FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC149CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC149CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310653
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATAGACGACTAGTACGTGAATGAGAAAATGTGAATGTGTGTCTTCTC32481.045539556997679No Hit
CGTATAGACGACTAGTACGTGAAGGACCAAAAGTAACAGACTTAGAAAGG16840.5420839328768755No Hit
CGTATAGACGACTAGTACGTGAGGGGCGGGAACAACTAGGTCAGAAGTTT14160.4558140433216483No Hit
CGTATAGACGACTAGTACGTGAATGAGCAAATGTGAATGTGTGTCTTCTC9100.2929313414002118No Hit
CGTATAGACGACTAGTACGTGAACCCAAATCAAAAGATAATAACCATTGG8910.28681519251383375No Hit
CGTATAGACGACTAGTACGTGAAGGGCGGGAACAACTAGGTCAGAAGTTT8290.2668572329898633No Hit
CGTATAGACGACTAGTACGTGAGGGGCCGCAAGTATAATTGGGATTGTGC8170.2629944021142561No Hit
CGTATAGACGACTAGTACGTGAAGGGGGGGAACAACTAGGTCAGAAGTTT7760.24979639662259823No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGGTGTTTTTTATCA7530.24239263744435108No Hit
CGTATAGACGACTAGTACGTGACGCCACAAAAAGAAATGCTGCTCCCACT7430.23917361171467844No Hit
CGTATAGACGACTAGTACGTGACTTAGAAAGGGGTTTGAGATGATTTGGG6710.2159966264610353No Hit
CGTATAGACGACTAGTACGTGACCCAGAAGCAAGGTCTTATACAGTCCAA6620.21309950330432992No Hit
CGTATAGACGACTAGTACGTGAAACAGGATGTTGGACGAAACTTCCGCTG5700.18348446659134146No Hit
CGTATAGACGACTAGTACGTGACTTCCAGTTTGGATTGAGTGACTAGCCC5320.17125216881858535No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGTAGTTTTTTACTC5240.16867694823484725No Hit
CGTATAGACGACTAGTACGTGATTGTGCACTTGATATTGTGGATTATTGA5180.16674553279704363No Hit
CGTATAGACGACTAGTACGTGAATGTGTGTCTTCTCAGATTTAATTTTGT5170.16642363022407639No Hit
CGTATAGACGACTAGTACGTGAGGGGGGGGAACAACTAGGTCAGAAGTTT4900.1577322607539602No Hit
CGTATAGACGACTAGTACGTGAAAACCCTTTTACTCCGTTTGCTCCATCA4880.15708845560802567No Hit
CGTATAGACGACTAGTACGTGAACCCGAATCAAAAGATAATAACCATTGG4710.15161611186758217No Hit
CGTATAGACGACTAGTACGTGAAAGGGGTTTGAGATGATTTGGGATCCTA4700.1512942092946149No Hit
CGTATAGACGACTAGTACGTGAACACAAATCCTAAAATCCCCTTAGTCAG4530.1458218655541714No Hit
CGTATAGACGACTAGTACGTGACCGAGAGTGCTGCCTCTTCCCTTTAGAG4530.1458218655541714No Hit
CGTATAGACGACTAGTACGTGACAGCCCATATTGAAATAATATTTCCTAT4220.13584288579218615No Hit
CGTATAGACGACTAGTACGTGAACGCCACAAAAAGAAATGCTGCTCCCAC4200.1351990806462516No Hit
CGTATAGACGACTAGTACGTGACTGCCCATATTGAAATAATATTTCCTAT4140.13326766520844802No Hit
CGTATAGACGACTAGTACGTGATTTGGGATCCTAATGGATGGACAAATAC4010.12908293175987356No Hit
CGTATAGACGACTAGTACGTGAACAAGCAGCTGAGGCCATGGAGGTTGCT3590.1155630236952484No Hit
CGTATAGACGACTAGTACGTGACGGGCGGGAACAACTAGGTCAGAAGTTT3570.11491921854931388No Hit
CGTATAGACGACTAGTACGTGACAGAGCAGTCAAACTTTATCGAAAGCTT3570.11491921854931388No Hit
CGTATAGACGACTAGTACGTGAGGGGCCCAAATGCACTTTTAAAGCACAG3530.1136316082574448No Hit
CGTATAGACGACTAGTACGTGACCCAAATGCACTTTTAAAGCACAGATTT3400.10944687480887036No Hit
CGTATAGACGACTAGTACGTGAAAGAAATTTCAGTGTCCTGGGAGTTGGT3300.1062278490791977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG1150.095.0739441
CCTATAG409.094947E-1295.0739441
CGTATTG156.5929175E-495.073941
TAGACGG650.094.997435
CGGCTAG650.094.997439
CTAGACG254.613412E-794.997424
CTATAGA409.094947E-1294.997422
GTCTAGA254.613412E-794.997422
GTATAGG1200.094.997422
CGACTTG353.2923708E-1094.997429
CGTATAG305150.093.7653661
ATAGACG301800.093.6124344
TATAGAC302700.093.600873
TAGACGA301150.093.593685
GACGACT300500.093.574847
GTATAGA304050.093.56022
ACGACTA299600.093.538868
CGACTAG301750.093.439069
ACGACTC1100.090.679368
TGTATAG800.089.131821
Produced by FastQC (version 0.11.5)