FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC154CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC154CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT90.29771749917300694No Hit
CGTCACGTCGTGTGCGTGCTACAATCCCAATTATACTTGCGGCAACAACA80.2646377770426729No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTATTTT70.23155805491233872No Hit
CGTCACGTCGTGTGCGTGCTACGAAAGGGGAAGGTTACTAAATCAATAGA70.23155805491233872No Hit
CGTCACGTCGTGTGCGTGCTACAGTAGAAACAAGGGTATTTTTCATTAAT60.19847833278200464No Hit
CGTCACGTCGTGTGCGTGCTACGGGGGGAGAATGGAAGAAAAACAAGGAT50.16539861065167052No Hit
CGTCACGTCGTGTGCGTGCTACAACTATTGCTTCGACTATTGATTGTTCG50.16539861065167052No Hit
CGTCACGTCGTGTGCGTGCTACCTCAGCCGTCACGTCGTGTGCGTGCTAC50.16539861065167052No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGGGTTTT40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAAGGGAACAACTAGGTCAGAAGTTTGAA40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGGGGAAGGTTACTAAATCAATAGA40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACATCCAAATGTGCACCGAGCTTAAGCTCA40.13231888852133644No Hit
CGTCACGTCGTGTGCGTGCTACAAAAGCAGGTAGATATTGAAAGATGAGT40.13231888852133644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG155.98774E-495.01
TGTCACG155.98774E-495.01
TCGTGGG253.5882476E-795.08
TACGTCG155.98774E-495.04
GACGTCG155.98774E-495.04
GTGACGT155.98774E-495.02
CGTGACG155.98774E-495.01
GTTACGT155.98774E-495.02
TTACGTC155.98774E-495.03
TGACGTC155.98774E-495.03
GTCGTGG201.4746613E-594.999997
ACGTCGT2800.093.303575
CGTCGTG2800.093.303576
GTCGTGT2600.093.173087
TCGTGTG2600.093.173088
CACGTCG2400.093.0208364
CGTGTGC2750.091.5454569
TCACGTC2400.091.041673
GTCACGT2150.090.58142
CGTCACG2100.090.476191
Produced by FastQC (version 0.11.5)