FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC139CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC139CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66528
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGTAGATACGACAGAGAGATGAGTCTTCTAACCGAGGTCGAAACGTA3930.5907287157287157No Hit
CGTAGTAGATACGACAGAGAGAGGGGTTTGAGATGATTTGGGATCCTAAT2880.4329004329004329No Hit
CGTAGTAGATACGACAGAGAGAATGAGAAAATGTGAATGTGTGTCTTCTC2280.3427128427128427No Hit
CGTAGTAGATACGACAGAGAGACGCCACAAAAAGAAATGCTGCTCCCACT2050.3081409331409331No Hit
CGTAGTAGATACGACAGAGAGAAACAGCTACTTGTGTTGGAAAGCAAATA2030.30513468013468015No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCCTGACGGGATGATAGAGAGAAC1810.27206589706589707No Hit
CGTAGTAGATACGACAGAGAGAAGTAGAAACAAGGTAGTTTTTTACTCTA1620.2435064935064935No Hit
CGTAGTAGATACGACAGAGAGAAGGGGTTTGAGATGATTTGGGATCCTAA1570.23599086099086097No Hit
CGTAGTAGATACGACAGAGAGAAAAAGCAATGAAAGAGTATGGAGAGGAC1550.232984607984608No Hit
CGTAGTAGATACGACAGAGAGAAGCAAAAGCAGGTAGATATTGAAAGATG1510.22697210197210196No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCTGTGACCACCGAATCAGCATTT1370.20592833092833093No Hit
CGTAGTAGATACGACAGAGAGAACCCAAATCAAAAGATAATAACCATTGG1350.2029220779220779No Hit
CGTAGTAGATACGACAGAGAGACAGAGCAGTCAAACTTTATCGAAAGCTT1190.17887205387205388No Hit
CGTAGTAGATACGACAGAGAGAATAACCATTGGATCAATCAGTATAGCAA1160.17436267436267436No Hit
CGTAGTAGATACGACAGAGAGAAAAAGCAGGTAGATATTGAAAGATGAGT1080.16233766233766234No Hit
CGTAGTAGATACGACAGAGAGAAAGGGGTTTGAGATGATTTGGGATCCTA1010.15181577681577682No Hit
CGTAGTAGATACGACAGAGAGAGAGGGGTTTGAGATGATTTGGGATCCTA990.1488095238095238No Hit
CGTAGTAGATACGACAGAGAGAGGGGGTGCAGATGCAACGATTCAAGTGA970.1458032708032708No Hit
CGTAGTAGATACGACAGAGAGAGGGAGATAACATTCCATGGGGCCAAAGA950.1427970177970178No Hit
CGTAGTAGATACGACAGAGAGATGGGGGTGCAGATGCAACGATTCAAGTG910.1367845117845118No Hit
CGTAGTAGATACGACAGAGAGAGGGGGATAACATTCCATGGGGCCAAAGA900.13528138528138528No Hit
CGTAGTAGATACGACAGAGAGAAGGACCAAAAGTAACAGACTTAGAAAGG870.13077200577200576No Hit
CGTAGTAGATACGACAGAGAGAGGGGGGCTGTGACCACCGAATCAGCATT870.13077200577200576No Hit
CGTAGTAGATACGACAGAGAGACTTGAAGATGTATTTGCTGGAAAGAATA870.13077200577200576No Hit
CGTAGTAGATACGACAGAGAGAAGGCGAATCAATAATAGTAGAGCCTGAG850.12776575276575278No Hit
CGTAGTAGATACGACAGAGAGAAAGCAAATAGTAGAGAGGATTCTGAAAG830.12475949975949976No Hit
CGTAGTAGATACGACAGAGAGAAGCAAAAGCAGGCAGATATTGAAAGATG790.11874699374699374No Hit
CGTAGTAGATACGACAGAGAGAACGCCACAAAAAGAAATGCTGCTCCCAC780.11724386724386725No Hit
CGTAGTAGATACGACAGAGAGACCCAGATGCACTTTTAAAGCACAGATTT760.11423761423761424No Hit
CGTAGTAGATACGACAGAGAGAAGCCGAGATCGCACAGAGACTTGAAGAT750.11273448773448773No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCAATTGTTGGCGAAATTTCACCA750.11273448773448773No Hit
CGTAGTAGATACGACAGAGAGAAGAAGATGTATTTGCTGGAAAGAATACC740.11123136123136124No Hit
CGTAGTAGATACGACAGAGAGAGGGGGGCAAAGACATAATGGATTCCCAC730.10972823472823472No Hit
CGTAGTAGATACGACAGAGAGAGGGGGCGAAACGTACGTTCTCTCTATCA720.10822510822510822No Hit
CGTAGTAGATACGACAGAGAGAGAGGGCGAAACGTACGTTCTCTCTATCA720.10822510822510822No Hit
CGTAGTAGATACGACAGAGAGAGGTCGAAACGTACGTTCTCTCTATCATC700.10521885521885521No Hit
CGTAGTAGATACGACAGAGAGAGCAGGTAGATATTGAAAGATGAGTCTTC690.10371572871572872No Hit
CGTAGTAGATACGACAGAGAGAAGGGGGCAAAGACATAATGGATTCCCAC680.10221260221260221No Hit
CGTAGTAGATACGACAGAGAGAAGTAGAAACAAGGCAGTTTTTTACTCTA680.10221260221260221No Hit
CGTAGTAGATACGACAGAGAGAGGGGGATACTTGAAGATGAGCAAATGTA670.10070947570947572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA301.1954398E-895.212511
CGCAGTA156.531963E-495.212511
GGTAGTA201.7125862E-595.212511
GATACGG254.5732668E-794.997758
GGTACGA156.590924E-494.997758
GCAGTAG156.590924E-494.997752
GATGCGA254.5732668E-794.997758
ATGCGAC254.5732668E-794.997759
ATACGGC254.5732668E-794.997759
TAGTAGG156.590924E-494.997753
AGATCCG201.731925E-594.997747
ATAGGAC201.731925E-594.997749
GATCCGA201.731925E-594.997748
TAGATCC201.731925E-594.997746
CGTAGTA64500.094.695851
GTAGTAG64850.094.485032
TAGATAC64650.094.483456
ATACGAC64050.094.478639
AGTAGAT65500.093.98254
GATACGA64750.093.970758
Produced by FastQC (version 0.11.5)