FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC136CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC136CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815722
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGAGTACGATATATACTGAAACGAAAACGTTCTAATCTCTTGTTCC122291.4991627049411442No Hit
CGTATGAGTACGATATATACTGATTCAAAATGGAAGATTTTGTACGACAA54430.6672616406079522No Hit
CGTATGAGTACGATATATACTGAAGACCCAGATGAAGGCACAGCTGGAGT19250.2359872603656638No Hit
CGTATGAGTACGATATATACTGATCAGCAAAAGCAGGTCTCCCCATGCCT17950.22005045836694365No Hit
CGTATGAGTACGATATATACTGGGGGACCTCCATTCACTACAACACAGAA17940.2199278675823381No Hit
CGTATGAGTACGATATATACTGTGGGGAGGGGCCAGGAGGCTGAGGGAGT16630.2038684747990124No Hit
CGTATGAGTACGATATATACTGGGGGCGGGAACAACTAGGTCAGAAGTTT14070.17248523393999426No Hit
CGTATGAGTACGATATATACTGGGGGGAACAACTAGGTCAGAAGTTTGAA13400.1642716513714231No Hit
CGTATGAGTACGATATATACTGTGTGGGGAGGGGCCAGGAGGCTGAGGGA11870.14551526132677556No Hit
CGTATGAGTACGATATATACTGAGAACAGAATGGTTCTGGCCAGCACTAC11550.14159235621939828No Hit
CGTATGAGTACGATATATACTGCAAACAGAATATGGATAAAGTGGTAGGA10990.13472727228148804No Hit
CGTATGAGTACGATATATACTGGAGGCGGGAACAACTAGGTCAGAAGTTT8780.1076347088836638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA804350.095.028041
GTACGGT1650.094.996148
GTACGAT797250.094.9186868
AGTACGA797800.094.900887
GAGTACG799550.094.8654566
TACGATA797600.094.823439
TGAGTAC800750.094.7351465
ATGAGTA802200.094.717854
TATGAGT804400.094.5650943
GTATGAG808900.094.532252
GGACGAT3800.089.996348
AGTACGG1750.089.5677957
GTGCGAT2200.086.360128
TACGATT500.085.496539
GAGTTCG500.085.496536
TACGGTA1850.084.726299
CGTATTA452.5465852E-1184.669371
TAGTACG409.531504E-1083.121626
CGTATGT353.524292E-881.645461
GACGATA4250.081.584929
Produced by FastQC (version 0.11.5)