATTTATTCCTACGATATC70.12984603969578928No Hit
CGCTGACGTACACTCTAGTATCGCGGAGCAAATGGAGTAAAAGGATTTTC60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCGAAGGAGCAAATGGAGTAAAAGGATTTT60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCATGCGAACAATTCAACAGACACTGTAGA60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCATTAAATAAGCTGAAACGAGAAAGCTCT60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCGACGGAGCAAATGGAGTAAAAGGATTTT60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCAACATCCACAGCACTCTGCTGTTCCTGT60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCGCGGGAGCAAATGGAGTAAAAGGATTTT60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCGTTTAATGGAGCAAATGGAGTAAAAGGA60.11129660545353368No Hit
CGCTGACGTACACTCTAGTATCAAGGGAGCAAATGGAGTAAAAGGATTTT60.11129660545353368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT156.2606455E-495.01
CGCTGAC4700.095.01
TATACTC156.2606455E-495.09
GATGTAC156.2606455E-495.05
ACGTATA156.2606455E-495.06
GACGGAC301.0128133E-895.05
ACGTACA4350.095.06
GGACACT301.0128133E-895.08
CTGACGT4350.095.03
CTGACGG301.0128133E-895.03
CTGATGT156.2606455E-495.03
CGGACAC301.0128133E-895.07
GACACTC301.0128133E-895.09
GTATACT156.2606455E-495.08
GACGTAT156.2606455E-495.05
GACGTAC4300.095.05
CGTACAC4300.095.07
CGTATAC156.2606455E-495.07
GCTGATG156.2606455E-495.02
GCTGACG4650.095.02
Produced by FastQC (version 0.11.5)