FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC152CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC152CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13194
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAGAGATATAGACGTAGAATGAGAAAATGTGAATGTGTGTCTTCTC1180.894345914809762No Hit
CGCGTAGAGATATAGACGTAGAAAAAGCAATGAAAGAGTATGGAGAGGAC710.538123389419433No Hit
CGCGTAGAGATATAGACGTAGACAGAGCAGTCAAACTTTATCGAAAGCTT570.43201455206912237No Hit
CGCGTAGAGATATAGACGTAGAAACAGCTACTTGTGTTGGAAAGCAAATA490.37138093072608763No Hit
CGCGTAGAGATATAGACGTAGATATTGAAAGATGAGTCTTCTAACCGAGG350.26527209337577684No Hit
CGCGTAGAGATATAGACGTAGAAAAAGCAGGTAGATATTGAAAGATGAGT340.2576928907078975No Hit
CGCGTAGAGATATAGACGTAGAAGGCGCTCTCTTTAAACACGGTTTAAAA340.2576928907078975No Hit
CGCGTAGAGATATAGACGTAGAAGGCCAATTATATTCAATATGGAAAGAA250.18948006669698347No Hit
CGCGTAGAGATATAGACGTAGAACCCAAATCAAAAGATAATAACCATTGG240.18190086402910413No Hit
CGCGTAGAGATATAGACGTAGAAACAAAGTAGTTTTTTACTCTAGCTCTA230.1743216613612248No Hit
CGCGTAGAGATATAGACGTAGATATTGCAAGATGAGTCTTCTAACCGAGG220.16674245869334545No Hit
CGCGTAGAGATATAGACGTAGAAAAGCAGGAAGATATTGAAAGATGAGTC190.14400485068970745No Hit
CGCGTAGAGATATAGACGTAGAAGGCGCTCTTTTTAAACACGGTTTAAAA180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGACCGAGAGCGATGCCCCCTTCCTTGATCG180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGATATTGGAAGATGAGTCTTCTAACCGAGG180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGAAGGGGGGCAAAGACATAATGGATTCCCA180.1364256480218281No Hit
CGCGTAGAGATATAGACGTAGATGAGTCTTCTAACCGAGGTCGAAACGTA170.12884644535394876No Hit
CGCGTAGAGATATAGACGTAGAGAGGGGTTTGAGATGATTTGGGATCCTA170.12884644535394876No Hit
CGCGTAGAGATATAGACGTAGAAGCAAAAGCAGGTAGATATTGAAAGATG160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGAAGCCGAGATCGCACAGAGACTTGAAGAT160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGACTGCTCTGTCCAAAATGCCCTTAATGGG160.12126724268606943No Hit
CGCGTAGAGATATAGACGTAGATGTTGAAAGATGAGTCTTCTAACCGAGG150.11368804001819009No Hit
CGCGTAGAGATATAGACGTAGAGGGGGGCGAAGACATAATGGATTCCCAC150.11368804001819009No Hit
CGCGTAGAGATATAGACGTAGAAAAACCCATCACTTAGGATGAAATGGAT150.11368804001819009No Hit
CGCGTAGAGATATAGACGTAGAAGGCACTGATTCAAAATGGAAGATTTTG150.11368804001819009No Hit
CGCGTAGAGATATAGACGTAGATTTTTGGATAGTGAATAGTACTTGCCAC140.10610883735031075No Hit
CGCGTAGAGATATAGACGTAGATTTTGAAAGATGAGTCTTCTAACCGAGG140.10610883735031075No Hit
CGCGTAGAGATATAGACGTAGAAGGGGGCAAAGACATAATGGATTCCCAC140.10610883735031075No Hit
CGCGTAGAGATATAGACGTAGAAGGCCCTCTTTTTAAACCGTGTTTAAAG140.10610883735031075No Hit
CGCGTAGAGATATAGACGTAGAAGGACCAAAAGTAACAGACTTAGAAAGG140.10610883735031075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATG201.6802354E-595.000016
CGTAGGG254.370031E-795.03
GGATATA254.370031E-795.08
GCGTGGA156.4721337E-495.02
CGTGGAG156.4721337E-495.03
TAGAGAT12000.095.05
GTAGGGA254.370031E-795.04
GCGTAGG254.370031E-795.02
TAGGGAT254.370031E-795.05
CGCGTGG156.4721337E-495.01
CGCGTAG12200.095.01
GATATAG12350.094.615399
GAGATAT12150.094.6090557
AGAGATA12000.094.604176
GCGTAGA12200.094.2213062
AGATATA12100.094.2148748
GTAGAGA13250.086.754724
AGGGATA301.2934361E-679.166676
GGGATAT301.2934361E-679.166677
GTGTAGA255.0837793E-576.02
Produced by FastQC (version 0.11.5)